Cilinaphthalide B
Cilinaphthalide B is an integrin αIIbβ3 inhibitor and a phthalide derivative that inhibits platelet aggregation by binding to integrin αIIbβ3 and interfering with the conformational changes required for its activation, and it exhibits cytotoxicity against cancer cell lines. Cilinaphthalide B can be used in antiplatelet and anticancer-related research.
For research use only. We do not sell to patients.
- CAS No.: 102040-06-2
- Formula: C23H22O7
- Molecular Weight:410.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
αIIbβ3 |
In Vitro
Cilinaphthalide B interacts with integrin αIIbβ3 protein with a binding free energy of −4.575 kcal/mol, forming a π-π stacking with PHE160[1].
Cilinaphthalide B (3 min) exhibits an antiplatelet effect on human platelet-rich plasma[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 102040-06-2
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Molecular Weight 410.42
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Formula C23H22O7
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SMILES
O=C1OCC=2C(OC)=C3C=C(OC)C(OC)=CC3=C(C=4C=CC(OC)=C(OC)C4)C12
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cilinaphthalide B Related Classifications
HY-N21713 Related Classifications
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