Azaserine
Based on 5 publication(s) in Google Scholar
Azazerine (CI-337) is a competitive inhibitor of glutamine amidotransferase. Azaserine is an antibiotic, it shows antibacterial activities. Azazerine shows anti-tumor activities and it may also act as a tumor inducer. Azazerine can be used for the research of cancer and infection.
For research use only. We do not sell to patients.
- Purity: 99.57%
- CAS No.: 115-02-6
- Formula: C5H7N3O4
- Molecular Weight:173.13
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Azaserine
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Cell Imaging/Staining
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All Antibiotic Isoforms
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Biological Activity
Azaserine (100 μg/mL; 6 h) causes some specific types of morphological changes of the sensitive organisms such as the characteristic elongation of Candida albicans cells[2].
Azaserine (0-10 μM) inhibits the growth of E. coli strains with MIC values of 12.11, 51.9, 69.2 and 69.2 μg/mL for UTH 4, UTH 7036, UTH 7048 and UTH 7049[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats[1]
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Dosage:5 mg/kg
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Administration:Intraperitoneal injection; 5 mg/kg once or twice a week for 6 month
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Result:After 1 year most pancreases from treated rats were diffusely abnormal and contained many hyperplastic nodules and adenomas and more than rats one-quarter showed pancreatic adenocarcinoma.
Chemical Information
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CAS No. 115-02-6
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Appearance Solid
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Molecular Weight 173.13
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Formula C5H7N3O4
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Color White to off-white
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SMILES
N[C@@H](COC(C=[N+]=[N-])=O)C(O)=O
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Synonyms
CI-337; O-Diazoacetyl-L-serine; P-165
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Rep
2024 Jan 30;43(2):113724. PMID: 38294905 -
Acta Biochim Biophys Sin (Shanghai)
2023 Mar 20;55(8):1288-1300. PMID: 36942991
Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]
Fluorescence images of BODIPY-C11-stained SW1990 pancreatic cancer cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or medium containing 2 mM glutamine supplemented with 50 μM diazooxonorleucine (Don) or 10 μM Azaserine (Aza). Cells treated with 10 μM RSL3 or 20 μM erastin were used as positive controls. Blue, DAPI; green, BODIPY-C11 (oxidized).
Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]
Fluorescence images of BODIPY-C11-stained SU.8686 pancreatic cancer cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or medium containing 2 mM glutamine supplemented with 50 μM diazooxonorleucine (Don) or 10 μM Azaserine (Aza). Cells treated with 10 μM RSL3 or 20 μM erastin were used as positive controls. Blue, DAPI; green, BODIPY-C11 (oxidized).
Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]
Proportion of BODIPY-C11 evaluated by flow cytometry in SU.8686 cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or media containing 2 mM glutamine supplemented with 50 μM Don or 10 μM Azaserine.
Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]
Proportion of BODIPY-C11 evaluated by flow cytometry in SW1990 cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or media containing 2 mM glutamine supplemented with 50 μM Don or 10 μM Azaserine.
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Vet Med Sci
Glucose Transport by Follicle-Stimulating Hormone Is Mediated Through the Akt/FOXO1 Pathway in Ovine Granulosa Cells. [Abstract]2025 Mar;11(2):e70294. PMID: 40065595
Azaserine purchased from MedChemExpress. Usage Cited in: Vet Med Sci. 2025 Mar;11(2):e70294. [Abstract]
To study the effect of glucose on the development of GCs as mediated by GLUT, the glucose inhibitor Azaserine (5 µM) was added to GC cultures, which eliminated the FSH-induced glucose uptake capacity of the GCs.
Azaserine purchased from MedChemExpress. Usage Cited in: Vet Med Sci. 2025 Mar;11(2):e70294. [Abstract]
Azaserine (5 μM) significantly reduced the viability of the GCs.
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Res Sq
De novo purine synthesis reprograms the macrophage inflammatory response and the immune response in sepsis. [Abstract]2026 Feb 19:rs.3.rs-8887742. PMID: 41756441 -
Solvent & Solubility
H2O : 50 mg/mL (288.80 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
[1]. Longnecker DS, Curphey TJ. Adenocarcinoma of the pancreas in azaserine-treated rats. Cancer Res. 1975 Aug;35(8):2249-58. [Content Brief]
[3]. Williams MV, Tritz GJ. Studies on the modes of action of azaserine inhibition of Escherichia coli. Potentiation of phenylalanine reversal. J Antimicrob Chemother. 1977 Jan;3(1):65-77. [Content Brief]
[4]. Catane R, et al. Azaserine, DON, and azotomycin: three diazo analogs of L-glutamine with clinical antitumor activity. Cancer Treat Rep. 1979 Jun;63(6):1033-8. [Content Brief]
[5]. Lyons SD, et al. Cytotoxic mechanisms of glutamine antagonists in mouse L1210 leukemia. J Biol Chem. 1990 Jul 5;265(19):11377-81. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.7760 mL | 28.8800 mL | 57.7601 mL | 144.4002 mL |
| 5 mM | 1.1552 mL | 5.7760 mL | 11.5520 mL | 28.8800 mL | |
| 10 mM | 0.5776 mL | 2.8880 mL | 5.7760 mL | 14.4400 mL | |
| 15 mM | 0.3851 mL | 1.9253 mL | 3.8507 mL | 9.6267 mL | |
| 20 mM | 0.2888 mL | 1.4440 mL | 2.8880 mL | 7.2200 mL | |
| 25 mM | 0.2310 mL | 1.1552 mL | 2.3104 mL | 5.7760 mL | |
| 30 mM | 0.1925 mL | 0.9627 mL | 1.9253 mL | 4.8133 mL | |
| 40 mM | 0.1444 mL | 0.7220 mL | 1.4440 mL | 3.6100 mL | |
| 50 mM | 0.1155 mL | 0.5776 mL | 1.1552 mL | 2.8880 mL | |
| 60 mM | 0.0963 mL | 0.4813 mL | 0.9627 mL | 2.4067 mL | |
| 80 mM | 0.0722 mL | 0.3610 mL | 0.7220 mL | 1.8050 mL | |
| 100 mM | 0.0578 mL | 0.2888 mL | 0.5776 mL | 1.4440 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Azaserine Related Classifications
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