2. Cell Cycle/DNA Damage Epigenetics
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  4. PARP1-IN-8

PARP1-IN-8 (compound 11c) is a potent and BBB-penetrated PARP1 inhibitor, with an IC50 of 97 nM. PARP1-IN-8 shows significantly potent anti-proliferative activity against Human lung adenocarcinoma epithelial cell line A549.

For research use only. We do not sell to patients.

PARP1-IN-8 Chemical Structure

PARP1-IN-8 Chemical Structure

CAS No. : 836640-15-4

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5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 950 In-stock
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Based on 1 publication(s) in Google Scholar

PARP1-IN-8 Related Antibodies

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Description

PARP1-IN-8 (compound 11c) is a potent and BBB-penetrated PARP1 inhibitor, with an IC50 of 97 nM. PARP1-IN-8 shows significantly potent anti-proliferative activity against Human lung adenocarcinoma epithelial cell line A549[1].

IC50 & Target

PARP-1

97 nM (IC50)

In Vitro

PARP1-IN-8 (compound 11c) (0-10 μM, 24-48 h) shows significantly potent anti-proliferative activity against A549 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PARP1-IN-8 Related Antibodies

Cell Proliferation Assay

Cell Line: A549, HFF cells[1]
Concentration: 0, 0.1, 1, 10 μM
Incubation Time: 24, 48 h
Result: Showed significantly potent anti-proliferative activity against A549 cells, and didn’t display any significant cytotoxicity on HFF cells[1].
Molecular Weight

403.86

Formula

C23H18ClN3O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=CC(Cl)=C1)CCN(N=C(C2=CC=CC=C2)C3=C4C=CC=C3)C4=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (154.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4761 mL 12.3805 mL 24.7611 mL
5 mM 0.4952 mL 2.4761 mL 4.9522 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (5.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4761 mL 12.3805 mL 24.7611 mL 61.9026 mL
5 mM 0.4952 mL 2.4761 mL 4.9522 mL 12.3805 mL
10 mM 0.2476 mL 1.2381 mL 2.4761 mL 6.1903 mL
15 mM 0.1651 mL 0.8254 mL 1.6507 mL 4.1268 mL
20 mM 0.1238 mL 0.6190 mL 1.2381 mL 3.0951 mL
25 mM 0.0990 mL 0.4952 mL 0.9904 mL 2.4761 mL
30 mM 0.0825 mL 0.4127 mL 0.8254 mL 2.0634 mL
40 mM 0.0619 mL 0.3095 mL 0.6190 mL 1.5476 mL
50 mM 0.0495 mL 0.2476 mL 0.4952 mL 1.2381 mL
60 mM 0.0413 mL 0.2063 mL 0.4127 mL 1.0317 mL
80 mM 0.0310 mL 0.1548 mL 0.3095 mL 0.7738 mL
100 mM 0.0248 mL 0.1238 mL 0.2476 mL 0.6190 mL
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PARP1-IN-8 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PARP1-IN-8836640-15-4PARPpoly ADP ribose polymeraseHFF cellsA549 cellsphthalazinoneLung adenocarcinomaInhibitorinhibitorinhibit

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