Tobramycin
Based on 19 publication(s) in Google Scholar
Tobramycin (Nebramycin Factor 6) is a parenterally administered, broad spectrum aminoglycoside antibiotic that is widely used in the treatment of moderate to severe bacterial infections due to sensitive organisms.Tobramycin can be used to pneumonia research caused by Pseudomonas aeruginosa.
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- Pureza : 99.93%
- No. CAS: 32986-56-4
- Fòrmula: C18H37N5O9
- Peso molecular:467.51
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) Tobramycin
More- Signal Transduct Target Ther. 2025 Dec 15;10(1):406. [Abstract]
- Nat Commun. 2022 Mar 2;13(1):1116. [Abstract]
- MedComm (2020). 2025 Jan 8;6(1):e70046. [Abstract]
- J Exp Med. 2026 Mar 2;223(3):e20241287. [Abstract]
- NPJ Biofilms Microbiomes. 2025 Mar 7;11(1):40. [Abstract]
- Food Chem. 2023 Mar 1:403:134399. [Abstract]
- Virulence. 2026 Dec 31;17(1):2646808. [Abstract]
- Int J Mol Sci. 2025 Feb 6;26(3):1381. [Abstract]
- Appl Microbiol Biotechnol. 2022 Apr;106(7):2689-2702. [Abstract]
- ACS Infect Dis. 2024 Apr 12;10(4):1327-1338. [Abstract]
- AMB Express. 2024 Dec 24;14(1):141. [Abstract]
- Infect Drug Resist. 2026 May 8;19.
- Theriogenology. 2025 Jun:239:117374. [Abstract]
- Curr Microbiol. 2021 Dec 14;79(1):12. [Abstract]
- Biofouling. 2026 Aug;42(7):771-781.
- J Microbiol Methods. 2026 Feb 25:107441. [Abstract]
- Basic Clin Androl. 2025 Mar 7;35(1):9. [Abstract]
- bioRxiv. 2024 May 10.
- bioRxiv. 2024 Feb 9.
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Microbiological Assay
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Actividad biológica
Descripciòn
IC50 & Target
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Aminoglycoside |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Vero | IC50 |
>200 μg/mL
Compound: Tobramycin
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Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
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[PMID: 28291943] |
In Vitro
Tobramycin (0-50 ng/mL; 24 hours) combinates with mycobacterium fortuitum enzyme (PodA) can greatly decreases P. aeruginosa cell viability[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Pseudomonas aeruginosa
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Concentration:2,10,50 ng/mL
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Incubation Time:24 h
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Result:Greatly decreased cell viability compared to no protein or inactive mycobacterium fortuitum enzyme (PodA) controls while PodA10 alone did not increase cell death.
In Vivo
Tobramycin (s.c., single dose) LD50 values in mice and rats are 441 and 969 mg/kg, respectively[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Murine Model of Pseudomonas aeruginosa Pneumonia Female, Swiss-Webster mice [3]
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Dosage:50, 100, 150, 214, and 400 mg/kg/day
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Administration:Intraperitoneal injection (i.p.) ,once every 4h
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Result:Had near-maximal killing of the wild-type bacteria occurred at approximately 150 mg/kg/day when tobramycin alone.
Combinated with Meropenem (HY-13678) produced near-maximal effect (i.e., bacterial cell kill) at low doses of both drugs (60 and 50 mg/kg/day for Meropenem (HY-13678) and tobramycin, respectively).
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Animal Model:Mice, rats, cats and dogs for toxicologic evaluation[4]
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Dosage:7.5,15,30,120,441,969 mg/kg
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Administration:Subcutaneous injection (s.c.), Intravenous injection (i.v.) ,Intramuscular injection(i.m.)
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Result:The s.c. LD50 values in mice and rats were 441 and 969 mg/kg, respectively.
Within 1 hour after treated, death with central nervous system depression as a precursor occurred in rats and mice.
A 100 mg/kg iv dose in chloraloseanesthetized catsproduced a moderate, transient decreasein blood pressure and a significant decrease in inspiratory volume and soleus twitch force.
Changed renal tissue in rats which were given daily sc doses of 15-120 mg/kg for 3 months.
im dose of 7.5 mg/kg for a l-month had no apparent effect on dogs, but a 30 mg/kg dose for a l-month produced severerenal damage.
Chemical Information
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No. CAS 32986-56-4
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Appearance Solid
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Peso molecular 467.51
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Fòrmula C18H37N5O9
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Color White to off-white
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SMILES
O[C@@H]([C@@H]1O[C@@]([C@@H](C[C@@H]2O)N)([H])O[C@@H]2CN)[C@H]([C@@H](C[C@@H]1N)N)O[C@@]([C@@H]([C@@H](N)[C@@H]3O)O)([H])O[C@@H]3CO
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Synonyms
Nebramycin Factor 6; Deoxykanamycin B
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Structure Classification
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Initial Source
Streptomyces tenebrarius
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (19)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Nat Commun
Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China. [Abstract]2022 Mar 2;13(1):1116. PMID: 35236849 -
MedComm (2020)
Novel antibiotics against Staphylococcus aureus without detectable resistance by targeting proton motive force and FtsH. [Abstract]2025 Jan 8;6(1):e70046. PMID: 39781293
Tobramycin purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Jan 8;6(1):e70046. [Abstract]
Time-killing curves of drug combinations between STK848198 (0.5 µg/mL) and antibiotics with FICI≤ 0.5 [SM (8 µg/mL)/ Tobramycin (32 µg/mL)/ AMP (2 µg/mL)/ fosfomycin (0.5 µg/mL)/ SIS (32 µg/mL)/ gentamycin (8 µg/mL)].
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J Exp Med
2026 Mar 2;223(3):e20241287. PMID: 41400657 -
NPJ Biofilms Microbiomes
Echinacoside reduces intracellular c-di-GMP levels and potentiates tobramycin activity against Pseudomonas aeruginosa biofilm aggregates. [Abstract]2025 Mar 7;11(1):40. PMID: 40055321 -
Food Chem
Electrochemical sensor for detecting streptomycin in milk based on label-free aptamer chain and magnetic adsorption. [Abstract]2023 Mar 1:403:134399. PMID: 36182859 -
Virulence
Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]2026 Dec 31;17(1):2646808. PMID: 41838520 -
Int J Mol Sci
Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation. [Abstract]2025 Feb 6;26(3):1381. PMID: 39941149 -
Appl Microbiol Biotechnol
2022 Apr;106(7):2689-2702. PMID: 35338386 -
ACS Infect Dis
Lusutrombopag as a Repurposing Drug in Combination with Aminoglycosides against Vancomycin-Resistant Enterococcus. [Abstract]2024 Apr 12;10(4):1327-1338. PMID: 38567846
Tobramycin purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2024 Apr 12;10(4):1327-1338. [Abstract]
Synergistic antimicrobial efficacy between LP and Tobramycin (TOB) in a murine peritonitis model. The peritonitis mice (n = 6 mice/group) were applied with 5% Kolliphor + 5% ethanol (vehicle), 30 mg/kg VAN, 30 mg/kg LP, and 30 mg/kg TOB alone or in combination twice daily for 1 day. Representative viable cell colony count was collected from the kidney and spleen.
Tobramycin purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2024 Apr 12;10(4):1327-1338. [Abstract]
Synergistic antimicrobial efficacy between LP and Tobramycin (TOB) in a murine peritonitis model. The peritonitis mice (n = 6 mice/group) were applied with 5% Kolliphor + 5% ethanol (vehicle), 30 mg/kg VAN, 30 mg/kg LP, and 30 mg/kg TOB alone or in combination twice daily for 1 day. The viable bacterial counts in the spleen were quantified.
Tobramycin purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2024 Apr 12;10(4):1327-1338. [Abstract]
Synergistic antimicrobial efficacy between LP and Tobramycin (TOB) in a murine peritonitis model. The peritonitis mice (n = 6 mice/group) were applied with 5% Kolliphor + 5% ethanol (vehicle), 30 mg/kg VAN, 30 mg/kg LP, and 30 mg/kg TOB alone or in combination twice daily for 1 day. The viable bacterial counts in kidneys were quantified.
Tobramycin purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2024 Apr 12;10(4):1327-1338. [Abstract]
Synergistic antimicrobial efficacy between LP and Tobramycin (TOB) in a murine peritonitis model. The peritonitis mice (n = 6 mice/group) were applied with 5% Kolliphor + 5% ethanol (vehicle), 30 mg/kg VAN, 30 mg/kg LP, and 30 mg/kg TOB alone or in combination twice daily for 1 day. Quantification of the inflammatory cytokine IL-6 in the serum.
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AMB Express
Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms. [Abstract]2024 Dec 24;14(1):141. PMID: 39718732
Tobramycin purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]
Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. Compound combinational activity between PVB and aminoglycosides, respectively. AMK, amikacin. GEN, gentamycin, KANA, kanamycin. TOB, Tobramycin (MICalone = 64 μg/mL; MICcombined = 256 μg/mL). SPC, spectinomycin.
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Theriogenology
Dual roles of the TLR2/TLR4/NLRP3-H-PGDS-PGD2 axis in regulating the inflammatory response in Escherichia coli-infected bovine bone marrow-derived macrophages and endometrial tissue. [Abstract]2025 Jun:239:117374. PMID: 40112766 -
Curr Microbiol
Repurposing Sitafloxacin, Prulifloxacin, Tosufloxacin, and Sisomicin as Antimicrobials Against Biofilm and Persister Cells of Pseudomonas aeruginosa. [Abstract]2021 Dec 14;79(1):12. PMID: 34905092 -
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J Microbiol Methods
Comparison of agar-based and checkerboard methods to assess phage-antimicrobial synergy in Pseudomonas aeruginosa. [Abstract]2026 Feb 25:107441. PMID: 41759663 -
Basic Clin Androl
2025 Mar 7;35(1):9. PMID: 40055615 -
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Solvente y solubilidad
In Vitro:
H2O : ≥ 100 mg/mL (213.90 mM)
DMSO : 2 mg/mL (4.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (213.90 mM); Clear solution; Need ultrasonic
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Tobramycin. [Content Brief]
[2]. VanDrisse CM, et.al. Computationally designed pyocyanin demethylase acts synergistically with tobramycin to kill recalcitrant Pseudomonas aeruginosa biofilms. Proc Natl Acad Sci U S A. 2021 Mar 23;118(12):e2022012118. [Content Brief]
[3]. Louie A, et.al. Impact of meropenem in combination with tobramycin in a murine model of Pseudomonas aeruginosa pneumonia. Antimicrob Agents Chemother. 2013 Jun;57(6):2788-92. [Content Brief]
[4]. Welles JS, et.al. Preclinical toxicology studies with tobramycin. Toxicol Appl Pharmacol. 1973 Jul;25(3):398-409. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.1390 mL | 10.6950 mL | 21.3899 mL | 53.4748 mL |
| H2O | 5 mM | 0.4278 mL | 2.1390 mL | 4.2780 mL | 10.6950 mL |
| 10 mM | 0.2139 mL | 1.0695 mL | 2.1390 mL | 5.3475 mL | |
| 15 mM | 0.1426 mL | 0.7130 mL | 1.4260 mL | 3.5650 mL | |
| 20 mM | 0.1069 mL | 0.5347 mL | 1.0695 mL | 2.6737 mL | |
| 25 mM | 0.0856 mL | 0.4278 mL | 0.8556 mL | 2.1390 mL | |
| 30 mM | 0.0713 mL | 0.3565 mL | 0.7130 mL | 1.7825 mL | |
| 40 mM | 0.0535 mL | 0.2674 mL | 0.5347 mL | 1.3369 mL | |
| 50 mM | 0.0428 mL | 0.2139 mL | 0.4278 mL | 1.0695 mL | |
| 60 mM | 0.0356 mL | 0.1782 mL | 0.3565 mL | 0.8912 mL | |
| 80 mM | 0.0267 mL | 0.1337 mL | 0.2674 mL | 0.6684 mL | |
| 100 mM | 0.0214 mL | 0.1069 mL | 0.2139 mL | 0.5347 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Tobramycin Related Classifications
HY-B0441 Related Classifications
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