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MT-DADMe-ImmA (Synonyms: MTDIA; Methylthio-DADMe-Immucillin A)

Cat. No.: HY-101496 Purity: 99.39%
Handling Instructions

MT-DADMe-ImmA is an inhibitor of human 5'-methylthioadenosine phosphorylase (MTAP) with a Ki of 90 pM.

For research use only. We do not sell to patients.

MT-DADMe-ImmA Chemical Structure

MT-DADMe-ImmA Chemical Structure

CAS No. : 653592-04-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 185 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 185 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 168 In-stock
Estimated Time of Arrival: December 31
10 mg USD 288 In-stock
Estimated Time of Arrival: December 31
25 mg USD 540 In-stock
Estimated Time of Arrival: December 31
50 mg USD 960 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1680 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MT-DADMe-ImmA

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  • Customer Review

Description

MT-DADMe-ImmA is an inhibitor of human 5'-methylthioadenosine phosphorylase (MTAP) with a Ki of 90 pM.

IC50 & Target

Ki: 90 pM (MTAP)[1]

In Vitro

Treatment of cultured cells with MT-DADMe-ImmA and MTA inhibit MTAP, increase cellular MTA concentrations, decrease polyamines, and induce apoptosis in FaDu and Cal27, two head and neck squamous cell carcinoma cell lines. The same treatment does not induce apoptosis in normal human fibroblast cell lines (CRL2522 and GM02037) or in MCF7, a breast cancer cell line with an MTAP gene deletion. MT-DADMe-ImmA alone does not induce apoptosis in any cell line, implicating MTA as the active agent[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The t1/2 for onset of inhibition is 50 min with complete inhibition by 250 min. MTAP activity slowly returns, giving a biological half-life for the action of oral MT-DADMe-ImmA of 6.3 days. The time-dependent growth of FaDu tumors in immunodeficient mice is suppressed by oral or intraperitoneal treatment with MT-DADMe-ImmA[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

293.39

Formula

C13H19N5OS

CAS No.
SMILES

O[[email protected]]1CN(CC2=CNC3=C(N)N=CN=C32)C[[email protected]@H]1CSC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (340.84 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4084 mL 17.0422 mL 34.0843 mL
5 mM 0.6817 mL 3.4084 mL 6.8169 mL
10 mM 0.3408 mL 1.7042 mL 3.4084 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.52 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.52 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.52 mM); Clear solution

*All of the co-solvents are available by MCE.
References
Cell Assay
[2]

Cell viability is evaluated using the Alamar Blue assay. Cells are seeded onto 96-well plates at a density of 104 cells/well and incubated with increasing concentrations of MT-DADMe-ImmA (100 pM to 100 μM) for 4 days at fixed MTA concentrations (0, 5, 10, and 20 μM). IC50 is determined with the assay data[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice: Tumors were established in mice for 5 days prior to oral or intraperitoneal treatments with MT-DADMe-ImmA. Mice are treated with oral dose of 21 mg/kg or an intraperitoneal dose of 5 mg/kg/day MT-DADMe-ImmA[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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MT-DADMe-ImmA
Cat. No.:
HY-101496
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