Obtusin
Based on 1 Customer Validation
Obtusin is an orally active human monoamine oxidase A (hMAO-A) inhibitor discovered from the seeds of Cassia obtusifolia, with a Ki value of 6.15 μM. Obtusin inhibits high glucose-induced activation of the MAPKs/NF-κB/VEGF pathway and downregulates the expression of Poldip2, Nox4, VCAM-1, and HIF-1α, thereby alleviating oxidative stress, endothelial activation, and angiogenesis-related functions. Obtusin ameliorates diabetic retinopathy in vivo. Obtusin is used in research related to diabetes and neurodegenerative diseases (anxiety and depression).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.10%
- No. CAS: 70588-05-5
- Fòrmula: C18H16O7
- Peso molecular:344.32
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
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hMAO-A 11.12 μM (IC50) |
hMAO-A 6.15 μM (Ki) |
HIF-1α |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HRMECs
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Concentration:10 and 20 μM
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Incubation Time:48 h
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Result:Determined 10 μM and 20 μM obtusin for 48 h as optimal conditions.
Dose-dependently suppressed proliferation and migration.
Inhibited tube-forming ability dose-dependently.
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Cell Line:HRMECs
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Concentration:10 and 20 μM
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Incubation Time:2 h
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Result:Significantly inhibited VEGFA mRNA expression.
Markedly reduced intracellular ROS levels.
Effectively reversed changes in Poldip2, Nox4, VCAM-1, and HIF-1α protein expression.
Reversed changes in MAPKs/NF-κB/VEGF pathway proteins (p-p38, p-p65, VEGFA).
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BLKS/J Lepr db mutant mice (seven-week-old, male)[1]
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Dosage:5 and 10 mg/kg/day
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Administration:p.o.; daily; 12 weeks
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Result:Reduced blood glucose levels from weeks 12 to 20 and improved body weight by week 14.
Corrected abnormal lipid profiles by reducing serum TC, TG, and LDL levels at 20 weeks.
Reduced GCL edema, increased cell numbers in the INL and ONL, and improved retinal thickness and morphology.
Reduced vascular leaks, improved retinal vascular permeability, and diminished overall fluorescence intensity.
Significantly reduced pericyte loss and the formation of acellular capillaries.
Reduced ROS levels across all retinal layers.
Alleviated structural abnormalities in retinal ganglion cells and vascular endothelial cells in a dose-dependent manner.
Reduced glial cell activation (IBA1 and GFAP) and preserved ganglion cell numbers (BRN3A) in a dose-dependent manner.
Significantly reduced the elevated levels of iNOS, IL-1β, IL-6, and VEGFA in the retinas.
- RNA extraction experimental
- Human pluripotent stem cell endothelial-cell differentiation
- Vascular/Branching Fractal Analysis
- ROS/oxidative-stress fluorescent staining
- Endothelial Tube Formation Assay
- Research Protocol for Inflammation-related Diseases
- Research Protocol for Metabolic Diseases
- Endothelial Cell Migration/Angiogenic Sprouting Assay
Chemical Information
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No. CAS 70588-05-5
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Appearance Solid
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Peso molecular 344.32
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Fòrmula C18H16O7
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Color Yellow to orange
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SMILES
O=C1C2=C(C=C(C)C(O)=C2OC)C(C3=CC(OC)=C(OC)C(O)=C13)=O
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Structure Classification
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
In Vitro:
DMSO : 5 mg/mL (14.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (291 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9043 mL | 14.5214 mL | 29.0428 mL | 72.6069 mL |
| 5 mM | 0.5809 mL | 2.9043 mL | 5.8086 mL | 14.5214 mL | |
| 10 mM | 0.2904 mL | 1.4521 mL | 2.9043 mL | 7.2607 mL |
Obtusin Related Classifications
HY-N6057 Related Classifications
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