Crustecdysone
Based on 14 publication(s) in Google Scholar
Crustecdysone (20-Hydroxyecdysone) is a naturally occurring ecdysteroid hormone isolated from Serratula coronata which controls the ecdysis (moulting) and metamorphosis of arthropods, it inhibits caspase activity and induces autophagy via the 20E nuclear receptor complex, EcR-USP. Crustecdysone exhibits regulatory or protective roles in the cardiovascular system. Crustecdysone is an active metabolite of Ecdysone (HY-N0179).
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- Pureza: 99.88%
- No. CAS: 5289-74-7
- Fòrmula: C27H44O7
- Peso molecular:480.63
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) Crustecdysone
More- J Adv Res. 2025 Jul 22:S2090-1232(25)00559-4. [Abstract]
- Sci Adv. 2026 Feb 13;12(7):eaea4413. [Abstract]
- J Genet Genomics. 2025 Oct 7:S1673-8527(25)00277-2. [Abstract]
- Int J Mol Sci. 2024 Dec 3;25(23):12976. [Abstract]
- ACS Omega. 2026 Apr 16;11(16):23996-24006. [Abstract]
- Transl Oncol. 2024 Jul:45:101958. [Abstract]
- Pestic Biochem Physiol. 2026 Jun 17;222:107215.
- Insect Biochem Mol Biol. 2026 Jan:186:104454. [Abstract]
- Pest Manag Sci. 2026 Jun 16. [Abstract]
- Comp Biochem Physiol Part D Genomics Proteomics. 2022 Jun:42:100981. [Abstract]
- J Insect Physiol. 2025 Dec:167:104911. [Abstract]
- Comp Biochem Physiol B Biochem Mol Biol. 2024 Jun-Jul:272:110967. [Abstract]
- Ethology. 2024 Apr 5.
- SSRN. 2025 Feb 25.
Customer Validation & Images
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Others
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Cell Proliferation/Viability Assay
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Flow Cytometry
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RT-PCR
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WB
Ver todos los productos específicos de isoformas Caspase
MoreVer todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>700 μM
Compound: 4
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Cytotoxicity against human HuH7 cells assessed as reduction in cell viability after 72 hrs by MTS assay
Cytotoxicity against human HuH7 cells assessed as reduction in cell viability after 72 hrs by MTS assay
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[PMID: 26988299] |
| Huh-7 | EC50 |
53.55 μM
Compound: 4
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Antiviral activity against Dengue virus 2 DN454009A infected in human HuH7 cells assessed as inhibition of viral RNA level measured 72 hrs post infection by qRT-PCR method
Antiviral activity against Dengue virus 2 DN454009A infected in human HuH7 cells assessed as inhibition of viral RNA level measured 72 hrs post infection by qRT-PCR method
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[PMID: 26988299] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male spontaneous hypertensive (SHR) rats[2]
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Dosage:5 or 10 mg/kg
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Administration:Intraperitoneal injection; 5 or 10 mg/kg , once daily, for 6 weeks
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Result:Decreased seminal vesicular weight, systolic pressure, and left ventricular dilatation caused by hypertension. Increased testicular weight and amount of β-isoform.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 5289-74-7
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Appearance Solid
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Peso molecular 480.63
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Fòrmula C27H44O7
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Color White to off-white
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SMILES
O[C@@H]1[C@H](O)C[C@](C(C=C2[C@]3([H])CC[C@@]4(C)[C@@]2(O)CC[C@]4([H])[C@](O)(C)[C@H](O)CCC(O)(C)C)=O)([H])[C@]3(C)C1
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Synonyms
20-Hydroxyecdysone
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
Publications (14)
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Journal Impact Factor
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Most Recent
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J Adv Res
SUMOylation modulates the dual functions of Krüppel homolog 1 in transcriptional regulation of Broad-Complex expression. [Abstract]2025 Jul 22:S2090-1232(25)00559-4. PMID: 40706986 -
Sci Adv
Opposing insulin signals underlie the same developmental switch across hemipteran insects. [Abstract]2026 Feb 13;12(7):eaea4413. PMID: 41686885 -
J Genet Genomics
circRNAs derived from a nuclear hormone receptor act differentially on insect metamorphosis and reproduction. [Abstract]2025 Oct 7:S1673-8527(25)00277-2. PMID: 41067527 -
Int J Mol Sci
20-Hydroxyecdysone Mediates Reproductive Diapause in Galeruca daurica via Ecdysone Receptor EcR and Nuclear Hormone Receptor HR3. [Abstract]2024 Dec 3;25(23):12976. PMID: 39684687
Crustecdysone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Dec 3;25(23):12976. [Abstract]
The effects of Crustecdysone (20E: 5 μg) treatment on diapause rate, expression level of diapause-related genes.
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ACS Omega
β‑Ecdysone-Loaded Gelatin Methacryloyl Hydrogel Accelerates Diabetic Wound Healing by Improving Angiogenesis. [Abstract]2026 Apr 16;11(16):23996-24006. PMID: 42077834 -
Transl Oncol
20-hydroxyecdysone suppresses bladder cancer progression via inhibiting USP21: A mechanism associated with deubiquitination and degradation of p65. [Abstract]2024 Jul:45:101958. PMID: 38663220
Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]
Several bladder cancer cell lines (J82, 5637, T24, UMUC3 and SW780) and normal human urothelial cell line (SV-HUC-1) were incubated with different concentrations (from 0 to 100 μM) of 20-HE for 24 h. Then, all cells were collected for cell viability calculation using CCK-8 analysis.
Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]
Bladder cancer cell lines T24 and UMUC3 were incubated with Crustecdysone (20-HE: 2.5, 5, or 10 μM). After 24 hours of treatment, apoptosis in the bladder cancer cells was analyzed by flow cytometry.
Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]
Bladder cancer cell lines T24 and UMUC3 were incubated with Crustecdysone (20-HE: 2.5, 5, or 10 μM). After 24 hours of treatment, the expression level of Bcl-2 mRNA was analyzed by RT-qPCR.
Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]
Bladder cancer cell lines T24 and UMUC3 were incubated with Crustecdysone (20-HE: 2.5, 5, or 10 μM). After 24 hours of treatment, Western blot analysis was performed to determine the expression level of cleaved Caspase-3 protein.
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Insect Biochem Mol Biol
Temperature-responsive spatial-temporal regulation underlies phenotypic plasticity of body pigmentation in Eastern honey bee. [Abstract]2026 Jan:186:104454. PMID: 41325970 -
Pest Manag Sci
Targeting the 20E-responsive miR-2788-Treh1 axis disrupts chitin biosynthesis and ovarian development in the grassland pest Galeruca daurica. [Abstract]2026 Jun 16. PMID: 42299103 -
Comp Biochem Physiol Part D Genomics Proteomics
Transcriptome-wide identification of microRNAs in response to 20-hydroxyecdysone in Galeruca daurica. [Abstract]2022 Jun:42:100981. PMID: 35276449 -
J Insect Physiol
MicroRNA miR-iab-8 modulates the diapause of Ostrinia furnacalis larvae by targeting the circadian clock gene cycle and affecting the ecdysone signaling pathway. [Abstract]2025 Dec:167:104911. PMID: 41285277 -
Comp Biochem Physiol B Biochem Mol Biol
2024 Jun-Jul:272:110967. PMID: 38521445 -
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Solvente y solubilidad
DMSO : 100 mg/mL (208.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 8.33 mg/mL (17.33 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (459 KB)
- English - EN (459 KB)
- Français - FR (459 KB)
- Deutsch - DE (459 KB)
- Norwegian - NO (459 KB)
- Español - ES (459 KB)
- Swedish - SV (459 KB)
- Italian - IT (459 KB)
- Korean - KR (459 KB)
- Portuguese - PT (459 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Liu H, et al. E93 predominantly transduces 20-hydroxyecdysone signaling to induce autophagy and caspase activity in Drosophila fat body. Insect Biochem Mol Biol. 2014 Feb;45:30-9. [Content Brief]
[2]. Phungphong S, et al. 20-Hydroxyecdysone attenuates cardiac remodeling in spontaneously hypertensive rats. Steroids. 2017 Oct;126:79-84. [Content Brief]
[3]. Minglei Lu, et al. Ecdysone Elicits Chronic Renal Impairment via Mineralocorticoid-Like Pathogenic Activities. Cell Physiol Biochem. 2018;49(4):1633-1645. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.0806 mL | 10.4030 mL | 20.8060 mL | 52.0151 mL |
| 5 mM | 0.4161 mL | 2.0806 mL | 4.1612 mL | 10.4030 mL | |
| 10 mM | 0.2081 mL | 1.0403 mL | 2.0806 mL | 5.2015 mL | |
| 15 mM | 0.1387 mL | 0.6935 mL | 1.3871 mL | 3.4677 mL | |
| DMSO | 20 mM | 0.1040 mL | 0.5202 mL | 1.0403 mL | 2.6008 mL |
| 25 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0806 mL | |
| 30 mM | 0.0694 mL | 0.3468 mL | 0.6935 mL | 1.7338 mL | |
| 40 mM | 0.0520 mL | 0.2601 mL | 0.5202 mL | 1.3004 mL | |
| 50 mM | 0.0416 mL | 0.2081 mL | 0.4161 mL | 1.0403 mL | |
| 60 mM | 0.0347 mL | 0.1734 mL | 0.3468 mL | 0.8669 mL | |
| 80 mM | 0.0260 mL | 0.1300 mL | 0.2601 mL | 0.6502 mL | |
| 100 mM | 0.0208 mL | 0.1040 mL | 0.2081 mL | 0.5202 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Crustecdysone Related Classifications
HY-N6979 Related Classifications
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