LY3177833
Based on 1 Customer Validation
LY3177833 (Example 4) is an orally active CDC7 and pMCM2 inhibitor with IC50 values of 3.3 nM and 290 nM, respectively. LY3177833 is a senescence inducer.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.56%
- CAS No.: 1627696-51-8
- Formule: C16H12FN5O
- Masse moléculaire:309.30
-
Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
-
HY-100888
SimurosertibCDC7 Inhibitor -
HY-15260A
XL413 monohydrochlorideCDC7 Inhibitor -
HY-100888R
Simurosertib (Standard)CDC7 Inhibitor -
HY-15260
XL413CDC7 Inhibitor -
HY-15260C
XL413 hydrochlorideCDC7 Inhibitor -
HY-A0065
Palbociclib isethionateCDK4/6 Inhibitor -
HY-18930
NU6300CDK2 Inhibitor -
HY-15777A
Ribociclib hydrochlorideCDK4/6 Inhibitor -
HY-15777B
Ribociclib succinateCDK4/6 Inhibitor -
HY-101467A
Trilaciclib hydrochlorideCDK4/6 Inhibitor -
HY-112272A
Lerociclib dihydrochlorideCDK4/CDK6 Inhibitor -
HY-112822
ON-013100CDK Inhibitor
Activité biologique
Description
IC50 & Target
[1]|
Cdc7 3.3 nM (IC50) |
pMCM2 290 nM (IC50) |
In Vitro
LY3177833 (10 μM; 4 days) increases SA-β-gal content in Hep3B cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Hep3B cells
-
Concentration:10 μM
-
Incubation Time:4 days
-
Result:Increased the expression of human SA-β-gal.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Female athymic Balb/c nude mice (5-6 weeks old) with SW620 cells[1]
-
Dosage:10.4 mg/kg, 20.8 mg/kg and 31.2 mg/kg
-
Administration:Oral gavage; twice a day for 2 weeks
-
Result:Showed dose dependent antitumor activity in SW620 mouse xenograft tumor model.
Chemical Information
-
CAS No. 1627696-51-8
-
Appearance Solid
-
Masse moléculaire 309.30
-
Formule C16H12FN5O
-
Color White to off-white
-
SMILES
O=C1N[C@@](C)(C2=NC=NC=C2F)C3=C1C=C(C4=CNN=C4)C=C3
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvant et solubilité
In Vitro:
DMSO : ≥ 30 mg/mL (96.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
-
Fiche technique (279 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2331 mL | 16.1655 mL | 32.3311 mL | 80.8277 mL |
| 5 mM | 0.6466 mL | 3.2331 mL | 6.4662 mL | 16.1655 mL | |
| 10 mM | 0.3233 mL | 1.6166 mL | 3.2331 mL | 8.0828 mL | |
| 15 mM | 0.2155 mL | 1.0777 mL | 2.1554 mL | 5.3885 mL | |
| 20 mM | 0.1617 mL | 0.8083 mL | 1.6166 mL | 4.0414 mL | |
| 25 mM | 0.1293 mL | 0.6466 mL | 1.2932 mL | 3.2331 mL | |
| 30 mM | 0.1078 mL | 0.5389 mL | 1.0777 mL | 2.6943 mL | |
| 40 mM | 0.0808 mL | 0.4041 mL | 0.8083 mL | 2.0207 mL | |
| 50 mM | 0.0647 mL | 0.3233 mL | 0.6466 mL | 1.6166 mL | |
| 60 mM | 0.0539 mL | 0.2694 mL | 0.5389 mL | 1.3471 mL | |
| 80 mM | 0.0404 mL | 0.2021 mL | 0.4041 mL | 1.0103 mL |
LY3177833 Related Classifications
HY-100023 Related Classifications
Powered by Bioz