Triciferol
Based on 1 Customer Validation
Triciferol functions as a multiple ligand with combined VDR agonist and HDAC antagonist activities. Triciferol binds directly to the VDR (IC50=87 nM), and functions as an agonist with 1,25D-like potency on several 1,25D target genes. Triciferol induces marked tubulin hyperacetylation, and augments histone acetylation. Antiproliferative and cytotoxic activities.
For research use only. We do not sell to patients.
- Purity : 98.61%
- CAS No.: 957214-00-5
- Formula: C26H39NO4
- Molecular Weight:429.59
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-10002
CalcitriolVDR Agonist -
HY-15398
Vitamin D3Vitamin -
HY-15398R
Vitamin D3 (Standard)Vitamin -
HY-10002R
Calcitriol (Standard)VDR Agonist -
HY-32351
CalcifediolVDR Ligand -
HY-32350
ErcalcitriolVDR Agonist -
HY-76801
24, 25-Dihydroxy VD2Metabolite -
HY-32349
ErcalcidiolVitamin D2 Metabolite -
HY-76915
24, 25-Dihydroxy VD3Vitamin D3 -
HY-32351R
Calcifediol (Standard)VDR Ligand -
HY-32344
InecalcitolVDR Agonist -
HY-32350R
Ercalcitriol (Standard)Endogenous Metabolite
Biological Activity
Description
In Vitro
Triciferol (0-10000 nM; 0-72 hours) is significantly more efficacious in suppressing the proliferation of estrogen receptor-negative human MDA-MB231 breast cancer cells[1].
Treatment of MCF-7 cells with Triciferol (100-1000 nM) induces ≈2.5-fold higher rates of cell death than equimolar amounts of 1,25D[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDA-MB231 breast cancer cells
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Concentration:0-10000 nM
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Incubation Time:0-72 hours
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Result:Growth inhibition was statistically significantly different from that of 1,25D at concentrations of 1 nM or above.
Chemical Information
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CAS No. 957214-00-5
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Appearance Solid
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Molecular Weight 429.59
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Formula C26H39NO4
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Color White to off-white
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SMILES
C[C@@H]([C@H]1CC[C@@]2([H])/C(CCC[C@]12C)=C/C=C3C[C@@H](O)C[C@H](O)C/3)/C=C(C)/C=C/C(NO)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (232.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3278 mL | 11.6390 mL | 23.2780 mL | 58.1950 mL |
| 5 mM | 0.4656 mL | 2.3278 mL | 4.6556 mL | 11.6390 mL | |
| 10 mM | 0.2328 mL | 1.1639 mL | 2.3278 mL | 5.8195 mL | |
| 15 mM | 0.1552 mL | 0.7759 mL | 1.5519 mL | 3.8797 mL | |
| 20 mM | 0.1164 mL | 0.5820 mL | 1.1639 mL | 2.9098 mL | |
| 25 mM | 0.0931 mL | 0.4656 mL | 0.9311 mL | 2.3278 mL | |
| 30 mM | 0.0776 mL | 0.3880 mL | 0.7759 mL | 1.9398 mL | |
| 40 mM | 0.0582 mL | 0.2910 mL | 0.5820 mL | 1.4549 mL | |
| 50 mM | 0.0466 mL | 0.2328 mL | 0.4656 mL | 1.1639 mL | |
| 60 mM | 0.0388 mL | 0.1940 mL | 0.3880 mL | 0.9699 mL | |
| 80 mM | 0.0291 mL | 0.1455 mL | 0.2910 mL | 0.7274 mL | |
| 100 mM | 0.0233 mL | 0.1164 mL | 0.2328 mL | 0.5820 mL |
Triciferol Related Classifications
HY-131961 Related Classifications
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