NFF-3 TFA
Based on 1 Customer Validation
NFF-3 TFA, the peptide, is a selective MMP substrate. NFF-3 TFA selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 TFA is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 TFA with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity.
For research use only. We do not sell to patients.
- Purity: 96.32%
- Formula: C80H111F3N22O22
- Molecular Weight:1789.87
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
IC50 & Target
[1]|
MMP-3 |
MMP-10 |
Chemical Information
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Appearance Solid
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Molecular Weight 1789.87
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Formula C80H111F3N22O22
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Color Light yellow to yellow
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Sequence
{Mca}-Arg-Pro-Lys-Pro-Val-Glu-{Nva}-Trp-Arg-Lys(DNP)-NH2
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Sequence Shortening
{Mca}-RPKPVE-{Nva}-WR-{K(DNP)}-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (55.87 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5587 mL | 2.7935 mL | 5.5870 mL | 13.9675 mL |
| 5 mM | 0.1117 mL | 0.5587 mL | 1.1174 mL | 2.7935 mL | |
| 10 mM | 0.0559 mL | 0.2793 mL | 0.5587 mL | 1.3967 mL | |
| 15 mM | 0.0372 mL | 0.1862 mL | 0.3725 mL | 0.9312 mL | |
| 20 mM | 0.0279 mL | 0.1397 mL | 0.2793 mL | 0.6984 mL | |
| 25 mM | 0.0223 mL | 0.1117 mL | 0.2235 mL | 0.5587 mL | |
| 30 mM | 0.0186 mL | 0.0931 mL | 0.1862 mL | 0.4656 mL | |
| 40 mM | 0.0140 mL | 0.0698 mL | 0.1397 mL | 0.3492 mL | |
| 50 mM | 0.0112 mL | 0.0559 mL | 0.1117 mL | 0.2793 mL |