Fluorescent Dye
Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.
Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.
Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.
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Fluorescent Dye Related Products (4914)
Related Products (4914)
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Antibodies (4)
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Aldehyde reactive probe TFA
0 ImagesCat. No.: HY-135136CAS No.: 627090-10-2Aldehyde reactive probe TFA is an aldehyde reactive probe. Aldehyde reactive probe TFA specifically labels the AP site in DNA with biotin residues. Aldehyde reactive probe TFA can detect RNA oxidation-induced debasement sites. Aldehyde reactive probe TFA can be used to quantify AP sites or detect other aldehyde-containing DNA damage and RNA oxidation-induced debasement sites.
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Lumiflavin
0 ImagesLumiflavin (Lumiflavine), a riboflavine analog, causes significant inhibition of riboflavine uptake. Lumiflavin can effectively reduce the riboflavin enrichment in cancer stem-like cells (CSCs) and sensitize the effect of cisplatin Diamminedichloroplatinum (DDP) on CSCs. Lumiflavin is promising for research of ovarian cancer.
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BODIPY-FL NHS ester
0 ImagesSynonyms: BODIPY FL,SEBODIPY FL NHS ester (BODIPY FL, SE) is a cell membranes-penatrable amine-reactive fluorescent probe. The maximum excitation/emission wavelength of the BODIPY-FL NHS ester are 502/511 nm, respectively. BODIPY-FL NHS ester has high stability and is insensitive to the polarity, pH and type of solvent, and can maintain stable fluorescence properties under different environmental conditions. BODIPY-FL NHS ester can be used for the synthesis of protease substrates, live cell imaging, protein labeling and immunoassay.
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Fluoresterol
0 ImagesSynonyms: 22-NBD CholesterolFluoresterol (22-NBD Cholesterol) is a cholesterol-specific fluorescent probe with cholesterol-mimicking binding properties. Fluoresterol is ineffective orally and does not cross the blood-brain barrier. Fluoresterol specifically binds to cholesterol transport-related proteins (such as ABCA1 and ABCG1) and is primarily used in cholesterol metabolism research, particularly for the visualization and quantitative analysis of cholesterol absorption, efflux, intracellular transport efficiency, and reverse cholesterol transport (RCT) processes. The commonly used concentration of Fluoresterol in in vitro experiments is 0.1-10 μM, and the commonly used dose in in vivo experiments is 5-20 mg/kg (gavage or intraperitoneal injection), with excitation/emission wavelengths of 472/540 nm. Fluoresterol can be applied to the study of cholesterol metabolism mechanisms related to hyperlipidemia, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD).
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Di-4-ANEPPDHQ
0 ImagesDi-4-ANEPPDHQ is a polarity-sensitive membrane probe with a doubly positive charge that can be used to visualize membrane microdomains in living Arabidopsis cells. Di-4-ANEPPDHQ produces a fluorescence spectrum shift by changing the polarity of the lipid environment after embedding into the cell membrane, thereby quantifying the degree of lipid accumulation. The excitation wavelength of Di-4-ANEPPDHQ is 488 nm, and the emission wavelengths are 560 nm and 650 nm in the liquid ordered and disordered phases, respectively. Di-4-ANEPPDHQ is often used for fluorescence imaging studies of membrane microstructure, lipid order, and membrane tension response in living cells[1][2][3].
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NBD-Cl
0 ImagesSynonyms: NBD chlorideNBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.
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Cy3-dCTP
0 ImagesCat. No.: HY-D1725Purity: 98.70%Cy3-dCTP is a directly fluorescently labeled deoxyribonucleotide, in which Cy3 is a cyanine fluorescent dye. Cy3-dCTP is used for direct enzymatic labeling of DNA and cDNA: with the aid of DNA polymerases, this modified nucleotide is incorporated into the extending DNA strand during processes such as reverse transcription, PCR, nick translation or random primer labeling.
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Peroxy Orange 1
0 ImagesSynonyms: PO1Peroxy Orange 1 (PO1) is a new H2O2 specific probe that can bind to green fluorescent highly reactive oxygen species (hROS) probe APF. Peroxy Orange 1 is also a living cell dye.
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DCLK1-IN-1
0 ImagesDCLK1-IN-1 is a selective, oral bioavailability in vivo-compatible chemical probe of the doublecortin like kinase 1 (DCLK1 kinase) domain. DCLK1-IN-1 inhibits DCLK1 and DCLK2 kinases (IC50: DCLK1=9.5/57.2 nM and DCLK2=31/103 nM in binding and kinase assay, respectively). DCLK1-IN-1 shows low toxicity, and can investigate DCLK1 biology and establish its role in cancer, like DCLK1+ pancreatic ductal adenocarcinoma (PDAC).
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BCECF
0 ImagesCat. No.: HY-101882CAS No.: 85138-49-4Synonyms: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluoresceinBCECF is a cell-impermeant pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
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1,8-Diazafluoren-9-one
0 ImagesSynonyms: DFO1,8-Diazafluoren-9-one (DFO) is a chemical that is used to find fingerprints on porous surfaces. 1,8-Diazafluoren-9-one reacts with amino acids present in the fingerprint to form highly fluorescent derivatives (Ex/Em = ~470/570).
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5-TAMRA
0 Images5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability. 5-TAMRA is mainly used as a fluorescent marker for the synthesis and study of specific oligonucleotide probes.
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NBD-PE
0 ImagesNBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology.
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Zinquin ethyl ester
0 ImagesZinquin ethyl ester is a cell-permeable and lipophilic fluorescent derivative of Zinquin (HY-D0982). Zinquin ethyl ester reacts with protein-bound Zn2+ in cells and forms fluorescent ternary adducts. Zinquin ethyl ester undergoes hydrolysis by intracellular esterases impeding its efflux across the plasma membrane (Ex/Em = 370/470 nm).
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DFHO
0 ImagesDFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells.
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Bromophenol blue
0 ImagesBromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.
