Cat. No.: HY-N5073

Vitexin-4''-O-glucoside

(Synonyms: 4''-O-Glucosylvitexin)

Customer Review

Based on 1 Customer Validation

Vitexin-4''-O-glucoside (4''-O-Glucosylvitexin) is an orally active natural flavonoid component with multiple pharmacological effects including antioxidation, anti-inflammation, cytoprotection and anti-apoptosis. Vitexin-4''-O-glucoside regulates the MAPK signaling pathway by downregulating the phosphorylation levels of JNK and p38, thereby blocking endoplasmic reticulum stress responses. Vitexin-4''-O-glucoside alleviates oxidative stress by reducing MDA content and upregulating the activities of SOD and CAT, attenuates inflammation by downregulating the expressions of inflammatory factors TNF-α, IL-1β and IL-6, and also reduces LDH release and inhibits caspase-3 activation. Vitexin-4''-O-glucoside effectively improves drug-induced acute liver injury and exerts significant protective effects against myocardial hypoxia/reoxygenation injury. Vitexin-4''-O-glucoside can be used in studies on acute liver injury, cardiovascular diseases and myocardial hypoxia-reoxygenation injury.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 178468-00-3
Formula: C₂₇H₃₀O₁₅
Molecular Weight:594.52

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Vitexin-4''-O-glucoside Chemical Structure

CAS No.: 178468-00-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- Parmacokinetics
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

JNK

p38

IL-6

IL-1β

TNF-α

Caspase 3

All JNK Isoforms :

All p38 MAPK Isoforms :

All Interleukin Related Isoforms :

All TNF Receptor Isoforms :

All Caspase Isoforms :

In Vitro

Vitexin-4''-O-glucoside (VOG) (1.25-40 μg/mL) alleviates Tunicamycin (HY-A0098) -induced endoplasmic reticulum stress and cell damage in AML-12 mouse hepatocytes, with significant protective effects observed at concentrations of 5.00 μg/mL and higher^[1].
Vitexin-4''-O-glucoside (120 μM; 24 h pretreatment) prevents hydrogen peroxide-induced apoptotic nuclear morphological changes in human adipose-derived stem cells^[2].
Vitexin-4''-O-glucoside (30-480 μM; 24-72 h) is non-cytotoxic to human adipose-derived stem cells at concentrations up to 480 μM over 72 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

human adipose-derived stem cells (hADSCs)
Concentration:

30 μM; 60 μM; 120 μM; 240 μM; 480 μM
Incubation Time:

24 h; 48 h; 72 h
Result:

Showed no cytotoxic effect on hADSCs at concentrations up to 480 μM across all incubation times.
Promoted hADSC viability.
Significantly increased hADSC viability.

Apoptosis Analysis^[2]

Cell Line:

human adipose-derived stem cells (hADSCs)
Concentration:

120 μM
Incubation Time:

24 h (pretreatment)
Result:

Reduced hydrogen peroxide-induced percentage of hADSCs in early apoptosis and late apoptosis/necrosis .

Parmacokinetics

Species	Dose	Route	T_1/2 (Distribution)	T_1/2β	AUC_0-t	AUC_0-∞	MRT_0-t	MRT_0-∞	CL	V_d	C_max
Rat^[4]	1.73 mg/kg	i.v.	0.11 h	0.87 h	15,602.08 μg·h/mL	15,626.16 μg·h/mL	1.17 h	1.19 h	0.002 L/h/kg	0.003 L/kg	17,706.47 μg/L

In Vivo

Vitexin-4''-O-glucoside (VOG) (30-60 mg/kg; p.o.; single dose; 2 hours prior to Acetaminophen (HY-66005) injection) dose-dependently alleviates acetaminophen-induced acute liver injury in mice^[1].
Vitexin-4''-O-glucoside (VGL) (1.73 mg/kg; i.v.; single dose) exhibits a three-compartment pharmacokinetic profile in healthy male Sprague-Dawley rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (8-week-old male, 20 g, acetaminophen-induced acute liver injury)^[1]
Dosage:

30 mg/kg; 60 mg/kg
Administration:

p.o.; single dose; 2 hours prior to acetaminophen injection
Result:

Lowered plasma ALT and AST levels in a dose-dependent manner, with 60 mg/kg showing stronger effects than 30 mg/kg.
Alleviated hepatocyte vacuolation and inflammatory infiltration, and achieved better morphological improvement at 60 mg/kg.
Decreased hepatic MDA content and elevated SOD and CAT activities, with more notable antioxidant effects at the higher dose.
Suppressed hepatic IL-1β, IL-6 and TNF-α expression, and 60 mg/kg produced a more potent anti-inflammatory effect.
Inhibited phosphorylation of JNK and p38 in a dose-dependent fashion.
Downregulated the expression of IRE-1α, GRP78 and XBP1s, and 60 mg/kg exerted superior regulatory effects on IRE-1α and GRP78.

Chemical Information

CAS No. 178468-00-3
Appearance Solid
Molecular Weight 594.52
Formula C₂₇H₃₀O₁₅
Color Light yellow to yellow
SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C([C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O[C@H]5[C@@H]([C@H]([C@@H]([C@@H](CO)O5)O)O)O)O)O)C(O)=CC(O)=C13
Synonyms

4''-O-Glucosylvitexin
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (168.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6820 mL

8.4101 mL

16.8203 mL

5 mM

0.3364 mL

1.6820 mL

3.3641 mL

10 mM

0.1682 mL

0.8410 mL

1.6820 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dong F, et al. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2025;54(3):307-317. [Content Brief]

[2]. Wei W, et al. Effects of vitexin-2"-O-rhamnoside and vitexin-4"-O-glucoside on growth and oxidative stress-induced cell apoptosis of human adipose-derived stem cells. J Pharm Pharmacol. 2014;66(7):988-997. [Content Brief]

[3]. Ying XX, et al. Pharmacokinetics of vitexin-4″-O-glucoside in rats after intravenous application. Eur J Drug Metab Pharmacokinet. 2012;37(2):109-115. [Content Brief]

[4]. Zhang W, et al. Simultaneous determination of vitexin-4''-O-glucoside, vitexin-2''-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC-ESI-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(21):1837-1844. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6820 mL	8.4101 mL	16.8203 mL	42.0507 mL
	5 mM	0.3364 mL	1.6820 mL	3.3641 mL	8.4101 mL
	10 mM	0.1682 mL	0.8410 mL	1.6820 mL	4.2051 mL
	15 mM	0.1121 mL	0.5607 mL	1.1214 mL	2.8034 mL
	20 mM	0.0841 mL	0.4205 mL	0.8410 mL	2.1025 mL
	25 mM	0.0673 mL	0.3364 mL	0.6728 mL	1.6820 mL
	30 mM	0.0561 mL	0.2803 mL	0.5607 mL	1.4017 mL
	40 mM	0.0421 mL	0.2103 mL	0.4205 mL	1.0513 mL
	50 mM	0.0336 mL	0.1682 mL	0.3364 mL	0.8410 mL
	60 mM	0.0280 mL	0.1402 mL	0.2803 mL	0.7008 mL
	80 mM	0.0210 mL	0.1051 mL	0.2103 mL	0.5256 mL
	100 mM	0.0168 mL	0.0841 mL	0.1682 mL	0.4205 mL

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Vitexin-4''-O-glucoside

Bioactive Compound Library Max

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All JNK Isoforms

All p38 MAPK Isoforms

All Interleukin Related Isoforms

All TNF Receptor Isoforms

All Caspase Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Vitexin-4''-O-glucoside Related Classifications

HY-N5073 Related Classifications