NKL 22
Based on 1 publication(s) in Google Scholar
NKL 22 is a potent and selective inhibitor of histone deacetylases (HDAC), with IC50 values of 199 and 69 nM for HDAC1 and HDAC3, respectively. NKL 22 can reverse abnormal expression of HD‑related genes and restore the levels of key genes including Ppp1r1b in Huntington's disease transgenic mice. NKL 22 can be used for the researches of Huntington's disease and cancer.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 97.26%
- CAS No.: 537034-15-4
- 화학식: C19H23N3O2
- 분자량:325.40
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) NKL 22
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Biological Activity
제품 설명
IC50 & Target
[2]|
HDAC3 0.069 μM (IC50) |
HDAC1 0.199 μM (IC50) |
HDAC2 1.59 μM (IC50) |
HDAC8 5 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
78 μM
Compound: 4b
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Inhibition of HDAC in human HeLa cell extract by fluorescence plate reader assay
Inhibition of HDAC in human HeLa cell extract by fluorescence plate reader assay
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[PMID: 16921367] |
In Vitro
NKL 22 (Compound 4b) shows IC50 values of 199 and 69 nM for HDAC1 and HDAC3, respectively, and shows IC50 values of 1.59 and 5 μM for HDAC4/8 >180 μM for HDAC4/5/7[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:R6/2/N171-82Q Huntington’s disease transgenic mice[2]
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Dosage:150 mg/kg
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Administration:s.c., once daily for 3 consecutive days
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Result:Reversed abnormal expression of HD‑related genes and restored the levels of key genes including Ppp1r1b.
Ameliorated motor and behavioral phenotypes.
Did not alter the total expression of HDAC1/3 but promotes their nuclear accumulation in N171-82Q Huntington’s disease transgenic mice.
Chemical Information
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CAS No. 537034-15-4
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Appearance Solid
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분자량 325.40
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화학식 C19H23N3O2
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Color White to off-white
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SMILES
O=C(NC1=CC=CC=C1N)CCCCCC(NC2=CC=CC=C2)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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J Mol Med (Berl)
2019 Aug;97(8):1183-1193. PMID: 31201471
용액&용해도
In Vitro:
DMSO : 100 mg/mL (307.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
순도&문서
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. He S, et al. Histone Deacetylase Inhibitor Entinostat Exerts Anti-NSCLC Effects Through the EGFR Signaling Pathway and MDM2-p53 Axis. Curr Pharm Biotechnol. Published online January 5, 2026. [Content Brief]
[2]. Jia H, et al. Histone deacetylase (HDAC) inhibitors targeting HDAC3 and HDAC1 ameliorate polyglutamine-elicited phenotypes in model systems of Huntington's disease. Neurobiol Dis. 2012;46(2):351-361. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0731 mL | 15.3657 mL | 30.7314 mL | 76.8285 mL |
| 5 mM | 0.6146 mL | 3.0731 mL | 6.1463 mL | 15.3657 mL | |
| 10 mM | 0.3073 mL | 1.5366 mL | 3.0731 mL | 7.6829 mL | |
| 15 mM | 0.2049 mL | 1.0244 mL | 2.0488 mL | 5.1219 mL | |
| 20 mM | 0.1537 mL | 0.7683 mL | 1.5366 mL | 3.8414 mL | |
| 25 mM | 0.1229 mL | 0.6146 mL | 1.2293 mL | 3.0731 mL | |
| 30 mM | 0.1024 mL | 0.5122 mL | 1.0244 mL | 2.5610 mL | |
| 40 mM | 0.0768 mL | 0.3841 mL | 0.7683 mL | 1.9207 mL | |
| 50 mM | 0.0615 mL | 0.3073 mL | 0.6146 mL | 1.5366 mL | |
| 60 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2805 mL | |
| 80 mM | 0.0384 mL | 0.1921 mL | 0.3841 mL | 0.9604 mL | |
| 100 mM | 0.0307 mL | 0.1537 mL | 0.3073 mL | 0.7683 mL |
NKL 22 Related Classifications
HY-100384 Related Classifications
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