m-PEG2000-NHS ester  (Synonyms: mPEG2000-SC; mPEG2000-Succinimidyl ester)

m-PEG2000-NHS ester (mPEG2000-SC) is a reagent with both cell adhesion inhibition and peptide conjugation functions. The NHS ester group of m-PEG2000-NHS ester forms stable amide bonds with primary amine-containing molecules (e.g., the N-terminus of MMP-2-cleavable octapeptide) to generate mPEG-peptide intermediates for liposome surface modification. When m-PEG2000-NHS ester is immobilized on a cystamine-modified gold surface, it can construct an in vitro model for cell adhesion kinetic studies, and higher PEG density and thicker layers correlate with lower cell adhesion rates. m-PEG2000-NHS ester can synthesize MMP-2-responsive PEGylated lipid conjugates to achieve MMP-triggered dePEGylation in the tumor microenvironment. m-PEG2000-NHS ester can be used in studies related to colon cancer and other conditions^[1][2].

m-PEG2000-NHS ester (10 μL/h; 8 h) creates a PEG thickness gradient (0 to 16 nm) that reduces the adhesion rate of BAVEC-1 cells, with near-zero adhesion rate at the 16 nm thick gradient center when delivered into a 3% agarose gel overlay on cystamine-coated gold substrates^[1].
m-PEG2000-NHS ester (1:5 molar ratio; overnight, 2 h, 48 h) successfully forms an amide bond with the N-terminus of an MMP-2-cleavable octapeptide, which is then conjugated to DOPE to create an MMP-2-responsive PEG-lipid conjugate^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2000 (Average)

(C₂H₄O)nC₇H₉NO₅

92451-01-9

Solid

White to off-white

COCCOCC(ON1C(CCC1=O)=O)=O.[n]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Mougin K, et al. Construction of a tethered poly(ethylene glycol) surface gradient for studies of cell adhesion kinetics. Langmuir. 2005;21(11):4809-4812.  [Content Brief]

  [2]. Askarizadeh A, et al. Surface-modified cationic liposomes with a matrix metalloproteinase-degradable polyethylene glycol derivative improved doxorubicin delivery in murine colon cancer. J Liposome Res. 2024;34(2):221-238.  [Content Brief]