2′-Deoxy-ADPR

2′-deoxy-ADPR is generated by hydrolysis of 2′-deoxy-NAD by recombinant human CD38^[1].
2′-deoxy-ADPR is a highly potent TRPM2 agonist in HEK293 cells expressing hTRPM2, producing whole-cell currents 10.4-fold higher than ADPR, with maximal currents of 14.3-16.7 nA under 50-200 nM free Ca²⁺ conditions^[1].
2′-deoxy-ADPR increases the open probability to a median of 0.92 in excised inside-out membrane patches from HEK293 cells expressing hTRPM2, and slows inactivation, but does not alter single-channel conductance^[1].
2′-deoxy-ADPR is endogenously present in Jurkat T cells at 35 pmol per 10⁷ cells (approximately 9.8 µM in the cytosol), and H₂O₂ increases it 2.71-fold to 95 pmol per 10⁷ cells^[1].
2′-Deoxy-ADPR (2dADPR) (100 μM) activates hsTRPM2 in HEK293 cells with an EC₅₀ of 192 nM; at intracellular Ca²⁺ concentrations below 1 μM, it activates the channel faster than ADPR in HEK293 cells expressing hsTRPM2^[2].
In transfected HEK293 cells, the W1355A mutation in the NUDT9H domain of 2′-Deoxy-ADPR eliminates the difference in activation rate between it and ADPR^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.