UNC2400
Based on 1 Customer Validation
UNC2400 is a close analog of UNC1999 with >1,000-fold lower potency than UNC1999 as a negative control for cell-based studies.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 1433200-49-7
- Formula: C35H47N7O2
- Molecular Weight:597.79
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Histone Methyltransferase Isoforms
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF-10A | EC50 |
27500 nM
Compound: Negative control: UNC2400
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Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability by alamar blue assay
Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability by alamar blue assay
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[PMID: 23614352] |
Chemical Information
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CAS No. 1433200-49-7
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Appearance Solid
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Molecular Weight 597.79
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Formula C35H47N7O2
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Color White to off-white
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SMILES
O=C(C1=CC(C2=CC=C(N3CCN(C(C)C)CC3)N=C2)=CC4=C1C=NN4C(C)C)N(CC5=C(CCC)C=C(C)N(C)C5=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 65 mg/mL (108.73 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Kyle D Konze, et al. An orally bioavailable chemical probe of the Lysine Methyltransferases EZH2 and EZH1. ACS Chem Biol. 2013;8(6):1324-34. [Content Brief]
[2]. Bowen Xu, et al. Selective inhibition of EZH2 and EZH1 enzymatic activity by a small molecule suppresses MLL-rearranged leukemia. Blood. 2015 Jan 8;125(2):346-57. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6728 mL | 8.3641 mL | 16.7283 mL | 41.8207 mL |
| 5 mM | 0.3346 mL | 1.6728 mL | 3.3457 mL | 8.3641 mL | |
| 10 mM | 0.1673 mL | 0.8364 mL | 1.6728 mL | 4.1821 mL | |
| 15 mM | 0.1115 mL | 0.5576 mL | 1.1152 mL | 2.7880 mL | |
| 20 mM | 0.0836 mL | 0.4182 mL | 0.8364 mL | 2.0910 mL | |
| 25 mM | 0.0669 mL | 0.3346 mL | 0.6691 mL | 1.6728 mL | |
| 30 mM | 0.0558 mL | 0.2788 mL | 0.5576 mL | 1.3940 mL | |
| 40 mM | 0.0418 mL | 0.2091 mL | 0.4182 mL | 1.0455 mL | |
| 50 mM | 0.0335 mL | 0.1673 mL | 0.3346 mL | 0.8364 mL | |
| 60 mM | 0.0279 mL | 0.1394 mL | 0.2788 mL | 0.6970 mL | |
| 80 mM | 0.0209 mL | 0.1046 mL | 0.2091 mL | 0.5228 mL | |
| 100 mM | 0.0167 mL | 0.0836 mL | 0.1673 mL | 0.4182 mL |
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