Z-DEVD-AFC
Based on 1 Customer Validation
Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence uponcleavage of the AFC fluorophore. Z-DEVD-AFC can be used to detect caspase-3-like enzymes activity.
For research use only. We do not sell to patients.
- Purity: 98.08%
- CAS No.: 1135416-11-3
- Formula: C36H38F3N5O14
- Molecular Weight:821.71
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
All Caspase Isoforms
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Biological Activity
500
380
Chemical Information
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CAS No. 1135416-11-3
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Appearance Solid
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Molecular Weight 821.71
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Formula C36H38F3N5O14
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Color White to off-white
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Sequence Shortening
zDEVD-AFC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL (60.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2170 mL | 6.0849 mL | 12.1697 mL | 30.4244 mL |
| 5 mM | 0.2434 mL | 1.2170 mL | 2.4339 mL | 6.0849 mL | |
| 10 mM | 0.1217 mL | 0.6085 mL | 1.2170 mL | 3.0424 mL | |
| 15 mM | 0.0811 mL | 0.4057 mL | 0.8113 mL | 2.0283 mL | |
| 20 mM | 0.0608 mL | 0.3042 mL | 0.6085 mL | 1.5212 mL | |
| 25 mM | 0.0487 mL | 0.2434 mL | 0.4868 mL | 1.2170 mL | |
| 30 mM | 0.0406 mL | 0.2028 mL | 0.4057 mL | 1.0141 mL | |
| 40 mM | 0.0304 mL | 0.1521 mL | 0.3042 mL | 0.7606 mL | |
| 50 mM | 0.0243 mL | 0.1217 mL | 0.2434 mL | 0.6085 mL | |
| 60 mM | 0.0203 mL | 0.1014 mL | 0.2028 mL | 0.5071 mL |