Cat. No.: HY-N0433

Astragaloside II

(Synonyms: Astrasieversianin VIII)

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Astragaloside II is an orally active Cycloartane-type triterpene glycoside. Astragaloside II can be extracted from Astragalus membranaceus. Astragaloside II inhibits Autophagy, decreases pro-inflammatory cytokines (IL-6, IL-1β), HIF-α, p-p65, p-IκB and increases SOD. Astragaloside II regulates immunity and reduces inflammatory responses. Astragaloside II can be used in the research of diseases such as liver cancer, osteoporosis, immunosuppressive diseases, and ulcerative colitis.

For research use only. We do not sell to patients.

Purity: 99.45%
CAS No.: 84676-89-1
Formula: C₄₃H₇₀O₁₅
Molecular Weight:827.01

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Astragaloside II Chemical Structure

CAS No.: 84676-89-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Interleukin Related Isoforms :

All HIF/HIF Prolyl-Hydroxylase Isoforms :

All NF-κB Isoforms :

In Vitro

Astragaloside II (0.1 nM-10 μM; 48-72 h) significantly promotes the cell viability of rat primary osteoblasts in a concentration-dependent manner^[1].
Astragaloside II (10-30 nM; 48-96 h) significantly enhances the proliferation of primary splenocytes induced by ConA, alloantigen or anti-CD3^[3].
Astragaloside II (80 μM; 48 h) sensitizes Bel-7402/FU cells to 5-fluorouracil (HY-90006)-induced cell death via suppression of autophagy^[4].
Astragaloside II (1 μM; 48 h) inhibits the levels of HIF-α, p-p65 and p-IκB in Lipopolysaccharide-stimulated CCD-18Co cells, exerting an anti-inflammatory effect^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

Bel-7402/FU
Concentration:

40 μM, 80 μM, 160 μM, 320 μM, 80 μM
Incubation Time:

48 h
Result:

Decreased the expression of autophagy-related proteins LC3-II and Beclin-1.

Cell Viability Assay^[5]

Cell Line:

CCD-18Co
Concentration:

0.1 μM, 0.33 μM, 1 μM, 3 μM
Incubation Time:

48 h
Result:

Significantly decreased the viability of CCD-18Co cells at 3 μM.
Had very limited cytotoxicity at 1 μM.

In Vivo

Astragaloside II (30-50 mg/kg; p.o.; once per day; 10 days) alleviates the symptoms of DSS (HY-116282)-induced ulcerative colitis in mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male BABL/c mice (20-22 g) with DSS-induced ulcerative colitis^[5]
Dosage:

30 mg/kg (dissolved in 2% Tween 20 in PBS), 50 mg/kg (dissolved in 2% Tween 20 in PBS)
Administration:

Oral gavage, once per day, for 10 days
Result:

Significantly reduced the DAI score.
Markedly prevented the body weight loss, increased the colon length, decreased the levels of pro-inflammatory cytokines IL-6, TNF-α, IL-1β, NO, MPO and MDA, and increased the level of SOD in colon tissues.
Decreased the expression of inflammatory proteins HIF-α, p-p65 and p-IκB.

Chemical Information

CAS No. 84676-89-1
Appearance Solid
Molecular Weight 827.01
Formula C₄₃H₇₀O₁₅
Color White to off-white
SMILES

O[C@H]1[C@H](O)[C@@H](OC(C)=O)[C@]([H])(O[C@@H]2C(C)(C)[C@@]([C@@H](O[C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)C[C@]4([H])[C@@]56CC[C@@]7(C)[C@@]4(C)C[C@H](O)[C@@]7([C@]8(C)O[C@H](C(O)(C)C)CC8)[H])([H])[C@]5(C6)CC2)OC1
Synonyms

Astrasieversianin VIII
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

All Cell Proliferation/Viability Assay

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Journal Impact Factor

J Mol Histol

Astragaloside II suppresses colorectal cancer progression by upregulating LGALS4 expression. [Abstract]

2026 Jun 29;57(4):199. PMID: 42319491
Am J Transl Res

Astragaloside II alleviates the symptoms of experimental ulcerative colitis in vitro and in vivo. [Abstract]

2019 Nov 15;11(11):7074-7083. PMID: 31814910

Astragaloside II purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2019 Nov 15;11(11):7074-7083. [Abstract]

AS II (Astragaloside II) attenuates oxidative stress damage in LPS-stimulated CCD-18Co cells via decreasing the production of inflammatory factors. CCD-18Co cells are treated with LPS (1 μg/mL) for 0, 12, 24 and 48 h. In addition, CCD-18Co cells are treated with 1 μM AS II and 1 μg/mL LPS for 48 h. The level of IL-6 in the culture media is measured with ELISA. The level of TNF-α in the culture media is measured with ELISA. The level of IL-β in the culture media is measured with ELISA. CCD-18Co c

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (120.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2092 mL

6.0459 mL

12.0918 mL

5 mM

0.2418 mL

1.2092 mL

2.4184 mL

10 mM

0.1209 mL

0.6046 mL

1.2092 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kong XH, et al. Astragaloside II induces osteogenic activities of osteoblasts through the bone morphogenetic protein-2/MAPK and Smad1/5/8 pathways. Int J Mol Med. 2012 Jun;29(6):1090-8. [Content Brief]

[2]. Huang C, et al. Reversal of P-glycoprotein-mediated multidrug resistance of human hepatic cancer cells by Astragaloside II. J Pharm Pharmacol. 2012 Dec;64(12):1741-50. [Content Brief]

[3]. Chun-ping Wan, et al. Astragaloside II triggers T cell activation through regulation of CD45 protein tyrosine phosphatase activity. Acta Pharmacol Sin. 2013 Apr;34(4):522-30. [Content Brief]

[4]. Wang M, et al. Astragaloside II sensitizes human hepatocellular carcinoma cells to 5-fluorouracil via suppression of autophagy. J Pharm Pharmacol. 2017 Jun;69(6):743-752. [Content Brief]

[5]. Qiao C, et al. Astragaloside II alleviates the symptoms of experimental ulcerative colitis in vitro and in vivo. Am J Transl Res. 2019 Nov 15;11(11):7074-7083. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2092 mL	6.0459 mL	12.0918 mL	30.2294 mL
	5 mM	0.2418 mL	1.2092 mL	2.4184 mL	6.0459 mL
	10 mM	0.1209 mL	0.6046 mL	1.2092 mL	3.0229 mL
	15 mM	0.0806 mL	0.4031 mL	0.8061 mL	2.0153 mL
	20 mM	0.0605 mL	0.3023 mL	0.6046 mL	1.5115 mL
	25 mM	0.0484 mL	0.2418 mL	0.4837 mL	1.2092 mL
	30 mM	0.0403 mL	0.2015 mL	0.4031 mL	1.0076 mL
	40 mM	0.0302 mL	0.1511 mL	0.3023 mL	0.7557 mL
	50 mM	0.0242 mL	0.1209 mL	0.2418 mL	0.6046 mL
	60 mM	0.0202 mL	0.1008 mL	0.2015 mL	0.5038 mL
	80 mM	0.0151 mL	0.0756 mL	0.1511 mL	0.3779 mL
	100 mM	0.0121 mL	0.0605 mL	0.1209 mL	0.3023 mL

Keywords

Astragaloside II

2 Cited Publications

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Astragaloside II

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Publications Citing Use of MedChemExpress (MCE) Astragaloside II

All Interleukin Related Isoforms

All HIF/HIF Prolyl-Hydroxylase Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Astragaloside II Related Classifications

HY-N0433 Related Classifications