Tivanisiran
Based on 1 Customer Validation
Tivanisiran (SYL1001) is a small interfering RNA (siRNA) targeting TRPV1 . Tivanisiran induces the degradation of TRPV1 mRNA, thereby inhibiting protein synthesis. Tivanisiran alleviates ocular discomfort and pain, and improves ocular hyperemia and tear quality. Tivanisiran is applicable to research related to dry eye disease.
For research use only. We do not sell to patients.
- Purity : 97.48%
- CAS No.: 1848224-71-4
- Molecular Weight:12068.30 (AS: 5294.6 + SS: 6125.7)
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Biological Activity
Description
IC50 & Target
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TRPV1 |
In Vitro
Tivanisiran selectively silences TRPV1 in cell lines expressing TRPV subtypes, with an IC50 of 2.67 nM, and reduces TRPV1 mRNA levels by 50%-60%, with this effect peaking at 24-48 hours post-transfection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:New Zealand White Rabbits[1]
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Dosage:multiple doses
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Administration:once daily; 4 consecutive days
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Result:Produced palpebral opening responses similar to the reference standard Capsazepine at all evaluated time-points post-capsaicin challenge.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1848224-71-4
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Appearance Solid
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Molecular Weight 12068.30 (AS: 5294.6 + SS: 6125.7)
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Color White to off-white
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SMILES
[Tivanisiran]
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Synonyms
SYL1001
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Sequence
RNA, (A-A-G-C-G-C-A-U-C-U-U-C-U-A-C-U-U-C-A), complex with RNA (U-G-A-A-G-U-A-G-A-A-G-A-U-G-C-G-C-U-U) (1:1)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (Need ultrasonic)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL; Clear solution; Need ultrasonic
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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RNA interference technology
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing gene transcription or activating RNA degradation. This mechanism was discovered in plants in 1998 by Andrew Fire and Craig Mello. Today, this phenomenon can be observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2242 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)