Cat. No.: HY-N18343

Ebelin lactone

Customer Review

Based on 1 Customer Validation

Ebelin lactone is an acetylcholinesterase (AChE) inhibitor, with a K_i of 0.45 μM against human M1 muscarinic acetylcholine receptor (mAChR) and a K_i of 4.21 μM against human 5-HT2A serotonin receptor. Ebelin lactone binds to the allosteric cavity above the orthosteric site of M1 via nonpolar interactions with subtype-selective residues. Ebelin lactone binds to the cavity between transmembrane helices 4 and 5 of 5-HT2A through nonpolar interactions with orthosteric site residues. Ebelin lactone exhibits free radical scavenging activity, inhibits cancer cell proliferation, and regulates memory and cognitive processes by interacting with M1 and 5-HT2A receptors. Ebelin lactone can be used in studies related to Alzheimer's disease, breast cancer and colorectal cancer.

For research use only. We do not sell to patients.

Purity: 96.0%
CAS No.: 3649-76-1
Formula: C₃₀H₄₆O₃
Molecular Weight:454.68

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Ebelin lactone Chemical Structure

CAS No.: 3649-76-1

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]^[3]

Human 5-HT_2A Receptor

4.21 μM (Ki)

mAChR

0.45 μM (Ki)

AChE

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All mAChR Isoforms :

All 5-HT Receptor Isoforms :

In Vitro

Ebelin lactone (0.125 mg/mL; 2 hours) shows intestinal permeability in Caco-2 cells with an absorptive P_app of 121 × 10^-6 cm/s and an efflux ratio of 0.647^[1].
Ebelin lactone is non-toxic to HEK293T kidney epithelial cells (IC₅₀ ~0.9 mg/mL) and HFF-1 skin fibroblast cells (IC₅₀ ~0.862 mg/mL)^[1].
Ebelin lactone exhibits strong estimated binding affinity to 5-HT_1A, 5-HT_2A, D₁, D₂, and M₁ CNS receptors and human AChE via allosteric non-polar interactions, with the lowest binding energy (-11.71 kcal/mol) observed for the M₁ receptor^[3].
Ebelin lactone meets key CNS drug-like criteria and has very high predicted BBB penetration with moderate intestinal absorption^[3].
Ebelin lactone (up to 30 μM) binds to human M₁ (CHO cell-expressed) and 5-HT_2A (CHO cell-expressed) receptors with K_i values of 0.45 μM and 4.21 μM, respectively, and does not bind to 5-HT_1A, D₁, or D_2S receptors^[3].
Ebelin lactone (up to 30 μM; 30 min pre-incubation) does not inhibit human recombinant AChE (HEK293 cell-expressed)^[3].
Ebelin lactone inhibits proliferation of MDA-MB-468 breast cancer cells with an IC₅₀ of 0.210 mg/mL and HCT116 colorectal cancer cells with an IC₅₀ of 0.055 mg/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3649-76-1
Appearance Solid
Molecular Weight 454.68
Formula C₃₀H₄₆O₃
Color White to off-white
SMILES

C[C@]12[C@@]3(COC(C3)=O)[C@H](CC[C@]1([H])[C@@]4([C@@](C(C)([C@H](CC4)O)C)([H])CC2)C)/C=C(C)/C=C/C=C(C)/C
Structure Classification
- Steroids
Initial Source
- Plants
- Rhamnaceae
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (21.99 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1993 mL

10.9967 mL

21.9935 mL

5 mM

0.4399 mL

2.1993 mL

4.3987 mL

10 mM

0.2199 mL

1.0997 mL

2.1993 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Devaraj Reddy KN, et al. Enhanced bioavailability and efficacy of bacopa extract and ebelin lactone: preparation and biological evaluation. Asian J Biol. 2024;20(5):23-33.

[2]. Kawai K, et al. A new sapogenin in the saponins of Zizyphus jujuba, Hovenia dulcis and Bacopa monniera. Phytochemistry. 1974;13:2829-2832.

[3]. Ramasamy S, et al. In Silico and In Vitro Analysis of Bacoside A Aglycones and Its Derivatives as the Constituents Responsible for the Cognitive Effects of Bacopa monnieri. PloS one. 2015;10(5):e0126565. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1993 mL	10.9967 mL	21.9935 mL	54.9837 mL
	5 mM	0.4399 mL	2.1993 mL	4.3987 mL	10.9967 mL
	10 mM	0.2199 mL	1.0997 mL	2.1993 mL	5.4984 mL
	15 mM	0.1466 mL	0.7331 mL	1.4662 mL	3.6656 mL
	20 mM	0.1100 mL	0.5498 mL	1.0997 mL	2.7492 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Ebelin lactone

Bioactive Compound Library Max

Bioactive Compound Library

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Medicine Food Homology Compound Library

Neurotransmitter Receptor Compound Library

Anti-Alzheimer's Disease Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Protein-protein Interaction Inhibitor Library

Plant-Sourced Natural Product Library

5-HT Receptor Compound Library

Coagulation and Anti-coagulation Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

All Cholinesterase (ChE) Isoforms

All mAChR Isoforms

All 5-HT Receptor Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Ebelin lactone

Bioactive Compound Library Max

Bioactive Compound Library

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Medicine Food Homology Compound Library

Neurotransmitter Receptor Compound Library

Anti-Alzheimer's Disease Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Protein-protein Interaction Inhibitor Library

Plant-Sourced Natural Product Library

5-HT Receptor Compound Library

Coagulation and Anti-coagulation Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

All Cholinesterase (ChE) Isoforms

All mAChR Isoforms

All 5-HT Receptor Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Ebelin lactone Related Classifications

HY-N18343 Related Classifications