Referencia número: HY-N0047

Polyphyllin I

5 Cited Publications

Revisión del cliente

Based on 5 publication(s) in Google Scholar

Polyphyllin I is a bioactive constituent extracted from Paris polyphylla, has strong anti-tumor activity. Polyphyllin I is an activator of the JNK signaling pathway and is an inhibitor of PDK1/Akt/mTOR signaling. Polyphyllin I induces autophagy, G2/M phase arrest and apoptosis.

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Pureza: 99.91%
No. CAS: 50773-41-6
Fòrmula: C₄₄H₇₀O₁₆
Peso molecular:855.02

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Polyphyllin I Chemical Structure

No. CAS: 50773-41-6

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

JNK signaling^[2]
PDK1/Akt/mTOR signaling^[3]

Ver todos los productos específicos de isoformas JNK :

Ver todos los productos específicos de isoformas mTOR :

Ver todos los productos específicos de isoformas Akt :

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	4.74 μM Compound: PPI	Cytotoxicity against human Bel7402 cells after 24 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 24 hrs by MTT assay	[PMID: 27623551]
HepG2	IC₅₀	4.01 μM Compound: PPI	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 27623551]
NCI-H1299	IC₅₀	<1 μM Compound: PSI	Cytotoxicity against human NCI-H1299 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H1299 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]
NCI-H446	IC₅₀	<1 μM Compound: PSI	Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]
NCI-H460	IC₅₀	<1 μM Compound: PSI	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]
NCI-H520	IC₅₀	<1 μM Compound: PSI	Cytotoxicity against human NCI-H520 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H520 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]

In Vitro

Polyphyllin I (0.625-10 mg/mL; 24-72 h) inhibits the proliferation of three non-small cell lung cancer (NSCLC) cell lines, with the IC₅₀s of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively^[1].
Polyphyllin I (2.5 mg/mL; 6-24 h) induces apoptosis of A549 cells^[1].
Polyphyllin I (0.25-2 μM; 24 h) increase in LC3-II expression and decrease in P62 expression in HGC-27 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

A549, H460, and SK-MES-1 cells^[1]
Concentration:

24, 48, 72 hours
Incubation Time:

24, 48, 72 hours
Result:

Markedly reduced proliferation capacity in vitro and in a concentration-dependent manner.

In Vivo

Polyphyllin I (1.5 mg/kg; i.p. twice daily from day 2 to day 11) significantly inhibit the tumor growth of A549 cells in the nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude mice (5 weeks old) were s.c. inoculated with A549 cells^[1]
Dosage:

1.5 mg/kg
Administration:

I.p. twice daily from day 2 to day 11
Result:

The tumor growth rate was much lower than that in cisplatin group and PBS group.

Chemical Information

No. CAS 50773-41-6
Appearance Solid
Peso molecular 855.02
Fòrmula C₄₄H₇₀O₁₆
Color White to off-white
SMILES

C[C@H](CC1)CO[C@]21O[C@@]3([H])C[C@@]4([H])[C@@]5([H])[C@]([C@](CC[C@H](O[C@]6([H])O[C@H](CO)[C@@H](O[C@]7([H])O[C@@H](CO)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)C9)(C)C9=CC5)([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C
Structure Classification
- Saccharides
- Steroids
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

All WBBio/Physico-chemical AssayCell Imaging/StainingRT-PCR

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Journal Impact Factor

Int J Mol Sci

Polyphyllin I Inhibits the Metastasis of Cervical Cancer Through the Regulation of the β-Catenin Signaling Pathway. [Abstract]

2025 May 12;26(10):4630. PMID: 40429774

Polyphyllin I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 12;26(10):4630. [Abstract]

The thermostability of Polyphyllin I (PPI) on the β-catenin protein was confirmed through CETSA.

Polyphyllin I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 12;26(10):4630. [Abstract]

SPR assay for the identification of Polyphyllin I (PPI) targeting the β-catenin protein.

Polyphyllin I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 12;26(10):4630. [Abstract]

An immunofluorescence assay was conducted to detect the effect of different concentrations of Polyphyllin I (PPI, 0-1 μM) on the changes in the nucleoplasmic distribution of β-catenin protein after a 6-h treatment of HO-8910PM cells.

Polyphyllin I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 12;26(10):4630. [Abstract]

qRT-PCR analysis of β-catenin mRNA levels in Polyphyllin I (PPI, 0-1 μM)-treated cells.

Polyphyllin I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 12;26(10):4630. [Abstract]

Assessment of the impact of PPI on β-catenin protein degradation; HO-8910PM cells were exposed to Polyphyllin I (PPI) (1 μM) either alone or in combination with either MG132 (20 μM), which is a proteasome inhibitor, or BAF (200 nM), which is an autophagy inhibitor, for 6 h. Subsequently, the cells were subjected to CHX (100 µg/mL) treatment for the indicated durations.
Biochim Biophys Acta Mol Basis Dis

Polyphyllin I inhibits glioblastoma progression by initiating ferroptosis via the Sirt1/Nrf2/HO-1/GPX4 signaling cascade. [Abstract]

2025 Aug 26;1872(1):168028. PMID: 40876787
Med Oncol

Polyphyllin I inhibits ovarian cancer growth by inducing G0/G1 phase arrest and inhibiting the c-Myc signaling pathway. [Abstract]

2025 Jun 12;42(7):254. PMID: 40506557
FASEB J

Polyphyllin I attenuates cognitive impairments and reduces AD-like pathology through CIP2A-PP2A signaling pathway in 3XTg-AD mice. [Abstract]

2020 Dec;34(12):16414-16431. PMID: 33070372

Polyphyllin I purchased from MedChemExpress. Usage Cited in: FASEB J. 2020 Dec;34(12):16414-16431. [Abstract]

HEK293-T cells are co-transfected with pFUW vector and tau plasmids, or pFUW-CIP2A and tau plasmids, with or without Polyphyllin I (PPI; 0.5 or 1.0 μM) incubation for 48 h.
SSRN

2025 Jul 17.

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (116.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1696 mL

5.8478 mL

11.6956 mL

5 mM

0.2339 mL

1.1696 mL

2.3391 mL

10 mM

0.1170 mL

0.5848 mL

1.1696 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.83 mg/mL (0.97 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (281 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Kong M, et al. Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft. Acta Biochim Biophys Sin (Shanghai). 2010 Nov;42(11):827-33. [Content Brief]

[2]. Liu J, et al. Polyphyllin I induces G2/M phase arrest and apoptosis in U251 human glioma cells via mitochondrial dysfunction and the JNK signaling pathway. Acta Biochim Biophys Sin (Shanghai). 2017 Jun 1;49(6):479-486. [Content Brief]

[3]. He J, et al. Polyphyllin I induces autophagy and cell cycle arrest via inhibiting PDK1/Akt/mTOR signal and downregulating cyclin B1 in human gastric carcinoma HGC-27 cells. Biomed Pharmacother. 2019 Sep;117:109189. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1696 mL	5.8478 mL	11.6956 mL	29.2391 mL
	5 mM	0.2339 mL	1.1696 mL	2.3391 mL	5.8478 mL
	10 mM	0.1170 mL	0.5848 mL	1.1696 mL	2.9239 mL
	15 mM	0.0780 mL	0.3899 mL	0.7797 mL	1.9493 mL
	20 mM	0.0585 mL	0.2924 mL	0.5848 mL	1.4620 mL
	25 mM	0.0468 mL	0.2339 mL	0.4678 mL	1.1696 mL
	30 mM	0.0390 mL	0.1949 mL	0.3899 mL	0.9746 mL
	40 mM	0.0292 mL	0.1462 mL	0.2924 mL	0.7310 mL
	50 mM	0.0234 mL	0.1170 mL	0.2339 mL	0.5848 mL
	60 mM	0.0195 mL	0.0975 mL	0.1949 mL	0.4873 mL
	80 mM	0.0146 mL	0.0731 mL	0.1462 mL	0.3655 mL
	100 mM	0.0117 mL	0.0585 mL	0.1170 mL	0.2924 mL

Keywords

Polyphyllin I

5 Cited Publications

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Polyphyllin I

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Publications Citing Use of MedChemExpress (MCE) Polyphyllin I

Ver todos los productos específicos de isoformas JNK

Ver todos los productos específicos de isoformas mTOR

Ver todos los productos específicos de isoformas Akt

Actividad biológica

Chemical Information