ML132
Based on 1 Customer Validation
ML132 (NCGC-00183434) is a selective caspase 1 inhibitor with an IC50 value of 0.023 nM. ML132 has good stability and can be used as a caspase 1 molecular probe. ML132 is applicable to research in the fields of anticancer and anti-inflammatory studies.
For research use only. We do not sell to patients.
- Purity: 98.43%
- CAS No.: 1230628-71-3
- Formula: C22H28ClN5O5
- Molecular Weight:477.94
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Storage:
-80°C, protect from light, stored under nitrogen
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Highly Selective Inhibitors Library
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Multi-Target Compound Library
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Covalent Screening Library Plus
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Bioactive Compound Library
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High-Throughput Bioactive Compound Library
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All Caspase Isoforms
More
Biological Activity
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Caspase-1 0.023 nM (IC50) |
Caspase-4 1.27 μM (IC50) |
Caspase-5 0.85 μM (IC50) |
Caspase-8 4.18 μM (IC50) |
Caspase-9 2.85 μM (IC50) |
ML132 (100 h; pH 2-8) exhibits pH-dependent aqueous solution stability at 23°C, with complete stability retention at pH 8 over 100 h, reduced stability at neutral pH, and the lowest stability under acidic pH conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1230628-71-3
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Appearance Solid
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Molecular Weight 477.94
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Formula C22H28ClN5O5
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Color White to light yellow
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SMILES
ClC1=CC(C(N[C@@H](C(C)(C)C)C(N2CCC[C@H]2C(N[C@@H](CC(O)=O)C#N)=O)=O)=O)=CC=C1N
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Synonyms
NCGC-00183434
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Shipping
Shipping with dry ice.
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Storage
-80°C, protect from light, stored under nitrogen
Solvent & Solubility
DMSO : 100 mg/mL (209.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0923 mL | 10.4616 mL | 20.9231 mL | 52.3078 mL |
| 5 mM | 0.4185 mL | 2.0923 mL | 4.1846 mL | 10.4616 mL | |
| 10 mM | 0.2092 mL | 1.0462 mL | 2.0923 mL | 5.2308 mL | |
| 15 mM | 0.1395 mL | 0.6974 mL | 1.3949 mL | 3.4872 mL | |
| 20 mM | 0.1046 mL | 0.5231 mL | 1.0462 mL | 2.6154 mL | |
| 25 mM | 0.0837 mL | 0.4185 mL | 0.8369 mL | 2.0923 mL | |
| 30 mM | 0.0697 mL | 0.3487 mL | 0.6974 mL | 1.7436 mL | |
| 40 mM | 0.0523 mL | 0.2615 mL | 0.5231 mL | 1.3077 mL | |
| 50 mM | 0.0418 mL | 0.2092 mL | 0.4185 mL | 1.0462 mL | |
| 60 mM | 0.0349 mL | 0.1744 mL | 0.3487 mL | 0.8718 mL | |
| 80 mM | 0.0262 mL | 0.1308 mL | 0.2615 mL | 0.6538 mL | |
| 100 mM | 0.0209 mL | 0.1046 mL | 0.2092 mL | 0.5231 mL |
ML132 Related Classifications
HY-12412 Related Classifications
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