Art. -Nr.: HY-W063968

RO8191

(Synonyms: CDM-3008; RO4948191)

28 Cited Publications

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Based on 28 publication(s) in Google Scholar

RO8191 (CDM-3008), an imidazonaphthyridine compound, is an orally active and potent interferon (IFN) receptor agonist. RO8191 directly binds to IFNα/β receptor 2 (IFNAR2) and activates IFN-stimulated genes (ISGs) expression and JAK/STAT phosphorylation. RO8191 shows antiviral activity against both HCV and EMCV with an IC₅₀ of 200 nM for HCV replicon. RO8191 is a cccDNA modulator (CDM) through interferon-like activity and has anti-HBV activity.

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Reinheit: 98.80%
CAS. Nr.: 691868-88-9
Formel: C₁₄H₅F₆N₅O
Molecular Weight:373.21

Speicherung:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

RO8191 Chemical Structure

CAS. Nr.: 691868-88-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (28)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

RO8191 (CDM-3008), an imidazonaphthyridine compound, is an orally active and potent interferon (IFN) receptor agonist. RO8191 directly binds to IFNα/β receptor 2 (IFNAR2) and activates IFN-stimulated genes (ISGs) expression and JAK/STAT phosphorylation. RO8191 shows antiviral activity against both HCV and EMCV with an IC₅₀ of 200 nM for HCV replicon^[1]. RO8191 is a cccDNA modulator (CDM) through interferon-like activity and has anti-HBV activity^[2].

IC₅₀ & Target^[1]

JAK1

Alle JAK Isoform-spezifische Produkte anzeigen :

Alle STAT Isoform-spezifische Produkte anzeigen :

In Vitro

RO8191 (CDM-3008) exerts antiviral activity dependent on IFNAR2/JAK1, but is independent of IFNAR1/Tyk2. RO8191, an IFNAR2 agonist, stimulates IFN signals in mice^[1].
RO8191 (0.08, 0.4, 2, 10 μM; for 72 h) strongly suppresses HCV replicon activity at 72 h in a dose-dependent manner. RO8191 reduces levels of the proteins HCV NS3 and NS4A, which are localized mainly in the perinuclear region of the replicon cells^[1].
RO8191 (0.08-10 μM; for 72 h) results in the disappearance of viral proteins such as NS3, NS4A/B, and NS5A/B^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RO8191 (CDM-3008; 30 mg/kg; oral) significantly induces antiviral genes Oas1b, Mx1, and Pkr in the livers of six-week-old C57BL/6J mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 691868-88-9
Appearance Solid
Molecular Weight 373.21
Formel C₁₄H₅F₆N₅O
Color Off-white to yellow
SMILES

FC(C1=C2C=CC3=NC(C4=NN=CO4)=CN3C2=NC(C(F)(F)F)=C1)(F)F
Synonyms

CDM-3008; RO4948191
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (28)

All WBBio/Physico-chemical AssayHistological Imaging/StainingIFFlow Cytometry

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Journal Impact Factor

Acta Biomater

Matrix mechanics regulate the polarization state of bone marrow-derived neutrophils through the JAK1/STAT3 signaling pathway. [Abstract]

2023 Sep 15:168:159-173. PMID: 37467837
Int J Biol Macromol

Lentinan attenuates histone deacetylation of SOCS1 promoter to remodel autophagy via JAK2/STAT3 pathway for the prevention of intervertebral disc degeneration. [Abstract]

2025 Sep;321(Pt 4):146457. PMID: 40749910
Phytomedicine

Oleic acid regulates CD4+ T cells differentiation by targeting ODC1-mediated STAT5A phosphorylation in Vogt-Koyanagi-Harada disease. [Abstract]

2025 Jun:141:156660. PMID: 40203473

RO8191 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156660. [Abstract]

Protein expression and phosphorylation of CD4⁺ T cells treated with BSA, OA or OA+RO8191 (20 μM; 3 days) assessed by western blotting (n = 3 per group). The results showed that addition of RO8191 to OA-treated CD4⁺ T cells significantly reversed the down-regulation of pSTAT5A (Y694) as well as inhibition of IL-10 expression.

RO8191 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156660. [Abstract]

Representative images of p-STAT5A (Y694) in CD4⁺ T cells treated with OA or OA + RO8191 (20 μM; 3 days), with quantification of p-STAT5A (Y694). Immunofluorescence showed that the nucleus localization of pSTAT5A was enhanced after addition of RO8191 in OA-treated CD4⁺ T cells. scale bar, 20 μm.

RO8191 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156660. [Abstract]

Flow cytometry analysis of the percentage of Th1, Th17 and Treg cells in CD4⁺ T cells treated with BSA, OA, or OA+. NS, non-significance. RO8191 (20 μM; 3 days) reversed the up-regulation of Th17 and suppression of Treg cell proportion, but not the promotion of Th1 cell frequency in OA-treated CD4⁺ T cells.
Cancer Immunol Res

Discovery of podofilox as a potent cGAMP-STING signaling enhancer with antitumor activity. [Abstract]

2023 May 3;11(5):583-599. PMID: 36921097

RO8191 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Res. 2023 May 3;11(5):583-599. [Abstract]

ISG-THP1 (WT) or ISG-THP1 (STING KO) cells were treated with cGAMP (0.5 μM), or RO8191 (2.5 μM) with or without various doses of podofilox for 24 h. Normalized values for ISRE luciferase activity induced by cGAMP (or RO8191) along with podofilox are shown in comparison to the activity triggered by DMSO. The results showed that podofilox only showed a tendency to enhance the ISRE-driven immune response when stimulated with RO8191, which is an agonist that directly binds to IFNAR2 and activates the expression of ISGs.
Stem Cell Res Ther

LNGFR promoting osteogenic differentiation of ectomesenchyme stem cells via activation of GHR-JAK-STAT/IGF1 signaling pathway. [Abstract]

2026 May 9;17(1):238. PMID: 42106838
Cell Death Discov

EZH2-STAT3 signaling pathway regulates GSDMD-mediated pyroptosis in glioblastoma. [Abstract]

2024 Jul 28;10(1):341. PMID: 39069522

RO8191 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Jul 28;10(1):341. [Abstract]

RO8191 (30 mg/kg; p.o.; once daily for 14 days). Representative haematoxylin and eosin (H&E) sections of tracheal allografts harvested on day 28 (left panel) and corresponding statistical diagram (right panel) showing the degree of stenosis.

RO8191 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Jul 28;10(1):341. [Abstract]

RO8191 (30 mg/kg; p.o.; once daily for 14 days). Representative Masson staining sections of the transplanted trachea (left panel) and corresponding statistical charts (right panel) illustrating collagen deposition.
Clin Transl Med

cGAS/STING signalling in macrophages aggravates obliterative bronchiolitis via an IFN-α-dependent mechanism after orthotopic tracheal transplantation in mice. [Abstract]

2025 May;15(5):e70323. PMID: 40292672
Phytother Res

Curcumin Alleviates Bone Cancer Pain by Inhibiting Satellite Glial Cell Activity via Janus Kinase 1/Signal Transducer and Transcription 3 Pathway Activator. [Abstract]

2026 Jul;40(7):4346-4366. PMID: 42087052
Microb Biotechnol

Akkermansia muciniphila outer membrane protein regulates recruitment of CD8+ T cells in lung adenocarcinoma and through JAK-STAT signalling pathway. [Abstract]

2024 Jul;17(7):e14522. PMID: 39016683
Int Immunopharmacol

The JAK1/STAT3 pathway mediates the effects of SERPINH1 on glioma EMT. [Abstract]

2025 May 6:157:114731. PMID: 40334628
Int Immunopharmacol

L-Kynurenine regulates immune response in ICIs-associated myocarditis via JAK/STAT pathway. [Abstract]

2025 Apr 22:156:114676. PMID: 40267722
Reprod Biol Endocrinol

Liraglutide improves follicle development in polycystic ovary syndrome by inhibiting CXCL10 secretion. [Abstract]

2024 Aug 6;22(1):98. PMID: 39107809
Int Immunopharmacol

Obovatol inhibits proliferation, invasion and immune escape of hepatocellular carcinoma cells through modulating the JAK/STST3/PD-L1 pathway. [Abstract]

2024 Aug 14:141:112775. PMID: 39146776
Poult Sci

Up-regulated Lnc BTU promotes the production of duck plague virus DNA polymerase and inhibits the activation of JAK-STAT pathway to facilitate duck plague virus replication. [Abstract]

2024 Dec;103(12):104238. PMID: 39383668
J Cell Mol Med

MTHFD2 promotes PD-L1 expression via activation of the JAK/STAT signalling pathway in bladder cancer. [Abstract]

2023 Oct;27(19):2922-2936. PMID: 37480214
Oncol Rep

Synergistic effects of thalidomide and cisplatin are mediated via the PI3K/AKT and JAK1/STAT3 signaling pathways in cervical cancer. [Abstract]

2022 Oct;48(4):169. PMID: 35920185
Mol Med Rep

Fucoxanthin alleviates chondrocyte inflammation and osteoarthritis by modulating the JAK2/STAT3 signaling pathway. [Abstract]

2026 Aug;34(2):223. PMID: 42272246
Appl Biochem Biotechnol

DUSP7 Overexpression Suppresses Lung Adenocarcinoma Cell Proliferation, Invasion, and M2 Macrophage Polarization Through JAK2/STAT3 Pathway Inhibition. [Abstract]

2026 Feb;198(2):1337-1356. PMID: 41484728
Curr Issues Mol Biol

Impact of SARS-CoV-2 Variant NSP6 on Pathogenicity: Genetic Analysis and Cell Biology. [Abstract]

2025 May 14;47(5):361. PMID: 40699760
J Biochem Mol Toxicol

Falcarindiol Suppresses Malignant Progression and Induces Ferroptosis in Non-Small Cell Lung Cancer by Regulating JAK/STAT3 Axis. [Abstract]

2025 Sep;39(9):e70486. PMID: 40901755
Theriogenology

LncRNA STAT3-AS regulates endometrial receptivity via the STAT3 signaling pathway. [Abstract]

2024 Mar 1:216:118-126. PMID: 38171198
BMC Musculoskelet Disord

Knockdown of CLEC4D alleviates myoblast dysfunction and inflammation in sarcopenia by inhibiting the JAK/STAT pathway. [Abstract]

2025 Oct 21;26(1):981. PMID: 41121007
Am J Reprod Immunol

NANOG regulate the JAK/STAT3 pathway to promote trophoblast cell migration and epithelial-mesenchymal transition (EMT) in hypertensive disorders of pregnancy (HDP) through protein interaction with CDK1. [Abstract]

2024 May;91(5):e13863. PMID: 38796740
Virology

Avian coronavirus infectious bronchitis virus Beaudette strain NSP9 interacts with STAT1 and inhibits its phosphorylation to facilitate viral replication. [Abstract]

2024 Feb:590:109944. PMID: 38141500
Vet Sci

CD4 Molecule Plays an Important Role in the Inflammatory Response Induced by Japanese Encephalitis Virus Infection. [Abstract]

2026 Mar 9;13(3):254. PMID: 41893671
Graefes Arch Clin Exp Ophthalmol

FoxO1 promotes high glucose-induced inflammation and cataract formation via JAK1/STAT1. [Abstract]

2025 Jun;263(6):1585-1596. PMID: 39878886
J Mol Histol

FAM64A regulates the malignant phenotype and tumor microenvironment of non-small cell lung cancer by activating the JAK/STAT3/PDL1 axis. [Abstract]

2025 Feb 24;56(2):95. PMID: 39992461
Res Sq

2026 Jun 1.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 5 mg/mL (13.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6795 mL

13.3973 mL

26.7946 mL

5 mM

0.5359 mL

2.6795 mL

5.3589 mL

10 mM

0.2679 mL

1.3397 mL

2.6795 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 0.5 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 0.5 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (1.34 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Hideyuki Konishi, et al. An Orally Available, Small-Molecule Interferon Inhibits Viral Replication. Sci Rep. 2012;2:259. [Content Brief]

[2]. Yutaka Furutani, et al. An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes. PLoS One. 2019 Jun 12;14(6):e0216139. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6795 mL	13.3973 mL	26.7946 mL	66.9864 mL
	5 mM	0.5359 mL	2.6795 mL	5.3589 mL	13.3973 mL
	10 mM	0.2679 mL	1.3397 mL	2.6795 mL	6.6986 mL

RO8191

28 Cited Publications

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RO8191

Publications Citing Use of MedChemExpress (MCE) RO8191

Alle JAK Isoform-spezifische Produkte anzeigen

Alle STAT Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (28)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RO8191 Related Classifications

HY-W063968 Related Classifications