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Cat. No.: HY-13703

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Nimustine

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Nimustine is an alkylating agent, which induces DNA double-strand breaks (DSBs) and inter-strand crosslinks (ICLs), thereby activating the DNA damage response (DDR) signaling pathway. Nimustine activates p38 MAPK/JNK signaling pathway, and exhibits antitumor activity.

For research use only. We do not sell to patients.

CAS No.: 42471-28-3
Formula: C₉H₁₃ClN₆O₂
Molecular Weight:272.69

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

Nimustine Chemical Structure

CAS No.: 42471-28-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All DNA/RNA Synthesis Isoforms :

All p38 MAPK Isoforms :

All JNK Isoforms :

All AP-1 Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
SF-126	IC₅₀	310 μM Compound: ACNU	Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay in presence of O6-BG Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay in presence of O6-BG	[PMID: 27041398]
SF-126	IC₅₀	320 μM Compound: ACNU	Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay	[PMID: 27041398]
SF-126	IC₅₀	360 μM Compound: ACNU	Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability pretreated with O6-BG for 2 hrs followed by addition of compound measured after 24 hrs by CCK8 assay Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability pretreated with O6-BG for 2 hrs followed by addition of compound measured after 24 hrs by CCK8 assay	[PMID: 28197307]
SF-126	IC₅₀	450 μM Compound: ACNU	Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay	[PMID: 28197307]
U-87MG ATCC	IC₅₀	0.84 μM Compound: Nimustine	Cytotoxicity against human U87MG cells after 72 hrs by MTT assay Cytotoxicity against human U87MG cells after 72 hrs by MTT assay	[PMID: 20184290]

In Vitro

Nimustine (50 μM, 72 h) activates caspase and AP-1, upregulates the expression of bim, promotes the phosphorylation of c-Jun, and induces apoptosis in cell LN-229^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

LN-229 cell line
Concentration:

50 μM
Incubation Time:

72-120 hours
Result:

Time-dependently induced apoptosis.

Western Blot Analysis^[1]

Cell Line:

LN-229
Concentration:

50 μM
Incubation Time:

24-120 hours
Result:

Increased the expressions of cleaved of bim and caspase-3/8/9.

In Vivo

Nimustine (15-30 mg/kg, iv, every two weeks for 2 doses, or once weekly for 4 doses) inhibits the tumor growth when combined with radiotherapy, exhibits toxicity in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female C3H/HeN mice with solid FM3A tumors^[2]
Dosage:

15 and 30 mg/kg
Administration:

iv, every two weeks for 2 doses (30 mg/kg), or once weekly for 4 doses (15 mg/kg)
Result:

Inhibited tumor growth. Caused weight loss and white blood cell (WBC) depression.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 42471-28-3
Molecular Weight 272.69
Formula C₉H₁₃ClN₆O₂
SMILES

O=C(NCC1=CN=C(C)N=C1N)N(CCCl)N=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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ACS Appl Mater Interfaces

Multi-Inlet Spheroid Generator for High-Throughput Combinatorial Drug Screening Based on the Tumor Microenvironment. [Abstract]

2023 Jul 12;15(27):32087-32098. PMID: 37234040

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tomicic MT, et al. Apoptosis induced by temozolomide and nimustine in glioblastoma cells is supported by JNK/c-Jun-mediated induction of the BH3-only protein BIM. Oncotarget. 2015 Oct 20;6(32):33755-68. [Content Brief]

[2]. Shimizu F, et al. Effects of combined treatment with nimustine hydrochloride and radiation on solid FM3A tumor in mice. Jpn J Cancer Res. 1987 Jul;78(7):756-62. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Amount: USD 0.00

Biological Activity
- Description
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (1)
Purity & Documentation
References

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Nimustine

Publications Citing Use of MedChemExpress (MCE) Nimustine

All DNA/RNA Synthesis Isoforms

All p38 MAPK Isoforms

All JNK Isoforms

All AP-1 Isoforms