1. Stem Cell/Wnt
    TGF-beta/Smad
    PI3K/Akt/mTOR
    Apoptosis
  2. TGF-beta/Smad
    PI3K
    Apoptosis
  3. Disitertide TFA

Disitertide TFA (Synonyms: P144 TFA)

Cat. No.: HY-P0118A Purity: 96.40%
Handling Instructions

Disitertide (P144) TFA is a peptidic transforming growth factor-beta 1 (TGF-β1) inhibitor specifically designed to block the interaction with its receptor. Disitertide (P144) TFA is also a PI3K inhibitor and an apoptosis inducer.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Disitertide TFA Chemical Structure

Disitertide TFA Chemical Structure

Size Price Stock Quantity
1 mg USD 200 In-stock
Estimated Time of Arrival: December 31
5 mg USD 550 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Disitertide TFA:

Top Publications Citing Use of Products

    Disitertide TFA purchased from MCE. Usage Cited in: Cell Death Differ. 2021 Jan;28(1):219-232.

    LINC00941-overexpressing cells treated with Disitertide (10 μM) shows increased mRNA and protein levels of ZO-1 and Ecadherin compared with controls. LINC00941-silenced cells show decreased mRNA and protein levels of ZO-1 and E-cadherin but increased mRNA and protein levels of Vimentin, Fibronectin, and Twist1.
    • Biological Activity

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    Description

    Disitertide (P144) TFA is a peptidic transforming growth factor-beta 1 (TGF-β1) inhibitor specifically designed to block the interaction with its receptor. Disitertide (P144) TFA is also a PI3K inhibitor and an apoptosis inducer[1][2][3][4][5].

    IC50 & Target

    TGF-β1, P13K[1][2].

    In Vitro

    Disitertide (100 μg/mL) suppresses the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells[2].
    Disitertide (TGF-β1 inhibitor) abrogates the MACC1- AS1 expression in GC cells, suggesting that targeting TGFβ signaling pathway may be a potential strategy to inhibit MSC-induced stemness and chemoresistance[3].
    Disitertide (P144, 10 μg/mL to 200 μg/mL) affects proliferation, induces apoptosis as well as anoikis in A172 and U-87 MG GBM cell lines[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[2]

    Cell Line: Mouse embryo osteoblast precursor MC3T3-E1 cells.
    Concentration: 100 μg/mL.
    Incubation Time: 4 h.
    Result: Significantly suppressed the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells compared with the miR-590 group.
    In Vivo

    Disitertide (Topical application, 300 μg/mL) may promote scar maturation and clinical improvement of hypertrophic scar morphology features in an “in vivo” model in nude mice after two weeks of treatment[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Human hypertrophic scars were implanted in 60 nude mice[4].
    Dosage: 300 μg/mL was added the Lipogel.
    Administration: Topical application daily administered.
    Result: Successful shedding was achieved in 83,3% of the xenografts.
    Clinical Trial
    Molecular Weight

    1694.84

    Formula

    C₇₀H₁₁₀N₁₇O₂₄S₂F₃

    Sequence Shortening

    TSLDASIIWAMMQN

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Protect from light

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (29.50 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.5900 mL 2.9501 mL 5.9003 mL
    5 mM 0.1180 mL 0.5900 mL 1.1801 mL
    10 mM 0.0590 mL 0.2950 mL 0.5900 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (1.48 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (1.48 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    DisitertideP144P 144P-144TGF-beta/SmadPI3KApoptosisTransforming growth factor betaPhosphoinositide 3-kinaseInhibitorinhibitorinhibit

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