Cat. No.: HY-119769

XX-650-23

Customer Review

Based on 1 Customer Validation

XX-650-23 is a potent CREB inhibitor. XX-650-23inhibits CREB function through disruption of CBP-CREB interaction. XX-650-23 can be used for AML research.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 117739-40-9
Formula: C₁₈H₁₂N₂O₂
Molecular Weight:288.30

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

XX-650-23 Chemical Structure

CAS No.: 117739-40-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

XX-650-23 is a potent CREB inhibitor. XX-650-23inhibits CREB function through disruption of CBP-CREB interaction. XX-650-23 can be used for AML research^[1].

All Histone Acetyltransferase Isoforms :

All Epigenetic Reader Domain Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Bone marrow cell	IC₅₀	>10 μM Compound: 3; XX-650-23	Cytotoxicity against human bone marrow cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human bone marrow cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
HEK293	IC₅₀	4.03 μM Compound: 3; XX-650-23	Inhibition of KIX-RLucN fused CBP (unknown origin) binding to KID-RLucC fused CREB (unknown origin) transfected in human HEK293 cells preincubated with compound 30 mins before before forskolin addition and measured after 90 mins in presence of coelenteraz Inhibition of KIX-RLucN fused CBP (unknown origin) binding to KID-RLucC fused CREB (unknown origin) transfected in human HEK293 cells preincubated with compound 30 mins before before forskolin addition and measured after 90 mins in presence of coelenteraz	[PMID: 31253529]
HL-60	IC₅₀	1.58 μM Compound: 3; XX-650-23	Cytotoxicity against human HL60 cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human HL60 cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
KG-1	IC₅₀	1.23 μM Compound: 3; XX-650-23	Cytotoxicity against human KG1 cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human KG1 cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]

In Vitro

XX-650-23 (870 nM-2.3 μM; 48 hours) inhibits AML cell growth, the IC₅₀ values are 870 nM, 910 nM, 2.0 μM and 2.3 μM for HL-60, KG-1, MOLM-13, and MV-4-11,respectively^[1].
XX-650-23( 5 μM; 6 or 24 hours) causes a specific decrease in H3K27 acetylation and is not a general inhibitor of acetyltransferase activity^[1].
XX-650-23 (2 μM; 72 hours) elicits apoptosis through the intrinsic apoptosis pathway, with activation of caspase-3 and detectable caspase-9 cleavage. It also leads to the downregulation of Mcl-1 and bcl-2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

KG-1 cells
Concentration:

5 μM
Incubation Time:

6 or 24 hours
Result:

Decreased H3K27 acetylation expression.

Apoptosis Analysis^[1]

Cell Line:

HL-60 cells
Concentration:

2 μM
Incubation Time:

72 hours
Result:

Induced apoptosis in AML cells.

Chemical Information

CAS No. 117739-40-9
Appearance Solid
Molecular Weight 288.30
Formula C₁₈H₁₂N₂O₂
Color Off-white to yellow
SMILES

O=C(C1=C(C=C2C=CC=CC2=C1)O)NC3=CC=C(C=C3)C#N
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (346.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4686 mL

17.3430 mL

34.6861 mL

5 mM

0.6937 mL

3.4686 mL

6.9372 mL

10 mM

0.3469 mL

1.7343 mL

3.4686 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (8.67 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. B Mitton, et al. Small molecule inhibition of cAMP response element binding protein in human acute myeloid leukemia cells. Leukemia. 2016 Dec;30(12):2302-2311. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4686 mL	17.3430 mL	34.6861 mL	86.7152 mL
	5 mM	0.6937 mL	3.4686 mL	6.9372 mL	17.3430 mL
	10 mM	0.3469 mL	1.7343 mL	3.4686 mL	8.6715 mL
	15 mM	0.2312 mL	1.1562 mL	2.3124 mL	5.7810 mL
	20 mM	0.1734 mL	0.8672 mL	1.7343 mL	4.3358 mL
	25 mM	0.1387 mL	0.6937 mL	1.3874 mL	3.4686 mL
	30 mM	0.1156 mL	0.5781 mL	1.1562 mL	2.8905 mL
	40 mM	0.0867 mL	0.4336 mL	0.8672 mL	2.1679 mL
	50 mM	0.0694 mL	0.3469 mL	0.6937 mL	1.7343 mL
	60 mM	0.0578 mL	0.2891 mL	0.5781 mL	1.4453 mL
	80 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0839 mL
	100 mM	0.0347 mL	0.1734 mL	0.3469 mL	0.8672 mL

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XX-650-23

Bioactive Compound Library Max

Bioactive Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Reprogramming Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Pain-Related Compound Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

MCE Bioactive Compound Library

High-Throughput Bioactive Compound Library

All Histone Acetyltransferase Isoforms

All Epigenetic Reader Domain Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

XX-650-23

Bioactive Compound Library Max

Bioactive Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Reprogramming Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Pain-Related Compound Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

MCE Bioactive Compound Library

High-Throughput Bioactive Compound Library

All Histone Acetyltransferase Isoforms

All Epigenetic Reader Domain Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

XX-650-23 Related Classifications

HY-119769 Related Classifications