IRF5-CPP5 

IRF5-CPP5 is a cytosolic peptide that selectively targeting human IRF5 wirh a K_d of 0.53 μM. IRF5-CPP5 disrupts IRF5 homodimerization and inhibits its nuclear translocation without altering IRF5 phosphorylation levels. IRF5-CPP5 inhibits proinflammatory cytokine (IL-6, IL-1β, TNF-α) production. IRF5-CPP5 can be used for the research of systemic lupus erythematosus^[1].

IRF5-CPP5 binds to recombinant monomeric IRF5 (222 to 425) with a submicromolar K_d of 0.53 μM^[1].
IRF5-CPP5 inhibits homodimerization of recombinant full-length IRF5 S430D and WT variants with IC₅₀ values of 15.3 and 15.4 μM^[1].
IRF5-CPP5 (1-10 μM; 1 h) dose-dependently inhibits R848 (HY-13740 -induced endogenous IRF5 homodimerization in THP-1 human monocytic cells^[1].
IRF5-CPP5 (5 μM; 30-60 min) penetrates and colocalizes with endogenous IRF5 in human primary CD19⁺ B cells, CD14⁺ monocytes, and BDCA2⁺ CD123⁺ pDCs^[1].
IRF5-CPP5 (0.62-50 μM; 30 min) concentration-dependently inhibits R848-induced IL12p40 production in human primary PBMCs^[1].
IRF5-CPP5 (0.62-16.07 μM) concentration-dependently inhibits R848-induced IRF5 nuclear translocation in human primary CD14⁺ monocytes and CD19⁺ B cells^[1].
IRF5-CPP5 (3.5-15 μM; 1 h) significantly inhibits LPS(HY-D1056) - and R848-induced proinflammatory cytokine (IL-6, IL-1β, TNF-α) production and transcript expression in human primary MDMs^[1].
IRF5-CPP5 (0.62-50 μM) concentration-dependently inhibits CpGA-induced IFNα production in human primary BDCA2⁺ CD123⁺ pDCs^[1].
IRF5-CPP5 (0.62-16.07 μM; 30 min) concentration-dependently inhibits SLE serum-induced IRF5 and phosphorylated IRF5 (pSer462) nuclear translocation in human primary CD14⁺ monocytes, CD19⁺ B cells, and BDCA2⁺ CD123⁺ pDCs, without altering total pIRF5 levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3294.95

C₁₆₀H₂₄₅N₃₇O₃₆S

1601299-92-6

Solid

White to off-white

Met-Ala-Asn-Leu-Gly-Tyr-Trp-Leu-Leu-Ala-Leu-Phe-Val-Thr-Tyr-Trp-Thr-Asp-Leu-Gly-Leu-Val-Lys-Lys-Arg-Pro-Lys-Pro

MANLGYWLLALFVTYWTDLGLVKKRPKP

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Banga J, et al. Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization[J]. Science advances, 2020, 6(20): eaay1057.  [Content Brief]