Cat. No.: HY-N6979

Crustecdysone

(Synonyms: 20-Hydroxyecdysone)

14 Cited Publications

Customer Review

Based on 14 publication(s) in Google Scholar

Crustecdysone (20-Hydroxyecdysone) is a naturally occurring ecdysteroid hormone isolated from Serratula coronata which controls the ecdysis (moulting) and metamorphosis of arthropods, it inhibits caspase activity and induces autophagy via the 20E nuclear receptor complex, EcR-USP. Crustecdysone exhibits regulatory or protective roles in the cardiovascular system. Crustecdysone is an active metabolite of Ecdysone (HY-N0179).

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 5289-74-7
Formula: C₂₇H₄₄O₇
Molecular Weight:480.63

Storage:

4°C, protect from light

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Crustecdysone Chemical Structure

CAS No.: 5289-74-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (14)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Crustecdysone (20-Hydroxyecdysone) is a naturally occurring ecdysteroid hormone isolated from Serratula coronata which controls the ecdysis (moulting) and metamorphosis of arthropods, it inhibits caspase activity and induces autophagy via the 20E nuclear receptor complex, EcR-USP^[1]. Crustecdysone exhibits regulatory or protective roles in the cardiovascular system^[2]. Crustecdysone is an active metabolite of Ecdysone (HY-N0179)^[3].

All Caspase Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Huh-7	CC₅₀	>700 μM Compound: 4	Cytotoxicity against human HuH7 cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human HuH7 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 26988299]
Huh-7	EC₅₀	53.55 μM Compound: 4	Antiviral activity against Dengue virus 2 DN454009A infected in human HuH7 cells assessed as inhibition of viral RNA level measured 72 hrs post infection by qRT-PCR method Antiviral activity against Dengue virus 2 DN454009A infected in human HuH7 cells assessed as inhibition of viral RNA level measured 72 hrs post infection by qRT-PCR method	[PMID: 26988299]

In Vitro

Crustecdysone induces cell autophagy and caspase-3 activity in the remodeling fat body of Drosophila^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Crustecdysone (5 or 10 mg/kg; i.p., once daily, for 6 weeks) reduces blood pressure and prevents dilated cardiac hypertrophy in SHR rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male spontaneous hypertensive (SHR) rats^[2]
Dosage:

5 or 10 mg/kg
Administration:

Intraperitoneal injection; 5 or 10 mg/kg , once daily, for 6 weeks
Result:

Decreased seminal vesicular weight, systolic pressure, and left ventricular dilatation caused by hypertension. Increased testicular weight and amount of β-isoform.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 5289-74-7
Appearance Solid
Molecular Weight 480.63
Formula C₂₇H₄₄O₇
Color White to off-white
SMILES

O[C@@H]1[C@H](O)C[C@](C(C=C2[C@]3([H])CC[C@@]4(C)[C@@]2(O)CC[C@]4([H])[C@](O)(C)[C@H](O)CCC(O)(C)C)=O)([H])[C@]3(C)C1
Synonyms

20-Hydroxyecdysone
Structure Classification
- Steroids
Initial Source
- Plants
- Compositae
- Plants
- other families
- Animals
- Endogenous metabolite
insects
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Publications (14)

All OthersCell Proliferation/Viability AssayFlow CytometryRT-PCRWB

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Journal Impact Factor

J Adv Res

SUMOylation modulates the dual functions of Krüppel homolog 1 in transcriptional regulation of Broad-Complex expression. [Abstract]

2025 Jul 22:S2090-1232(25)00559-4. PMID: 40706986
Sci Adv

Opposing insulin signals underlie the same developmental switch across hemipteran insects. [Abstract]

2026 Feb 13;12(7):eaea4413. PMID: 41686885
J Genet Genomics

circRNAs derived from a nuclear hormone receptor act differentially on insect metamorphosis and reproduction. [Abstract]

2025 Oct 7:S1673-8527(25)00277-2. PMID: 41067527
Int J Mol Sci

20-Hydroxyecdysone Mediates Reproductive Diapause in Galeruca daurica via Ecdysone Receptor EcR and Nuclear Hormone Receptor HR3. [Abstract]

2024 Dec 3;25(23):12976. PMID: 39684687

Crustecdysone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Dec 3;25(23):12976. [Abstract]

The effects of Crustecdysone (20E: 5 μg) treatment on diapause rate, expression level of diapause-related genes.
ACS Omega

β‑Ecdysone-Loaded Gelatin Methacryloyl Hydrogel Accelerates Diabetic Wound Healing by Improving Angiogenesis. [Abstract]

2026 Apr 16;11(16):23996-24006. PMID: 42077834
Transl Oncol

20-hydroxyecdysone suppresses bladder cancer progression via inhibiting USP21: A mechanism associated with deubiquitination and degradation of p65. [Abstract]

2024 Jul:45:101958. PMID: 38663220

Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]

Several bladder cancer cell lines (J82, 5637, T24, UMUC3 and SW780) and normal human urothelial cell line (SV-HUC-1) were incubated with different concentrations (from 0 to 100 μM) of 20-HE for 24 h. Then, all cells were collected for cell viability calculation using CCK-8 analysis.

Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]

Bladder cancer cell lines T24 and UMUC3 were incubated with Crustecdysone (20-HE: 2.5, 5, or 10 μM). After 24 hours of treatment, apoptosis in the bladder cancer cells was analyzed by flow cytometry.

Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]

Bladder cancer cell lines T24 and UMUC3 were incubated with Crustecdysone (20-HE: 2.5, 5, or 10 μM). After 24 hours of treatment, the expression level of Bcl-2 mRNA was analyzed by RT-qPCR.

Crustecdysone purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2024 Jul:45:101958. [Abstract]

Bladder cancer cell lines T24 and UMUC3 were incubated with Crustecdysone (20-HE: 2.5, 5, or 10 μM). After 24 hours of treatment, Western blot analysis was performed to determine the expression level of cleaved Caspase-3 protein.
Pestic Biochem Physiol

2026 Jun 17;222:107215.
Insect Biochem Mol Biol

Temperature-responsive spatial-temporal regulation underlies phenotypic plasticity of body pigmentation in Eastern honey bee. [Abstract]

2026 Jan:186:104454. PMID: 41325970
Pest Manag Sci

Targeting the 20E-responsive miR-2788-Treh1 axis disrupts chitin biosynthesis and ovarian development in the grassland pest Galeruca daurica. [Abstract]

2026 Jun 16. PMID: 42299103
Comp Biochem Physiol Part D Genomics Proteomics

Transcriptome-wide identification of microRNAs in response to 20-hydroxyecdysone in Galeruca daurica. [Abstract]

2022 Jun:42:100981. PMID: 35276449
J Insect Physiol

MicroRNA miR-iab-8 modulates the diapause of Ostrinia furnacalis larvae by targeting the circadian clock gene cycle and affecting the ecdysone signaling pathway. [Abstract]

2025 Dec:167:104911. PMID: 41285277
Comp Biochem Physiol B Biochem Mol Biol

MicroRNA miR-7-5p targets MARK2 to control metamorphosis in Galeruca daurica. [Abstract]

2024 Jun-Jul:272:110967. PMID: 38521445
Ethology

2024 Apr 5.
SSRN

2025 Feb 25.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (208.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 8.33 mg/mL (17.33 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0806 mL

10.4030 mL

20.8060 mL

5 mM

0.4161 mL

2.0806 mL

4.1612 mL

10 mM

0.2081 mL

1.0403 mL

2.0806 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (459 KB)
- English - EN (459 KB)
- Français - FR (459 KB)
- Deutsch - DE (459 KB)
- Norwegian - NO (459 KB)
- Español - ES (459 KB)
- Swedish - SV (459 KB)
- Italian - IT (459 KB)
- Korean - KR (459 KB)
- Portuguese - PT (459 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liu H, et al. E93 predominantly transduces 20-hydroxyecdysone signaling to induce autophagy and caspase activity in Drosophila fat body. Insect Biochem Mol Biol. 2014 Feb;45:30-9. [Content Brief]

[2]. Phungphong S, et al. 20-Hydroxyecdysone attenuates cardiac remodeling in spontaneously hypertensive rats. Steroids. 2017 Oct;126:79-84. [Content Brief]

[3]. Minglei Lu, et al. Ecdysone Elicits Chronic Renal Impairment via Mineralocorticoid-Like Pathogenic Activities. Cell Physiol Biochem. 2018;49(4):1633-1645. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.0806 mL	10.4030 mL	20.8060 mL	52.0151 mL
	5 mM	0.4161 mL	2.0806 mL	4.1612 mL	10.4030 mL
	10 mM	0.2081 mL	1.0403 mL	2.0806 mL	5.2015 mL
	15 mM	0.1387 mL	0.6935 mL	1.3871 mL	3.4677 mL
DMSO	20 mM	0.1040 mL	0.5202 mL	1.0403 mL	2.6008 mL
	25 mM	0.0832 mL	0.4161 mL	0.8322 mL	2.0806 mL
	30 mM	0.0694 mL	0.3468 mL	0.6935 mL	1.7338 mL
	40 mM	0.0520 mL	0.2601 mL	0.5202 mL	1.3004 mL
	50 mM	0.0416 mL	0.2081 mL	0.4161 mL	1.0403 mL
	60 mM	0.0347 mL	0.1734 mL	0.3468 mL	0.8669 mL
	80 mM	0.0260 mL	0.1300 mL	0.2601 mL	0.6502 mL
	100 mM	0.0208 mL	0.1040 mL	0.2081 mL	0.5202 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Crustecdysone

14 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Crustecdysone

All Caspase Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (14)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Crustecdysone Related Classifications

HY-N6979 Related Classifications