1. Apoptosis
  2. Caspase

2-HBA 

Cat. No.: HY-103667 Purity: 98.83%
Handling Instructions

2-HBA is a potent inducer of NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1) which can also activate caspase-3 and caspase-10.

For research use only. We do not sell to patients.

2-HBA Chemical Structure

2-HBA Chemical Structure

CAS No. : 131359-24-5

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
5 mg USD 72 In-stock
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10 mg USD 108 In-stock
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25 mg USD 234 In-stock
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50 mg USD 432 In-stock
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100 mg USD 732 In-stock
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

2-HBA is a potent inducer of NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1) which can also activate caspase-3 and caspase-10.

IC50 & Target[1]

Caspase-3

 

Caspase-10

 

In Vitro

When L1210 cells are exposed to 0.6 μM 2-HBA (bis(2-hydroxy-benzylidene)acetone), the specific activities of NQO1 and glutathione reductase increase by 6- and 1.5-fold, respectively. The total cellular glutathione content is also coordinately induced by 2.4-fold. In a more detailed study it is found that NQO1 is induced by 2-HBA in a concentration-dependent manner. Treatments with 2-HBA cause cell cycle arrest and apoptosis in both L1210 wild type cells and their Y8 drug-resistant counterparts in a concentration-dependent manner. 2-HBA can also activate caspase-3 and caspase-10[1].

Solvent & Solubility
In Vitro: 

DMSO : 107 mg/mL (401.82 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7553 mL 18.7765 mL 37.5530 mL
5 mM 0.7511 mL 3.7553 mL 7.5106 mL
10 mM 0.3755 mL 1.8777 mL 3.7553 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

Cells (20,000 per well) are grown for 24 h in 96-well plates, then exposed to 2-HBA (bis(2-hydroxybenzylidene)acetone) for either 24 h (for glutathione determination) or 48 h (for determination of enzyme activities). At the end of the exposure period, cells are collected by centrifugation (1500×g for 15 min at 4°C), washed with DPBS, and finally lysed in 0.08% digitonin. An aliquot (25 μL) is used for protein analysis. Activity of NQO1 is determined by the Prochaska test[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

After exposure to 2-HBA (bis(2-hydroxybenzylidene)acetone) for 24 h, duplicate aliquots of cells (1×106) are collected by centrifugation and washed with cold DPBS. Apoptosis is determined using the Annexin-V-FLUOS assay with simultaneous determination of the necrotic fraction by the uptake of propidium iodide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

266.29

Formula

C₁₇H₁₄O₃

CAS No.

131359-24-5

SMILES

O=C(/C=C/C1=CC=CC=C1O)/C=C/C2=CC=CC=C2O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
2-HBA
Cat. No.:
HY-103667
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2-HBA

Cat. No.: HY-103667