Cat. No.: HY-N7085

Citropten

(Synonyms: 5,7-Dimethoxycoumarin; Limettin)

Customer Review

Based on 1 Customer Validation

Citropten (5, 7-dimethoxycoumarin) is one of the coumarin derivatives. Citropten is an effective oral anticancer agent. Citropten has anti-proliferative activity against A2058 and B16 melanoma cells. Citropten exerts anti-inflammatory effects through the NFκB and MAPK signaling pathways. Citropten acts as an antidepressant through heat shock protein-70, monoamine oxidase-A and inhibition of apoptosis .

For research use only. We do not sell to patients.

Purity: 99.69%
CAS No.: 487-06-9
Formula: C₁₁H₁₀O₄
Molecular Weight:206.20

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Citropten Chemical Structure

CAS No.: 487-06-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All JNK Isoforms :

All p38 MAPK Isoforms :

All ERK Isoforms :

In Vitro

Citropten (10-500 mM; 72 h) inhibits the proliferation in A2058 human melanoma cell line^[1].
Citropten (0-40 μM; 24 h) reduces NFκB and MAPK signaling pathway in activated Jurkat and HT-29 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

A2058 human melanoma cell
Concentration:

10, 50, 100, 150 and 500 mM
Incubation Time:

72 h
Result:

Inhibited cell proliferation in a concentration‐dependent manner.

Cell Proliferation Assay^[2]

Cell Line:

Activated Jurkat and HT-29 cells
Concentration:

0, 10, 20 and 40 μM
Incubation Time:

24 h
Result:

Inhibited the phosphorylation levels of IκBα, ERK, p38 and JNK.

In Vivo

Citropten (10 mg/kg; Oral administration; Every day; 7 days) has anti-inflammatory effects and modulates T cell and intestinal epithelial cell activity in mouse models of colitis induced by DSS (HY-116282)^[2].
Citropten (10 mg/kg; Intraperitoneal injection; Single injection) exerts an antidepressant effect by increasing the level of heat shock protein-70 and inhibiting the level of monoamine oxidase-A in rat models of chronic mild stress depression^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

DSS (HY-116282) treated C57BL/6J female mice aged 6-8 weeks old^[2]
Dosage:

10 mg/kg
Administration:

Oral administration (p.o.); every day; 7 days
Result:

Ameliorated the severity of inflammation on colonic tissues.
Reduced cytokine (TNFα, IL-1β and IL-8) production on colonic tissues.
Attenuated the activity of effector T cells.

Animal Model:

Chronic mild stress treated male adult Sprague-Dawley rats (around 190 g)^[3]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p.), Single injection
Result:

Prevented the chronic mild stress induced damage to locomotor activity.
Inhibited chronic mild stress induced increase in level of monoamine oxidase-A.
Inhibited the chronic mild stress induced increase in level ofcaspase-3.
Increased the level of heat shock protein-70 in the rat hippocampus.

Chemical Information

CAS No. 487-06-9
Appearance Solid
Molecular Weight 206.20
Formula C₁₁H₁₀O₄
Color White to light yellow
SMILES

O=C1OC2=C(C(OC)=CC(OC)=C2)C=C1
Synonyms

5,7-Dimethoxycoumarin; Limettin
Structure Classification
- Phenylpropanoids
- Coumarins
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (121.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8497 mL

24.2483 mL

48.4966 mL

5 mM

0.9699 mL

4.8497 mL

9.6993 mL

10 mM

0.4850 mL

2.4248 mL

4.8497 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (10.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (10.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wolny D, et al. Antiproliferative effect of valproic acid and 5,7-dimethoxycoumarin against A2058 human melanoma cells. Acta Pol Pharm. 2014 Nov-Dec;71(6):1056-9. [Content Brief]

[2]. Lee HS, et al. Ameliorative Effect of Citropten Isolated from Citrusaurantifolia Peel Extract as a Modulator of T Cell and Intestinal Epithelial Cell Activity in DSS-Induced Colitis. Molecules. 2022 Jul 20;27(14):4633. [Content Brief]

[3]. Yang W, et al. 5,7-Dimethoxycoumarin prevents chronic mild stress induced depression in rats through increase in the expression of heat shock protein-70 and inhibition of monoamine oxidase-A levels. Saudi J Biol Sci. 2018 Feb;25(2):253-258. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.8497 mL	24.2483 mL	48.4966 mL	121.2415 mL
	5 mM	0.9699 mL	4.8497 mL	9.6993 mL	24.2483 mL
	10 mM	0.4850 mL	2.4248 mL	4.8497 mL	12.1242 mL
	15 mM	0.3233 mL	1.6166 mL	3.2331 mL	8.0828 mL
	20 mM	0.2425 mL	1.2124 mL	2.4248 mL	6.0621 mL
	25 mM	0.1940 mL	0.9699 mL	1.9399 mL	4.8497 mL
	30 mM	0.1617 mL	0.8083 mL	1.6166 mL	4.0414 mL
	40 mM	0.1212 mL	0.6062 mL	1.2124 mL	3.0310 mL
	50 mM	0.0970 mL	0.4850 mL	0.9699 mL	2.4248 mL
	60 mM	0.0808 mL	0.4041 mL	0.8083 mL	2.0207 mL
	80 mM	0.0606 mL	0.3031 mL	0.6062 mL	1.5155 mL
	100 mM	0.0485 mL	0.2425 mL	0.4850 mL	1.2124 mL

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Citropten

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All JNK Isoforms

All p38 MAPK Isoforms

All ERK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Citropten Related Classifications

HY-N7085 Related Classifications