Chikusetsusaponin IVa methyl ester  (Synonyms: CME)

Chikusetsusaponin IVa methyl ester (CME) is a natural triterpenoid saponin compound. Chikusetsusaponin IVa methyl ester induces G0/G1 cell cycle arrest and apoptosis in colon cancer cells by inhibiting the Wnt/β-catenin signaling pathway. By inhibiting the NF-κB and AP-1 signaling pathways, Chikusetsusaponin IVa methyl ester significantly reduces the production of NO, PGE₂ and pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), and downregulates the levels of iNOS and COX-2. Chikusetsusaponin IVa methyl ester can be used in researches on colorectal cancer and inflammation^[1][2].

Chikusetsusaponin IVa methyl ester (3-30 μM；7-25 h) concentration-dependently inhibits LPS-induced pro-inflammatory mediator production and gene expression in RAW264.7 macrophages via suppression of iNOS, COX-2, TNF-α, IL-6, and IL-1β expression^[1].
Chikusetsusaponin IVa methyl ester (3-30 μM; 1 h pretreatment, 15-45 min/18 h LPS treatment) concentration-dependently inhibits LPS-induced NF-κB and AP-1 activation in RAW264.7 macrophages via suppression of NF-κB p65 nuclear translocation and c-Fos expression/phosphorylation^[1].
Chikusetsusaponin IVa methyl ester (12.5-100 μM; 24 h) inhibits cell proliferation in HCT116 cells with decreased viability at 25 μM and severe cell death above 50 μM^[2].
Chikusetsusaponin IVa methyl ester (10-40 μM) induces G0/G1 cell cycle arrest in HCT116 cells^[2].
Chikusetsusaponin IVa methyl ester (20-30 μM; 4-24 h) downregulates cyclin D1, CDK2, and CDK4 and upregulates p21 in HCT116 cells in vitro in a time- and dose-dependent manner^[2].
Chikusetsusaponin IVa methyl ester (20-30 μM; 24 h) reduces β-catenin binding to TBE in HCT116 cells in a dose-dependent manner^[2].
Chikusetsusaponin IVa methyl ester (20-30 μM; 24 h) reduces nuclear β-catenin levels in HCT116 cells in a dose-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

808.99

C₄₃H₆₈O₁₄

58546-61-5

OC[C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC([C@]23[C@](CC(C)(CC3)C)([H])C4=CC[C@@]([C@@]5([C@@](C(C)([C@H](CC5)O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O)O)O)C(OC)=O)C)([H])CC7)C)([H])[C@]7(C)[C@@]4(CC2)C)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lee HJ, et al. Chikusetsusaponin IVa Methyl Ester Isolated from the Roots of Achyranthes japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression by NF-κB and AP-1 Inactivation. Biol Pharm Bull. 2016;39(5):657-664.  [Content Brief]

  [2]. Lee KM, et al. Chikusetsusaponin IVa methyl ester induces cell cycle arrest by the inhibition of nuclear translocation of β-catenin in HCT116 cells. Biochem Biophys Res Commun. 2015;459(4):591-596.  [Content Brief]