2. Vitamin D Related/Nuclear Receptor Anti-infection Apoptosis
  3. Estrogen Receptor/ERR HBV Bcl-2 Family
  4. Estradiol benzoate

Estradiol benzoate  (Synonyms: β-Estradiol 3-benzoate; 17β-Estradiol 3-benzoate)

Cat. No.: HY-B1192 Purity: 99.90%
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Estradiol benzoate (β-Estradiol 3-benzoate) is a HBx protein inhibitor and inhibits androgen and hepatitis B virus (HBV) transcription, replication. Estradiol benzoate shows antifertility effects, anti- Toxoplasma gondii activity and can improve memory behavior of Ovariectomy (Ovx) female mice.

For research use only. We do not sell to patients.

CAS No. : 50-50-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Estradiol benzoate:

Estradiol benzoate Related Antibodies

Top Publications Citing Use of Products

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227.  [Abstract]

    Estradiol benzoate (0.3 μg; i.m.;every three days). H&E staining of the vehicle group revealed endometrial epithelial cells and stromal cells within the endometriotic lesions, along with glandular hyperplasia in the lamina propria.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227.  [Abstract]

    Morphology of endometriotic lesions excised from the peritoneum of mice. Estradiol benzoate (0.3 μg; i.m.;every three days) successfully induced EMS in mice.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15387-15410.  [Abstract]

    Estradiol benzoate (17β-Estradiol) (1 μM; drinking water) was used to sustain the MCF7 xenograft model in NCG mice.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 8;21(1):81.  [Abstract]

    Estradiol benzoate (3 μg) induced endometriosis-like lesions in diverse peritoneal locations of female mice.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2019 Dec 5:245:112181.  [Abstract]

    ICR female mice were treated with Estradiol benzoate (1 mg/kg; i.p.; 3 d). On the fourth day, the mice were injected with oxytocin (50 U/kg) to induce dysmenorrhea.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Estradiol benzoate (β-Estradiol 3-benzoate) is a HBx protein inhibitor and inhibits androgen and hepatitis B virus (HBV) transcription, replication. Estradiol benzoate shows antifertility effects, anti- Toxoplasma gondii activity and can improve memory behavior of Ovariectomy (Ovx) female mice[1][2][3][4][5].

    IC50 & Target

    Bax

     

    In Vitro

    Estradiol benzoate (5-75 μM, 24 h-48 h) has a downregulation effect on HBV translation, transcription, and replication in SBHX21 cells[3].
    Estradiol benzoate (0-500 μM, 72 h) has toxicity to Human foreskin fibroblasts (HFFs)[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Estradiol benzoate Related Antibodies

    Cell Cytotoxicity Assay[4]

    Cell Line: HFFs
    Concentration: 0-500 μM
    Incubation Time: 72 h
    Result: Inhibited HFFs at the 50% toxicity of 34.11 μM.
    In Vivo

    Estradiol benzoate (20-100 μg/kg, Subcutaneous injection, once a day for 4-5 weeks) improves learning and memory behavior of Ovx female mice[1].
    Estradiol benzoate (0.015-15000 μg/kg, Subcutaneous injection, single dose) advances male puberty in male Sprague-Dawley (SD) rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ovx mice[1]
    Dosage: 20, 100 or 200 μg/kg
    Administration: Subcutaneous injection (s.c.)
    Result: Significantly reversed the lowered uterine weight induced by Ovx.
    Had no obvious effects on locomotor activity in Ovx mice.
    Effectively reversed the changes of enlarging the width of synaptic cleft and reducing the thickness of PSD induced by Ovx.
    Increased the number and density of synaptic vesicles by 4-week replacement with EB (100 μg/kg).
    Molecular Weight

    376.49

    Formula

    C25H28O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@@H](O)CC[C@@]1([H])[C@]3([H])CCC4=C(C=CC(OC(C5=CC=CC=C5)=O)=C4)[C@@]3([H])CC2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (132.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 3.33 mg/mL (8.84 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6561 mL 13.2806 mL 26.5611 mL
    5 mM 0.5312 mL 2.6561 mL 5.3122 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.90%

    SDS (419 KB)

    COA (249 KB) HNMR (139 KB) RP-HPLC (255 KB) MS (67 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.6561 mL 13.2806 mL 26.5611 mL 66.4028 mL
    5 mM 0.5312 mL 2.6561 mL 5.3122 mL 13.2806 mL
    DMSO 10 mM 0.2656 mL 1.3281 mL 2.6561 mL 6.6403 mL
    15 mM 0.1771 mL 0.8854 mL 1.7707 mL 4.4269 mL
    20 mM 0.1328 mL 0.6640 mL 1.3281 mL 3.3201 mL
    25 mM 0.1062 mL 0.5312 mL 1.0624 mL 2.6561 mL
    30 mM 0.0885 mL 0.4427 mL 0.8854 mL 2.2134 mL
    40 mM 0.0664 mL 0.3320 mL 0.6640 mL 1.6601 mL
    50 mM 0.0531 mL 0.2656 mL 0.5312 mL 1.3281 mL
    60 mM 0.0443 mL 0.2213 mL 0.4427 mL 1.1067 mL
    80 mM 0.0332 mL 0.1660 mL 0.3320 mL 0.8300 mL
    100 mM 0.0266 mL 0.1328 mL 0.2656 mL 0.6640 mL
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    Estradiol benzoate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Estradiol50-50-0β-Estradiol 3-benzoate 17β-Estradiol 3-benzoateEstrogen Receptor/ERRHBVBcl-2 FamilyHepatitis B virusOvx miceHepG2Huh7SBHX21HepG2.2.15Human foreskin fibroblasts (HFFs)Inhibitorinhibitorinhibit

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