2. Cell Cycle/DNA Damage NF-κB Immunology/Inflammation Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor
  3. Interleukin Related NF-κB HSP MyD88 Estrogen Receptor/ERR
  4. Smurf1-IN-A01

Smurf1-IN-A01 is a Smurf1 inhibitor. Smurf1-IN-A01 has anticancer activity and can be used for the research of osteoporosis and age-related macular degeneration.

For research use only. We do not sell to patients.

Smurf1-IN-A01 Chemical Structure

Smurf1-IN-A01 Chemical Structure

CAS No. : 1007647-73-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 102 In-stock
Solution
10 mM * 1 mL in DMSO USD 102 In-stock
Solid
1 mg USD 42 In-stock
5 mg USD 90 In-stock
10 mg USD 150 In-stock
25 mg USD 300 In-stock
50 mg USD 490 In-stock
100 mg USD 850 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Smurf1-IN-A01 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Smurf1-IN-A01

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Smurf1-IN-A01 is a Smurf1 inhibitor. Smurf1-IN-A01 has anticancer activity and can be used for the research of osteoporosis and age-related macular degeneration[1][2][3][4].

IC50 & Target

IL-1β

 

HSP90β

 

ERα

 

In Vitro

Smurf1-IN-A01 (10 μM, 30-60 min) blocked C-type lectin receptor-2d mediated degradation of MyD88 in RAW264.7 cells upon stimulation with β-glucans[1].
Smurf1-IN-A01 (10 μM, 24 h) attenuates the effect of enhancing the ALP activity and Alizarin red S staining in C3H10T1/2 cells[2].
Smurf1-IN-A01 (10 μM, 12 h) decreases ER alpha protein and ERE-luciferase activity in MCF-7 and T47D cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Smurf1-IN-A01 Related Antibodies
In Vivo

Smurf1-IN-A01 (10 μM, 2 μL, Injected into vitreous cavity by micro-syringe, single dose) alleviated retinal injury in retinal degeneration model mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: retinal degeneration model mice[4]
Dosage: 10 μM ,2μL
Administration: Injected into vitreous cavity by micro-syringe
Result: Reduced drusen-like spots.
Resulted outer nuclear layers wider and straighter.
Caused inner segment and outer segment hinge of photoreceptor cells disappeared.
Down-regulated NLRP3 and inhibited IL-1β.
Could decrease the death in NaIO3 retina.
Molecular Weight

512.93

Formula

C22H20ClF3N4O3S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC(C(F)(F)F)=C(Cl)C=C1)(N2CCN(C(C3=CC=C(N4C(C)=CC=N4)C=C3)=O)CC2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (121.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9496 mL 9.7479 mL 19.4958 mL
5 mM 0.3899 mL 1.9496 mL 3.8992 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.06 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.06 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

COA (248 KB) LCMS (270 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9496 mL 9.7479 mL 19.4958 mL 48.7396 mL
5 mM 0.3899 mL 1.9496 mL 3.8992 mL 9.7479 mL
10 mM 0.1950 mL 0.9748 mL 1.9496 mL 4.8740 mL
15 mM 0.1300 mL 0.6499 mL 1.2997 mL 3.2493 mL
20 mM 0.0975 mL 0.4874 mL 0.9748 mL 2.4370 mL
25 mM 0.0780 mL 0.3899 mL 0.7798 mL 1.9496 mL
30 mM 0.0650 mL 0.3249 mL 0.6499 mL 1.6247 mL
40 mM 0.0487 mL 0.2437 mL 0.4874 mL 1.2185 mL
50 mM 0.0390 mL 0.1950 mL 0.3899 mL 0.9748 mL
60 mM 0.0325 mL 0.1625 mL 0.3249 mL 0.8123 mL
80 mM 0.0244 mL 0.1218 mL 0.2437 mL 0.6092 mL
100 mM 0.0195 mL 0.0975 mL 0.1950 mL 0.4874 mL
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Smurf1-IN-A01 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Smurf1-IN-A011007647-73-5A01A 01A-01Interleukin RelatedNF-κBHSPMyD88Estrogen Receptor/ERRILNuclear factor-κBNuclear factor-kappaBHeat shock proteinsRAW264.7C3H10T1/2MCF-7T47Dretinal degeneration model miceosteoporosisage-related macular degenerationInhibitorinhibitorinhibit

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