6-Thioinosine
Based on 1 Customer Validation
6-Thioinosine (6TI) is a purine antimetabolite and an anti-adipogenic agent. 6-Thioinosine reduces the mRNA levels of PPARγ and C/EBPα and downregulates the mRNA levels of PPARγ target genes (LPL, CD36, aP2, and LXRα). 6-Thioinosine exerts its anti-adipogenic effects by downregulating PPARγ through JNK-dependent iNOS upregulation. 6-Thioinosine can be used to study adipocyte dysfunction.
For research use only. We do not sell to patients.
- Purity: 99.31%
- CAS No.: 574-25-4
- Formula: C10H12N4O4S
- Molecular Weight:284.30
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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PPAR-γ |
iNOS |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| L1210 | IC50 |
0.01 μM
Compound: 6-MP ribonucleoside
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Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
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[PMID: 1906107] |
| L1210 | IC50 |
0.068 μM
Compound: 6-MP ribonucleoside
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Inhibitory concentration on multidrug-resistant L1210 leukemia cells.
Inhibitory concentration on multidrug-resistant L1210 leukemia cells.
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[PMID: 1906107] |
| S49 | EC50 |
0.25 μM
Compound: 6-thioinosine
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Cytotoxicity against wild type mouse S49 cells assessed as growth inhibition after 72 hrs by trypan blue exclusion assay
Cytotoxicity against wild type mouse S49 cells assessed as growth inhibition after 72 hrs by trypan blue exclusion assay
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[PMID: 221658] |
| S49 | EC50 |
400 μM
Compound: 6-thioinosine
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Cytotoxicity against HGPRTase-deficient mouse S49 cells assessed as growth inhibition after 72 hrs by trypan blue exclusion assay
Cytotoxicity against HGPRTase-deficient mouse S49 cells assessed as growth inhibition after 72 hrs by trypan blue exclusion assay
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[PMID: 221658] |
6-Thioinosine (1-50 µM, once every 3 days for 9 days) reduces lipid and triglyceride accumulation and GPDH activity, increases TNF α production in 3T3-L1 cells[1].
6-Thioinosine (1-25 µM, 2-14 h/once every 3 days for 9 days) inhibits the expression and transactivation activity of PPAR γ, induces NO production by upregulating the iNOS gene, induces iNOS expression by activating JNK in 3T3-L1 cells[1].
6-Thioinosine (1-25 µM, once every 3 days for 21 days) exerts an anti-adipogenic effect on adipose tissue-derived stem cells and presdipocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:3T3-L1 cells
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Concentration:1, 10, 25 µM
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Incubation Time:once every 3 days for 9 days
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Result:Reduced PPARγ mRNA levels.
Reduced the mRNA levels of C/EBPα, LPL, CD36, aP2 and LXRα.
Increased iNOS mRNA levels.
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Cell Line:3T3-L1 cells
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Concentration:1, 10, 25 µM
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Incubation Time:once every 3 days for 9 days
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Result:Reduced PPARγ protein expression levels.
Increased iNOS protein expression levels.
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Cell Line:3T3-L1 cells
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Concentration:1, 10, 25 µM
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Incubation Time:2 h
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Result:Induced JNK phosphorylation but not p38 MAPK or p42/44 MAPK phosphorylation.
Chemical Information
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CAS No. 574-25-4
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Appearance Solid
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Molecular Weight 284.30
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Formula C10H12N4O4S
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Color White to off-white
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SMILES
S=C1NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CO)O3
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Synonyms
6TI; 6-Mercaptopurine riboside
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 83.33 mg/mL (293.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5174 mL | 17.5871 mL | 35.1741 mL | 87.9353 mL |
| 5 mM | 0.7035 mL | 3.5174 mL | 7.0348 mL | 17.5871 mL | |
| 10 mM | 0.3517 mL | 1.7587 mL | 3.5174 mL | 8.7935 mL | |
| 15 mM | 0.2345 mL | 1.1725 mL | 2.3449 mL | 5.8624 mL | |
| 20 mM | 0.1759 mL | 0.8794 mL | 1.7587 mL | 4.3968 mL | |
| 25 mM | 0.1407 mL | 0.7035 mL | 1.4070 mL | 3.5174 mL | |
| 30 mM | 0.1172 mL | 0.5862 mL | 1.1725 mL | 2.9312 mL | |
| 40 mM | 0.0879 mL | 0.4397 mL | 0.8794 mL | 2.1984 mL | |
| 50 mM | 0.0703 mL | 0.3517 mL | 0.7035 mL | 1.7587 mL | |
| 60 mM | 0.0586 mL | 0.2931 mL | 0.5862 mL | 1.4656 mL | |
| 80 mM | 0.0440 mL | 0.2198 mL | 0.4397 mL | 1.0992 mL | |
| 100 mM | 0.0352 mL | 0.1759 mL | 0.3517 mL | 0.8794 mL |
6-Thioinosine Related Classifications
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