IA-Alkyne
Based on 4 publication(s) in Google Scholar
IA-Alkyne (Iodoacetamide-alkyne; N-Hex-5-ynyl-2-iodo-acetamide) is a TRP channel (TRPC) agonist and has the potential for the study of respiratory infection. IA-Alkyne can be used to develop an isotopically tagged probe for quantitative cysteine-reactivity profiling. IA-Alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.59%
- CAS. Nr.: 930800-38-7
- Formel: C8H12INO
- Molecular Weight:265.09
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Anti-Infection Compound Library
HY-L002
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Alkyne Compound Library
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Publications Citing Use of MedChemExpress (MCE) IA-Alkyne
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Customer Validation & Images
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WB
Biologische Aktivität
IC50: TRPC[1]
IA-alkyne (2.5-10 μM; 1 hour) can react with cysteines. Soluble fractions of HeLa cell lysates (2.0 mg/ml) are treated with IA-alkyne. Labeled proteome samples are conjugated with a fluorescent rhodamine-azide tag through CuAAC, and analyzed by SDS-PAGE. In-gel fluorescence imaging reveals that IA-alkyne has a strong fluorescent intensity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 930800-38-7
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Appearance Solid
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Molecular Weight 265.09
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Formel C8H12INO
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Color White to off-white
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SMILES
O=C(NCCCCC#C)CI
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Synonyms
Iodoacetamide-alkyne; N-Hex-5-ynyl-2-iodo-acetamide
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Acta Pharmacol Sin
Triptolide derivative STP1 ameliorates murine systemic lupus erythematosus via targeting Fyn kinase. [Abstract]2026 Feb 11. PMID: 41673457
IA-Alkyne purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2026 Feb 11. [Abstract]
Fyn was validated as a direct target of STP1 in splenocyte lysate by ABPP and Western blotting. The results showed that Fyn was obviously pulled down by the azido-iodoacetamide (IA-Alkyne, 10 μM; 1 h) probe, and the amount of Fyn was significantly reduced by STP1 competition.
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Cell Rep
Small-molecule enhancement of METTL3 S-palmitoylation as a therapeutic strategy for osteoarthritis. [Abstract]2026 Feb 18;45(3):116993. PMID: 41719123 -
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Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (377.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (270 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7723 mL | 18.8615 mL | 37.7230 mL | 94.3076 mL |
| 5 mM | 0.7545 mL | 3.7723 mL | 7.5446 mL | 18.8615 mL | |
| 10 mM | 0.3772 mL | 1.8862 mL | 3.7723 mL | 9.4308 mL | |
| 15 mM | 0.2515 mL | 1.2574 mL | 2.5149 mL | 6.2872 mL | |
| 20 mM | 0.1886 mL | 0.9431 mL | 1.8862 mL | 4.7154 mL | |
| 25 mM | 0.1509 mL | 0.7545 mL | 1.5089 mL | 3.7723 mL | |
| 30 mM | 0.1257 mL | 0.6287 mL | 1.2574 mL | 3.1436 mL | |
| 40 mM | 0.0943 mL | 0.4715 mL | 0.9431 mL | 2.3577 mL | |
| 50 mM | 0.0754 mL | 0.3772 mL | 0.7545 mL | 1.8862 mL | |
| 60 mM | 0.0629 mL | 0.3144 mL | 0.6287 mL | 1.5718 mL | |
| 80 mM | 0.0472 mL | 0.2358 mL | 0.4715 mL | 1.1788 mL | |
| 100 mM | 0.0377 mL | 0.1886 mL | 0.3772 mL | 0.9431 mL |
IA-Alkyne Related Classifications
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