Art. -Nr.: HY-101920

Autophinib

12 Cited Publications

Kundenbewertung

Based on 12 publication(s) in Google Scholar

Autophinib is a potent, selective autophagy inhibitor with IC₅₀s of 90 nM and 40 nM for starvation- and Rapamycin-induced autophagy, respectively. Autophinib is also an ATP competitive Vacuolar Protein Sorting 34 (VPS34) inhibitor with an IC₅₀ of 19 nM. Autophinib inhibits autophagy induced by starvation or Rapamycin by targeting VPS34.

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Reinheit: 99.95%
CAS. Nr.: 1644443-47-9
Formel: C₁₄H₁₁ClN₆O₃
Molecular Weight:346.73

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Autophinib Chemical Structure

CAS. Nr.: 1644443-47-9

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (12)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

Vps34

19 nM (IC₅₀)

Alle PI3K Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	0.04 μM Compound: 45	Inhibition of rapamycin induced autophagy in human MCF7 cells expressing eGFP-LC3 Inhibition of rapamycin induced autophagy in human MCF7 cells expressing eGFP-LC3	[PMID: 36283182]
MCF7	IC₅₀	0.09 μM Compound: 45	Inhibition of starvation induced autophagy in human MCF7 cells expressing eGFP-LC3 Inhibition of starvation induced autophagy in human MCF7 cells expressing eGFP-LC3	[PMID: 36283182]

In Vitro

Autophinib (0.01-1 μM) inhibits LC3 lipidation to form LC3-II in a dose-dependent manner in starved MCF7-LC3 cells. Consistent with inhibition of autophagic flux, Autophinib also inhibits p62 degradation by autophagy dose-dependently in MCF7-LC3 cells^[1].
Autophinib enhances cell death (EC₅₀ of 264 nM) of starved cells as compared to fed cells, which occurred via the induction of apoptosis (EC₅₀ of 234 nM) in MCF7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1644443-47-9
Appearance Solid
Molecular Weight 346.73
Formel C₁₄H₁₁ClN₆O₃
Color Light yellow to yellow
SMILES

CC1=CC(NC2=NC(OC3=CC=C([N+]([O-])=O)C=C3)=NC(Cl)=C2)=NN1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (12)

All WB

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Journal Impact Factor

J Extracell Vesicles

Exosomal lipid PI4P regulates small extracellular vesicle secretion by modulating intraluminal vesicle formation. [Abstract]

2023 Apr;12(4):e12319. PMID: 37021404
J Nanobiotechnology

Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivity. [Abstract]

2025 Feb 14;23(1):109. PMID: 39953537
Cell Death Dis

Whole transcriptome and proteome analyses identify potential targets and mechanisms underlying tumor treating fields against glioblastoma. [Abstract]

2022 Aug 18;13(8):721. PMID: 35982032
Cell Death Dis

Increased expression of lncRNA CASC9 promotes tumor progression by suppressing autophagy-mediated cell apoptosis via the AKT/mTOR pathway in oral squamous cell carcinoma. [Abstract]

2019 Jan 17;10(2):41. PMID: 30674868
Redox Rep

The novel thioredoxin reductase inhibitor butaselen suppresses lung cancer by inducing oxidative stress. [Abstract]

2025 Dec;30(1):2588086. PMID: 41292488
Cancer Sci

Loss of the clock gene Per1 promotes oral squamous cell carcinoma progression via the AKT/mTOR pathway. [Abstract]

2020 May;111(5):1542-1554. PMID: 32086839

Autophinib purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2020 May;111(5):1542-1554. [Abstract]

Western blotting shows that the decreased protein expression of P62 in Per1‐OE SCC15 cells is remarkably increased after the addition of Autophinib, while the increased LC3BII/LC3BI ratio and Beclin1 expression are remarkably decreased.
Fish Shellfish Immunol

Butyrate induces STAT3/HIF-1α/IL-22 signaling via GPCR and HDAC3 inhibition to activate autophagy in head kidney macrophages from turbot (Scophthalmus maximus L.). [Abstract]

2023 Dec:143:109214. PMID: 37977544
J Cancer

Overexpression of the clock gene Per2 suppresses oral squamous cell carcinoma progression by activating autophagy via the PI3K/AKT/mTOR pathway. [Abstract]

2020 Mar 26;11(12):3655-3666. PMID: 32284762
Allergy Asthma Clin Immunol

KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation. [Abstract]

2022 Jun 19;18(1):55. PMID: 35718777
Vet Microbiol

Lipid metabolism is a novel and practical source of potential targets for antiviral discovery against porcine parvovirus. [Abstract]

2021 Oct:261:109177. PMID: 34391196
bioRxiv

2025 Sep 21.
Research Square Preprint

2023 Jun 26.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 6.67 mg/mL (19.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8841 mL

14.4204 mL

28.8409 mL

5 mM

0.5768 mL

2.8841 mL

5.7682 mL

10 mM

0.2884 mL

1.4420 mL

2.8841 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 0.5 mg/mL (1.44 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Robke L, et al. Phenotypic Identification of a Novel Autophagy Inhibitor Chemotype Targeting Lipid Kinase VPS34. Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8153-8157. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8841 mL	14.4204 mL	28.8409 mL	72.1022 mL
	5 mM	0.5768 mL	2.8841 mL	5.7682 mL	14.4204 mL
	10 mM	0.2884 mL	1.4420 mL	2.8841 mL	7.2102 mL
	15 mM	0.1923 mL	0.9614 mL	1.9227 mL	4.8068 mL

Keywords

Autophinib

12 Cited Publications

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Autophinib

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Highly Selective Inhibitors Library

Cell Death Library

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Bioactive Compound Library Max

MCE Bioactive Compound Library

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Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Autophinib

Alle PI3K Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (12)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Autophinib Related Classifications

HY-101920 Related Classifications