Cat. No.: HY-B1104

Indoprofen

(Synonyms: (±)-Indoprofe)

Customer Review

Based on 1 Customer Validation

Indoprofen ((±)-Indoprofe) activates AKT-AMPK signaling pathway, inhibits NF-κB/MAPK signaling pathway. Indoprofen exhibits anti-inflammatory and immunomodulatory activities. Indoprofen is orally active.

For research use only. We do not sell to patients.

Purity: 99.36%
CAS No.: 31842-01-0
Formula: C₁₇H₁₅NO₃
Molecular Weight:281.31

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Indoprofen Chemical Structure

CAS No.: 31842-01-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Akt Isoforms :

All AMPK Isoforms :

All NF-κB Isoforms :

All p38 MAPK Isoforms :

In Vitro

Indoprofen (1-200 μM, 48 h) promotes PGC-1α expression, induces the formation of Myh-positive myotubes, inhibits Dexamethasone (HY-14648)-induced myotube atrophy and restores Myh protein level^[1].
Indoprofen (1-200 μM, 48 h) enhances the oxidative metabolism capacity of muscles by regulating the expression of oxidative metabolism related proteins^[1].
Indoprofen (7.5-20 μM, 12 h) reduces rhHMGB1-induced production of inflammatory factor (such as TNF-α, IL-1β, IL-6 and PGE2), and reduces the expression of TLR2, TLR4 and RAGE on the THP-1 cell surface^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

C2C12
Concentration:

1-200 μM
Incubation Time:

48 h
Result:

Upregulated the expression of total-OXPHOS (oxidative phosphorylation complex) and myoglobin.

Real Time qPCR^[2]

Cell Line:

THP-1
Concentration:

7.5-20 μM
Incubation Time:

24 h
Result:

Reduced the expression of RAGE.

In Vivo

Indoprofen (2 mg/kg, po, once daily for 2-12 weeks) improves muscle mass and strength in mice, improves the muscle function in aged mice. Indoprofen prevents the reduction of myotube formation, and enhances fatigue resistance against Dexamethasone (HY-14648)-induced disuse muscle atrophy^[1].
Indoprofen (20-40 mg/kg, ip, single dose) reduces the inflammatory response and tissue damage caused by sepsis and improves the survival rate of septic mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse model^[1]
Dosage:

2 mg/kg
Administration:

po, once daily for 2-12 weeks
Result:

Improved muscle mass and strength in young and old mice.
Improved muscle function in aged mice, including running distance, grip strength, and isometric force.
Prevented muscle fiber thinning, improved muscle strength in dexamethasone-stimulated mice.

Animal Model:

Cecal ligation and puncture (CLP)-induced sepsis in mouse models^[2]
Dosage:

20-40 mg/kg
Administration:

ip, single dose
Result:

Alleviated pulmonary edema. Reduced pathological damage in the lung, heart, liver, kidney and colon. Improved the survival rate of septic mice

Chemical Information

CAS No. 31842-01-0
Appearance Solid
Molecular Weight 281.31
Formula C₁₇H₁₅NO₃
Color White to off-white
SMILES

O=C(O)C(C)C1=CC=C(N(CC2=C3C=CC=C2)C3=O)C=C1
Synonyms

(±)-Indoprofe
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (355.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5548 mL

17.7740 mL

35.5480 mL

5 mM

0.7110 mL

3.5548 mL

7.1096 mL

10 mM

0.3555 mL

1.7774 mL

3.5548 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.08 mg/mL (7.39 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (7.39 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 25 mg/mL (88.87 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 0.5% CMC/saline water

Solubility: 50 mg/mL (177.74 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (643 KB)
- English - EN (643 KB)
- Français - FR (643 KB)
- Deutsch - DE (643 KB)
- Norwegian - NO (643 KB)
- Español - ES (643 KB)
- Swedish - SV (643 KB)
- Italian - IT (643 KB)
- Korean - KR (643 KB)
- Portuguese - PT (643 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim H, Cho SC, Jeong HJ, et al. Indoprofen prevents muscle wasting in aged mice through activation of PDK1/AKT pathway. J Cachexia Sarcopenia Muscle. 2020;11(4):1070-1088. [Content Brief]

[2]. Bi X, et al., Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses. Toxicol Appl Pharmacol. 2021 Dec 15;433:115778. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5548 mL	17.7740 mL	35.5480 mL	88.8699 mL
	5 mM	0.7110 mL	3.5548 mL	7.1096 mL	17.7740 mL
	10 mM	0.3555 mL	1.7774 mL	3.5548 mL	8.8870 mL
	15 mM	0.2370 mL	1.1849 mL	2.3699 mL	5.9247 mL
	20 mM	0.1777 mL	0.8887 mL	1.7774 mL	4.4435 mL
	25 mM	0.1422 mL	0.7110 mL	1.4219 mL	3.5548 mL
	30 mM	0.1185 mL	0.5925 mL	1.1849 mL	2.9623 mL
	40 mM	0.0889 mL	0.4443 mL	0.8887 mL	2.2217 mL
	50 mM	0.0711 mL	0.3555 mL	0.7110 mL	1.7774 mL
	60 mM	0.0592 mL	0.2962 mL	0.5925 mL	1.4812 mL
	80 mM	0.0444 mL	0.2222 mL	0.4443 mL	1.1109 mL
	100 mM	0.0355 mL	0.1777 mL	0.3555 mL	0.8887 mL

Keywords

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Indoprofen

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Pyroptosis Compound Library

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Orally Active Compound Library

Glutamine Metabolism Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

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Anti-Blood Cancer Compound Library

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Human Metabolite Library

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Non-steroidal Anti-Inflammatory Compound Library

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MCE Bioactive Compound Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

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Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Lactylation Compound Library

All Akt Isoforms

All AMPK Isoforms

All NF-κB Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Indoprofen Related Classifications

Cat. No.: HY-B1104