HDAC-IN-27

Name: HDAC-IN-27
Brand: MedChemExpress
SKU: HY-142690
Rating: 4.5 (1 reviews)

Cat. No.: HY-142690 Purity: 98.12%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

HDAC-IN-27 (Compound 11h) is a potent, orally active class I HDAC-selective inhibitor with IC₅₀ values ranging from 0.43 to 3.01 nM against HDAC1-3. HDAC-IN-27 exhibits both in vivo and in vitro antitumor activity. HDAC-IN-27 demonstrates significant anti-proliferative activity against acute myeloid leukemia (AML) cell lines by inducing apoptosis and histone acetylation (AcHH3 and AcHH4). HDAC-IN-27 can be used for research in acute myeloid leukemia (AML).

For research use only. We do not sell to patients.

HDAC-IN-27 Chemical Structure

CAS No. : 2763368-89-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of HDAC-IN-27:

HDAC-IN-27 dihydrochloride In-stock

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View All HDAC Isoform Specific Products:

View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

HDAC1

3.01 nM (IC₅₀)

HDAC2

18.54 nM (IC₅₀)

HDAC3

0.435 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	GI₅₀	69.6 nM Compound: 9; 11h	Antiproliferative activity against p53-null human HL-60 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against p53-null human HL-60 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
HL-60	IC₅₀	26.21 nM Compound: 11h	Antiproliferative activity against human HL-60 cells assessed as reduction in cell viability after 48 hrs by multiplate reader method Antiproliferative activity against human HL-60 cells assessed as reduction in cell viability after 48 hrs by multiplate reader method	[PMID: 34942071]
Kasumi 1	GI₅₀	17.49 nM Compound: 9; 11h	Antiproliferative activity against human Kasumi 1 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against human Kasumi 1 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
MOLM-13	IC₅₀	40.4 nM Compound: 11h	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method	[PMID: 34942071]
MV4-11	GI₅₀	37.3 nM Compound: 9; 11h	Antiproliferative activity against human MV4-11 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against human MV4-11 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
MV4-11	IC₅₀	19.17 nM Compound: 11h	Antiproliferative activity against human MV4-11 AML cells assessed as cell viability measured after 48 hrs by by multiplate reader method Antiproliferative activity against human MV4-11 AML cells assessed as cell viability measured after 48 hrs by by multiplate reader method	[PMID: 34942071]
MV4-11	IC₅₀	19.2 nM Compound: 9; 11h	Antiproliferative activity against human MV4-11 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against human MV4-11 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
NB-4	GI₅₀	31.87 nM Compound: 9; 11h	Antiproliferative activity against p53-null human NB4 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against p53-null human NB4 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
RS4-11	IC₅₀	31.52 nM Compound: 11h	Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method	[PMID: 34942071]
U-937	IC₅₀	60.51 nM Compound: 11h	Antiproliferative activity against human U-937 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human U-937 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 34942071]

In Vitro

In wt-p53 MV4-11 cells, HDAC-IN-27 (11h) leads to pro-caspase-3 cleavage, apparent apoptotic cell death, and accumulation of sub-G1 cell population while inducing G2/M arrest in HL60 cells with no evidence of apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

350.41

Formula

C₂₀H₂₂N₄O₂

CAS No.

2763368-89-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC2=CC=CC=C2N1)NCC3=CC=C(C=C3)C(NNCCC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (47.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8538 mL	14.2690 mL	28.5380 mL
5 mM	0.5708 mL	2.8538 mL	5.7076 mL
10 mM	0.2854 mL	1.4269 mL	2.8538 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.12%

Select Batch:

Data Sheet (269 KB) SDS (251 KB)

COA (245 KB) HNMR (265 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Jiang Y, et al. Potent Hydrazide-Based HDAC Inhibitors with a Superior Pharmacokinetic Profile for Efficient Treatment of Acute Myeloid Leukemia In Vivo. J Med Chem. 2022;65(1):285-302. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8538 mL	14.2690 mL	28.5380 mL	71.3450 mL
	5 mM	0.5708 mL	2.8538 mL	5.7076 mL	14.2690 mL
	10 mM	0.2854 mL	1.4269 mL	2.8538 mL	7.1345 mL
	15 mM	0.1903 mL	0.9513 mL	1.9025 mL	4.7563 mL
	20 mM	0.1427 mL	0.7134 mL	1.4269 mL	3.5672 mL
	25 mM	0.1142 mL	0.5708 mL	1.1415 mL	2.8538 mL
	30 mM	0.0951 mL	0.4756 mL	0.9513 mL	2.3782 mL
	40 mM	0.0713 mL	0.3567 mL	0.7134 mL	1.7836 mL

HDAC-IN-27 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HDAC-IN-272763368-89-2HDACApoptosisHistone deacetylasespro-caspase-3 cleavagecell deathHL60 cellsG2/M arrestacute myeloid leukemiaMV4-11apoptosisInhibitorinhibitorinhibit

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HDAC-IN-27

Customer Review

Other Forms of HDAC-IN-27:

HDAC-IN-27 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HDAC Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

HDAC-IN-27 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

HDAC-IN-27 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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