1. Apoptosis
  2. Caspase

Biotin-VAD-FMK 

Cat. No.: HY-100894 Purity: >98.0%
Handling Instructions

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

For research use only. We do not sell to patients.

Biotin-VAD-FMK Chemical Structure

Biotin-VAD-FMK Chemical Structure

CAS No. : 1135688-15-1

Size Price Stock Quantity
1 mg USD 840 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 3000 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

IC50 & Target[1]

Caspase

 

In Vitro

Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability.

In Vivo

To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases[2].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 27.3 mg/mL (40.58 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4863 mL 7.4315 mL 14.8630 mL
5 mM 0.2973 mL 1.4863 mL 2.9726 mL
10 mM 0.1486 mL 0.7432 mL 1.4863 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

672.81

Formula

C₃₀H₄₉FN₆O₈S

CAS No.

1135688-15-1

SMILES

COC(C[[email protected]@H](C(CF)=O)NC([[email protected]](C)NC([[email protected]](C(C)C)NC(CCCCCNC(CCCC[[email protected]@H]1SC[[email protected]](N2)[[email protected]@H]1NC2=O)=O)=O)=O)=O)=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Biotin-VAD-FMK
Cat. No.:
HY-100894
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Biotin-VAD-FMK

Cat. No.: HY-100894