Carbobenzoxyproline (Synonyms: L-Cbz-Proline)

Cat. No.: HY-Y0588 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

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Carbobenzoxyproline (L-Cbz-Proline) is an inhibitor of prolidase. Carbobenzoxyproline can be used for prolidase deficiency (PD) research.

For research use only. We do not sell to patients.

Carbobenzoxyproline Chemical Structure

CAS No. : 1148-11-4

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10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
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100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Description

Carbobenzoxyproline (L-Cbz-Proline) is an inhibitor of prolidase. Carbobenzoxyproline can be used for prolidase deficiency (PD) research^[1].

In Vitro

Carbobenzoxyproline (6 mM; 0-10 d; pH=6.0) causes mitochondrial depolarization and increases cellular death by 33% as reported for long-term culture of fibroblasts from prolidase deficiency (PD) patients^[1].
Carbobenzoxyproline (0, 1, 3, 6 mM; 1 min; pH=6.0) results fibroblasts prolidase (FBP) hydrolysis, shows linear competitive inhibition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Carbobenzoxyproline (60 mg/kg; injection; once daily; 3 weeks) serves as in vivo inhibitor of erythrocytes prolidase in mice model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6J mice (4-week-old)^[1]
Dosage:	60 mg/kg
Administration:	Injection; once daily for 3 weeks
Result:	Resulted significant reduction of erythrocytes prolidase activity.

Molecular Weight

249.26

Formula

C₁₃H₁₅NO₄

CAS No.

1148-11-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1[C@H](C(O)=O)CCC1)OCC2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (401.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0119 mL	20.0594 mL	40.1188 mL
5 mM	0.8024 mL	4.0119 mL	8.0238 mL
10 mM	0.4012 mL	2.0059 mL	4.0119 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (252 KB) HNMR (119 KB) RP-HPLC (177 KB) NP-HPLC (448 KB) OR (82 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0119 mL	20.0594 mL	40.1188 mL	100.2969 mL
	5 mM	0.8024 mL	4.0119 mL	8.0238 mL	20.0594 mL
	10 mM	0.4012 mL	2.0059 mL	4.0119 mL	10.0297 mL
	15 mM	0.2675 mL	1.3373 mL	2.6746 mL	6.6865 mL
	20 mM	0.2006 mL	1.0030 mL	2.0059 mL	5.0148 mL
	25 mM	0.1605 mL	0.8024 mL	1.6048 mL	4.0119 mL
	30 mM	0.1337 mL	0.6686 mL	1.3373 mL	3.3432 mL
	40 mM	0.1003 mL	0.5015 mL	1.0030 mL	2.5074 mL
	50 mM	0.0802 mL	0.4012 mL	0.8024 mL	2.0059 mL
	60 mM	0.0669 mL	0.3343 mL	0.6686 mL	1.6716 mL
	80 mM	0.0501 mL	0.2507 mL	0.5015 mL	1.2537 mL
	100 mM	0.0401 mL	0.2006 mL	0.4012 mL	1.0030 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Carbobenzoxyproline1148-11-4L-Cbz-ProlineInhibitorinhibitorinhibit

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