2. Stem Cell/Wnt TGF-beta/Smad PI3K/Akt/mTOR Apoptosis
  3. TGF-beta/Smad PI3K Apoptosis
  4. Disitertide diammonium

Disitertide diammonium  (Synonyms: P144 diammonium)

Cat. No.: HY-P0118B Purity: 99.53%
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Disitertide (P144) diammonium is a peptidic transforming growth factor-beta 1 (TGF-β1) inhibitor specifically designed to block the interaction with its receptor. Disitertide diammonium is also a PI3K inhibitor and an apoptosis inducer.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Disitertide diammonium Chemical Structure

Disitertide diammonium Chemical Structure

Size Price Stock Quantity
1 mg USD 115 In-stock
5 mg USD 350 In-stock
10 mg USD 560 In-stock
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Based on 12 publication(s) in Google Scholar

Other Forms of Disitertide diammonium:

Disitertide diammonium Related Antibodies

Top Publications Citing Use of Products

    Disitertide diammonium purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2021 Jan;28(1):219-232.  [Abstract]

    LINC00941-overexpressing cells treated with Disitertide (10 μM) shows increased mRNA and protein levels of ZO-1 and Ecadherin compared with controls. LINC00941-silenced cells show decreased mRNA and protein levels of ZO-1 and E-cadherin but increased mRNA and protein levels of Vimentin, Fibronectin, and Twist1.

    View All PI3K Isoform Specific Products:

    View All Isoforms
    PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Disitertide (P144) diammonium is a peptidic transforming growth factor-beta 1 (TGF-β1) inhibitor specifically designed to block the interaction with its receptor. Disitertide diammonium is also a PI3K inhibitor and an apoptosis inducer[1][2][3][4][5].

    In Vitro

    Disitertide (P144, 100 μg/mL) diammonium suppresses the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells[2].
    Disitertide (TGF-β1 inhibitor) diammonium abrogates the MACC1- AS1 expression in GC cells, suggesting that targeting TGFβ signaling pathway may be a potential strategy to inhibit MSC-induced stemness and chemoresistance[3].
    Disitertide (10 μg/mL to 200 μg/mL) diammonium affects proliferation, induces apoptosis as well as anoikis in A172 and U-87 MG GBM cell lines[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Disitertide diammonium Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Mouse embryo osteoblast precursor MC3T3-E1 cells.
    Concentration: 100 μg/mL
    Incubation Time: 4 h
    Result: Significantly suppressed the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells compared with the miR-590 group.
    In Vivo

    Disitertide (P144, Topical application, 300 μg/mL) diammonium may promote scar maturation and improvement of hypertrophic scar morphology features in an “in vivo” model in nude mice after two weeks of treatment[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Human hypertrophic scars were implanted in 60 nude mice[4].
    Dosage: 300 μg/mL was added the Lipogel.
    Administration: Topical application daily administered.
    Result: Successful shedding was achieved in 83.3% of the xenografts.
    Clinical Trial
    Molecular Weight

    1614.88

    Formula

    C68H115N19O22S2
    Appearance

    Solid

    Color

    White to off-white

    Sequence Shortening

    TSLDASIIWAMMQN
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (6.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 9.09 mg/mL (5.63 mM; ultrasonic and adjust pH to 9 with NH3·H2O)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.6192 mL 3.0962 mL 6.1924 mL
    5 mM 0.1238 mL 0.6192 mL 1.2385 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 5 mg/mL (3.10 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.53%

    COA (242 KB) LCMS (96 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.6192 mL 3.0962 mL 6.1924 mL 15.4810 mL
    5 mM 0.1238 mL 0.6192 mL 1.2385 mL 3.0962 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Disitertide diammonium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Disitertide diammoniumP144 diammoniumTGF-beta/SmadPI3KApoptosisTransforming growth factor betaPhosphoinositide 3-kinasetransforming growth factorpolypeptideGC cellsInhibitorinhibitorinhibit

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