KD 5170
Based on 1 Customer Validation
KD 5170 is a pan inhibitor of histone deacetylases (HDACs) and exhibits broad spectrum antitumor activity in vitro and in vivo.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.13%
- No. CAS: 940943-37-3
- Fòrmula: C20H25N3O5S2
- Peso molecular:451.56
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | EC50 |
0.59 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human 786-0 cells
Cytotoxicity against human 786-0 cells
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[PMID: 18954983] |
| HCT-116 | EC50 |
0.14 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human HCT116 cells
Cytotoxicity against human HCT116 cells
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[PMID: 18954983] |
| HeLa | EC50 |
0.045 μM
Compound: Table 1, R10C1, KD5170
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Inhibition of HDAC in human HeLa cells assessed as histone H3 hyperacetylation
Inhibition of HDAC in human HeLa cells assessed as histone H3 hyperacetylation
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[PMID: 18954983] |
| HeLa | EC50 |
25 nM
Compound: 27, KD5170
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Inhibition of HDAC in human HeLa cells assessed as histone H3 hyperacetylation after 7 hrs
Inhibition of HDAC in human HeLa cells assessed as histone H3 hyperacetylation after 7 hrs
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10.1039/C1MD00199J |
| HeLa | EC50 |
325 nM
Compound: 27, KD5170
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Inhibition of HDAC in human HeLa cells assessed as alpha-tubulin acetylation after 7 hrs
Inhibition of HDAC in human HeLa cells assessed as alpha-tubulin acetylation after 7 hrs
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10.1039/C1MD00199J |
| HeLa | IC50 |
45 nM
Compound: 27, KD5170
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Inhibition of HDAC isolated from human HeLa cell nuclear extracts using Fluor de Lys as substrate after 30 mins by fluorescence assay
Inhibition of HDAC isolated from human HeLa cell nuclear extracts using Fluor de Lys as substrate after 30 mins by fluorescence assay
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10.1039/C1MD00199J |
| HL-60 | EC50 |
0.39 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human HL60 cells
Cytotoxicity against human HL60 cells
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[PMID: 18954983] |
| HT-29 | EC50 |
1 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human HT-29 cells
Cytotoxicity against human HT-29 cells
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[PMID: 18954983] |
| MDA-MB-435 | EC50 |
0.18 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human MDA-MB-435 cells
Cytotoxicity against human MDA-MB-435 cells
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[PMID: 18954983] |
| NCI-H460 | EC50 |
0.23 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human H460 cells
Cytotoxicity against human H460 cells
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[PMID: 18954983] |
| PC-3 | EC50 |
0.74 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human PC3 cells
Cytotoxicity against human PC3 cells
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[PMID: 18954983] |
| SK-OV-3 | EC50 |
0.76 μM
Compound: Table 1, R10C1, KD5170
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Cytotoxicity against human SKOV3 cells
Cytotoxicity against human SKOV3 cells
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[PMID: 18954983] |
Chemical Information
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No. CAS 940943-37-3
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Appearance Solid
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Peso molecular 451.56
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Fòrmula C20H25N3O5S2
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Color White to yellow
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SMILES
O=S(NC1=CC=C(C(CSC(C)=O)=O)C=N1)(C2=CC=C(OCCCN(C)C)C=C2)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (221.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.54 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.54 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (268 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2145 mL | 11.0727 mL | 22.1455 mL | 55.3636 mL |
| 5 mM | 0.4429 mL | 2.2145 mL | 4.4291 mL | 11.0727 mL | |
| 10 mM | 0.2215 mL | 1.1073 mL | 2.2145 mL | 5.5364 mL | |
| 15 mM | 0.1476 mL | 0.7382 mL | 1.4764 mL | 3.6909 mL | |
| 20 mM | 0.1107 mL | 0.5536 mL | 1.1073 mL | 2.7682 mL | |
| 25 mM | 0.0886 mL | 0.4429 mL | 0.8858 mL | 2.2145 mL | |
| 30 mM | 0.0738 mL | 0.3691 mL | 0.7382 mL | 1.8455 mL | |
| 40 mM | 0.0554 mL | 0.2768 mL | 0.5536 mL | 1.3841 mL | |
| 50 mM | 0.0443 mL | 0.2215 mL | 0.4429 mL | 1.1073 mL | |
| 60 mM | 0.0369 mL | 0.1845 mL | 0.3691 mL | 0.9227 mL | |
| 80 mM | 0.0277 mL | 0.1384 mL | 0.2768 mL | 0.6920 mL | |
| 100 mM | 0.0221 mL | 0.1107 mL | 0.2215 mL | 0.5536 mL |
KD 5170 Related Classifications
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