SJ995973
Based on 1 Customer Validation
SJ995973 (PROTAC) is a uniquely potent degrader of bromodomain and extra-terminal (BET) proteins.
(Pink: BET ligand (HY-78695); Blue: Cereblon ligand (HY-163169); Black: linker (HY-W004710)).
For research use only. We do not sell to patients.
- Purity: 98.88%
- CAS No.: 2882065-25-8
- Formula: C37H40ClN7O5S
- Molecular Weight:730.28
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All PROTACs Isoforms
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Biological Activity
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BET |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MV4-11 | IC50 |
1.83 nM
Compound: SJ995973; 26
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Cytotoxicity against human MV4-11 cells
Cytotoxicity against human MV4-11 cells
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[PMID: 36202064] |
| MV4-11 | IC50 |
3 pM
Compound: 4c/SJ995973
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Antiproliferative activity against human MV4-11 after 3 days by CellTiter-Glo assay
Antiproliferative activity against human MV4-11 after 3 days by CellTiter-Glo assay
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[PMID: 36274273] |
| MV4-11 | IC50 |
3 pM
Compound: SJ995973; 26
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Cytotoxicity against human MV4-11 cells measured after 96 hrs
Cytotoxicity against human MV4-11 cells measured after 96 hrs
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[PMID: 36202064] |
| MV4-11 | IC50 |
3 pM
Compound: SJ995973
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Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell growth incubated for 3 days by CellTiter-Glo assay
Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell growth incubated for 3 days by CellTiter-Glo assay
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[PMID: 37178483] |
Chemical Information
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CAS No. 2882065-25-8
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Appearance Solid
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Molecular Weight 730.28
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Formula C37H40ClN7O5S
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Color Off-white to light yellow
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SMILES
CC1=NN=C2[C@@H](N=C(C3=C(N12)SC(C)=C3C)C4=CC=C(C=C4)Cl)CC(NCCCCCNC(COC5=CC=C(C=C5)C6CCC(NC6=O)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (136.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3693 mL | 6.8467 mL | 13.6934 mL | 34.2334 mL |
| 5 mM | 0.2739 mL | 1.3693 mL | 2.7387 mL | 6.8467 mL | |
| 10 mM | 0.1369 mL | 0.6847 mL | 1.3693 mL | 3.4233 mL | |
| 15 mM | 0.0913 mL | 0.4564 mL | 0.9129 mL | 2.2822 mL | |
| 20 mM | 0.0685 mL | 0.3423 mL | 0.6847 mL | 1.7117 mL | |
| 25 mM | 0.0548 mL | 0.2739 mL | 0.5477 mL | 1.3693 mL | |
| 30 mM | 0.0456 mL | 0.2282 mL | 0.4564 mL | 1.1411 mL | |
| 40 mM | 0.0342 mL | 0.1712 mL | 0.3423 mL | 0.8558 mL | |
| 50 mM | 0.0274 mL | 0.1369 mL | 0.2739 mL | 0.6847 mL | |
| 60 mM | 0.0228 mL | 0.1141 mL | 0.2282 mL | 0.5706 mL | |
| 80 mM | 0.0171 mL | 0.0856 mL | 0.1712 mL | 0.4279 mL | |
| 100 mM | 0.0137 mL | 0.0685 mL | 0.1369 mL | 0.3423 mL |
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