2. MAPK/ERK Pathway PI3K/Akt/mTOR Autophagy Apoptosis
  3. JNK mTOR Akt PDK-1 Autophagy Apoptosis
  4. Polyphyllin I

Polyphyllin I is a bioactive constituent extracted from Paris polyphylla, has strong anti-tumor activity. Polyphyllin I is an activator of the JNK signaling pathway and is an inhibitor of PDK1/Akt/mTOR signaling. Polyphyllin I induces autophagy, G2/M phase arrest and apoptosis.

For research use only. We do not sell to patients.

Polyphyllin I Chemical Structure

Polyphyllin I Chemical Structure

CAS No. : 50773-41-6

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5 mg USD 66 In-stock
10 mg USD 110 In-stock
20 mg USD 198 In-stock
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Based on 2 publication(s) in Google Scholar

Polyphyllin I Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Polyphyllin I

WB

    Polyphyllin I purchased from MedChemExpress. Usage Cited in: FASEB J. 2020 Dec;34(12):16414-16431.  [Abstract]

    HEK293-T cells are co-transfected with pFUW vector and tau plasmids, or pFUW-CIP2A and tau plasmids, with or without Polyphyllin I (PPI; 0.5 or 1.0 μM) incubation for 48 h.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Polyphyllin I is a bioactive constituent extracted from Paris polyphylla, has strong anti-tumor activity. Polyphyllin I is an activator of the JNK signaling pathway and is an inhibitor of PDK1/Akt/mTOR signaling. Polyphyllin I induces autophagy, G2/M phase arrest and apoptosis[1][2][3].

    IC50 & Target

    JNK signaling[2]
    PDK1/Akt/mTOR signaling[3]
    In Vitro

    Polyphyllin I (0.625-10 mg/mL; 24-72 h) inhibits the proliferation of three non-small cell lung cancer (NSCLC) cell lines, with the IC50s of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively[1].
    Polyphyllin I (2.5 mg/mL; 6-24 h) induces apoptosis of A549 cells[1].
    Polyphyllin I (0.25-2 μM; 24 h) increase in LC3-II expression and decrease in P62 expression in HGC-27 cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyphyllin I Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: A549, H460, and SK-MES-1 cells[1]
    Concentration: 24, 48, 72 hours
    Incubation Time: 24, 48, 72 hours
    Result: Markedly reduced proliferation capacity in vitro and in a concentration-dependent manner.
    In Vivo

    Polyphyllin I (1.5 mg/kg; i.p. twice daily from day 2 to day 11) significantly inhibit the tumor growth of A549 cells in the nude mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c nude mice (5 weeks old) were s.c. inoculated with A549 cells[1]
    Dosage: 1.5 mg/kg
    Administration: I.p. twice daily from day 2 to day 11
    Result: The tumor growth rate was much lower than that in cisplatin group and PBS group.
    Molecular Weight

    855.02

    Formula

    C44H70O16
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@H](CC1)CO[C@]21O[C@@]3([H])C[C@@]4([H])[C@@]5([H])[C@]([C@](CC[C@H](O[C@]6([H])O[C@H](CO)[C@@H](O[C@]7([H])O[C@@H](CO)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)C9)(C)C9=CC5)([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (116.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1696 mL 5.8478 mL 11.6956 mL
    5 mM 0.2339 mL 1.1696 mL 2.3391 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.83 mg/mL (0.97 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.83 mg/mL (0.97 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.61%

    COA (255 KB) RP-HPLC (72 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.1696 mL 5.8478 mL 11.6956 mL 29.2391 mL
    5 mM 0.2339 mL 1.1696 mL 2.3391 mL 5.8478 mL
    10 mM 0.1170 mL 0.5848 mL 1.1696 mL 2.9239 mL
    15 mM 0.0780 mL 0.3899 mL 0.7797 mL 1.9493 mL
    20 mM 0.0585 mL 0.2924 mL 0.5848 mL 1.4620 mL
    25 mM 0.0468 mL 0.2339 mL 0.4678 mL 1.1696 mL
    30 mM 0.0390 mL 0.1949 mL 0.3899 mL 0.9746 mL
    40 mM 0.0292 mL 0.1462 mL 0.2924 mL 0.7310 mL
    50 mM 0.0234 mL 0.1170 mL 0.2339 mL 0.5848 mL
    60 mM 0.0195 mL 0.0975 mL 0.1949 mL 0.4873 mL
    80 mM 0.0146 mL 0.0731 mL 0.1462 mL 0.3655 mL
    100 mM 0.0117 mL 0.0585 mL 0.1170 mL 0.2924 mL
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    Polyphyllin I Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyphyllin I50773-41-6JNKmTORAktPDK-1AutophagyApoptosisc-Jun N-terminal kinaseMammalian target of RapamycinPKBProtein kinase B3-Phosphoinositide-dependent protein kinase 1Paris polyphyllaanti-tumorPDK1/Akt/mTOR signalingG2/M phaseInhibitorinhibitorinhibit

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