MY-1B
Based on 1 publication(s) in Google Scholar
MY-1B is a covalent inhibitor of the RNA Methyltransferase NSUN2 (IC50: 1.3 μM). MY-1B stereoselectively ligands active-site cysteine residues (C271) of NSUN2. MY-1B can stereoselectively and covalently bind to PSME1, disrupting the proteasome regulatory complex and downregulating the presentation of specific MHC-I subtypes.
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- Purity: 99.94%
- CAS No.: 2929308-79-0
- 화학식: C22H18BrN3O2
- 분자량:436.30
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보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MY-1B
More
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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RT-PCR
All DNA Methyltransferase Isoforms
More
Biological Activity
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DCAF1 |
MY-1B (5 or 20 µM, 3 h) stereoselectively and site-specifically engage C271 of NSUN2 in the human prostate cancer cell line[3].
MY-1B (2.5-20 µM, 0-2000 s) inhibits NSUN2 methyltransferase activity with Kinact/Ki values of 67±15 M[1].
MY-1B (6.25-100 μM, 2 h) serves as a stereo- and site-selective covalent ligand for C1113 of DCAF1 in HEK293T cells[2].
Fluresence Assay:[2]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2929308-79-0
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Appearance Solid
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분자량 436.30
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화학식 C22H18BrN3O2
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Color White to off-white
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SMILES
O=C([C@H]1[C@@H](C2=CC=C(C=C2)Br)CN1C(C=C)=O)NC3=CC=CC4=C3N=CC=C4
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Cancer Immunol Immunother
NSUN2/ALYREF axis-driven m5C methylation enhances PD-L1 expression and facilitates immune evasion in non-small-cell lung cancer. [Abstract]2025 Mar 3;74(4):132. PMID: 40029463
MY-1B purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Mar 3;74(4):132. [Abstract]
MY-1B exhibited dose-dependent cytotoxicity in tumor cells, whereas no significant toxicity was observed in normal lung epithelial cells BEAS-2B.
MY-1B purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Mar 3;74(4):132. [Abstract]
The specificity of NSUN2 inhibitor was assessed by WB assay. The results demonstrated that MY-1B treatment significantly reduced NSUN2 protein levels in H1299 and A549 cells.
MY-1B purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Mar 3;74(4):132. [Abstract]
The specificity of NSUN2 inhibitor was assessed by RT-qPCR assay. The results demonstrated that MY-1B treatment significantly reduced NSUN2 RNA levels in H1299 and A549 cells.
용액&용해도
DMSO : ≥ 100 mg/mL (229.20 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Tao Y, et al. Chemical Proteomic Discovery of Isotype-Selective Covalent Inhibitors of the RNA Methyltransferase NSUN2. Angew Chem Int Ed Engl. 2023 Dec 18;62(51):e202311924. [Content Brief]
[2]. Tao Y, et al. Targeted Protein Degradation by Electrophilic PROTACs that Stereoselectively and Site-Specifically Engage DCAF1. J Am Chem Soc. 2022 Oct 12;144(40):18688-18699. [Content Brief]
[3]. Ng BW, et al. Discovery of chemical probes that perturb protein complexes using size exclusion chromatography and chemical proteomics. Mol Cell. 2023 May 18;83(10):1544-1546. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2920 mL | 11.4600 mL | 22.9200 mL | 57.3000 mL |
| 5 mM | 0.4584 mL | 2.2920 mL | 4.5840 mL | 11.4600 mL | |
| 10 mM | 0.2292 mL | 1.1460 mL | 2.2920 mL | 5.7300 mL | |
| 15 mM | 0.1528 mL | 0.7640 mL | 1.5280 mL | 3.8200 mL | |
| 20 mM | 0.1146 mL | 0.5730 mL | 1.1460 mL | 2.8650 mL | |
| 25 mM | 0.0917 mL | 0.4584 mL | 0.9168 mL | 2.2920 mL | |
| 30 mM | 0.0764 mL | 0.3820 mL | 0.7640 mL | 1.9100 mL | |
| 40 mM | 0.0573 mL | 0.2865 mL | 0.5730 mL | 1.4325 mL | |
| 50 mM | 0.0458 mL | 0.2292 mL | 0.4584 mL | 1.1460 mL | |
| 60 mM | 0.0382 mL | 0.1910 mL | 0.3820 mL | 0.9550 mL | |
| 80 mM | 0.0287 mL | 0.1433 mL | 0.2865 mL | 0.7163 mL | |
| 100 mM | 0.0229 mL | 0.1146 mL | 0.2292 mL | 0.5730 mL |
MY-1B Related Classifications
HY-158301 Related Classifications
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