DW14800
Based on 1 Customer Validation
DW14800 is a protein arginine methyltransferase 5 (PRMT5) inhibitor, with an IC50 of 17 nM. DW14800 reduces H4R3me2s levels and enhances the transcription of HNF4α, but does not alter PRMT5 expression. Anti-cancer activity.
For research use only. We do not sell to patients.
- Purity: 98.44%
- CAS No.: 2243709-60-4
- Formula: C31H36N4O3
- Molecular Weight:512.64
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Epigenetics Compound Library
HY-L005
-
Histone Modification Research Compound Library
HY-L024
-
Anti-Cancer Compound Library
HY-L025
-
Reprogramming Compound Library
HY-L039
-
Anti-Blood Cancer Compound Library
HY-L079
-
Nuclear Receptor Compound Library
HY-L126
-
Anti-Hematopathy Compound Library
HY-L171
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Methylation Compound Library
HY-L203
-
High-Throughput Bioactive Compound Library
HY-L205
-
Posttranslational Modification Library
HY-L226
All Histone Methyltransferase Isoforms
More
Biological Activity
|
PRMT5 |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| MV4-11 | GI50 |
0.036 μM
Compound: 39
|
Antiproliferative activity against human MV4-11 cells supplemented with fresh medium containing compound every 4 days measured on day 12 by Celltiter-glo luminescent cell viability assay
Antiproliferative activity against human MV4-11 cells supplemented with fresh medium containing compound every 4 days measured on day 12 by Celltiter-glo luminescent cell viability assay
|
[PMID: 30605830] |
| MV4-11 | GI50 |
0.07 μM
Compound: 39
|
Antiproliferative activity against human MV4-11 cells supplemented with fresh medium containing compound every 4 days measured on day 8 by Celltiter-glo luminescent cell viability assay
Antiproliferative activity against human MV4-11 cells supplemented with fresh medium containing compound every 4 days measured on day 8 by Celltiter-glo luminescent cell viability assay
|
[PMID: 30605830] |
Chemical Information
-
CAS No. 2243709-60-4
-
Appearance Solid
-
Molecular Weight 512.64
-
Formula C31H36N4O3
-
Color White to off-white
-
SMILES
CN(C)C1=CC=C(C(N2CCC(C=C(C(NCC(O)CN3CC(C=CC=C4)=C4CC3)=O)C=C5)=C5C2)=O)C=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (195.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (269 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9507 mL | 9.7534 mL | 19.5069 mL | 48.7672 mL |
| 5 mM | 0.3901 mL | 1.9507 mL | 3.9014 mL | 9.7534 mL | |
| 10 mM | 0.1951 mL | 0.9753 mL | 1.9507 mL | 4.8767 mL | |
| 15 mM | 0.1300 mL | 0.6502 mL | 1.3005 mL | 3.2511 mL | |
| 20 mM | 0.0975 mL | 0.4877 mL | 0.9753 mL | 2.4384 mL | |
| 25 mM | 0.0780 mL | 0.3901 mL | 0.7803 mL | 1.9507 mL | |
| 30 mM | 0.0650 mL | 0.3251 mL | 0.6502 mL | 1.6256 mL | |
| 40 mM | 0.0488 mL | 0.2438 mL | 0.4877 mL | 1.2192 mL | |
| 50 mM | 0.0390 mL | 0.1951 mL | 0.3901 mL | 0.9753 mL | |
| 60 mM | 0.0325 mL | 0.1626 mL | 0.3251 mL | 0.8128 mL | |
| 80 mM | 0.0244 mL | 0.1219 mL | 0.2438 mL | 0.6096 mL | |
| 100 mM | 0.0195 mL | 0.0975 mL | 0.1951 mL | 0.4877 mL |
DW14800 Related Classifications
HY-128579 Related Classifications
Powered by Bioz