Indoprofen
Based on 1 Customer Validation
Indoprofen ((±)-Indoprofe) activates AKT-AMPK signaling pathway, inhibits NF-κB/MAPK signaling pathway. Indoprofen exhibits anti-inflammatory and immunomodulatory activities. Indoprofen is orally active.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.36%
- No. CAS: 31842-01-0
- Fòrmula: C17H15NO3
- Peso molecular:281.31
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
In Vitro
Indoprofen (1-200 μM, 48 h) promotes PGC-1α expression, induces the formation of Myh-positive myotubes, inhibits Dexamethasone (HY-14648)-induced myotube atrophy and restores Myh protein level[1].
Indoprofen (1-200 μM, 48 h) enhances the oxidative metabolism capacity of muscles by regulating the expression of oxidative metabolism related proteins[1].
Indoprofen (7.5-20 μM, 12 h) reduces rhHMGB1-induced production of inflammatory factor (such as TNF-α, IL-1β, IL-6 and PGE2), and reduces the expression of TLR2, TLR4 and RAGE on the THP-1 cell surface[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C2C12
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Concentration:1-200 μM
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Incubation Time:48 h
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Result:Upregulated the expression of total-OXPHOS (oxidative phosphorylation complex) and myoglobin.
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Cell Line:THP-1
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Concentration:7.5-20 μM
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Incubation Time:24 h
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Result:Reduced the expression of RAGE.
In Vivo
Indoprofen (20-40 mg/kg, ip, single dose) reduces the inflammatory response and tissue damage caused by sepsis and improves the survival rate of septic mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse model[1]
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Dosage:2 mg/kg
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Administration:po, once daily for 2-12 weeks
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Result:Improved muscle mass and strength in young and old mice.
Improved muscle function in aged mice, including running distance, grip strength, and isometric force.
Prevented muscle fiber thinning, improved muscle strength in dexamethasone-stimulated mice.
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Animal Model:Cecal ligation and puncture (CLP)-induced sepsis in mouse models[2]
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Dosage:20-40 mg/kg
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Administration:ip, single dose
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Result:Alleviated pulmonary edema. Reduced pathological damage in the lung, heart, liver, kidney and colon. Improved the survival rate of septic mice
Chemical Information
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No. CAS 31842-01-0
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Appearance Solid
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Peso molecular 281.31
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Fòrmula C17H15NO3
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Color White to off-white
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SMILES
O=C(O)C(C)C1=CC=C(N(CC2=C3C=CC=C2)C3=O)C=C1
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Synonyms
(±)-Indoprofe
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (355.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (7.39 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (7.39 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (88.87 mM); Suspended solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (643 KB)
- English - EN (643 KB)
- Français - FR (643 KB)
- Deutsch - DE (643 KB)
- Norwegian - NO (643 KB)
- Español - ES (643 KB)
- Swedish - SV (643 KB)
- Italian - IT (643 KB)
- Korean - KR (643 KB)
- Portuguese - PT (643 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kim H, Cho SC, Jeong HJ, et al. Indoprofen prevents muscle wasting in aged mice through activation of PDK1/AKT pathway. J Cachexia Sarcopenia Muscle. 2020;11(4):1070-1088. [Content Brief]
[2]. Bi X, et al., Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses. Toxicol Appl Pharmacol. 2021 Dec 15;433:115778. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5548 mL | 17.7740 mL | 35.5480 mL | 88.8699 mL |
| 5 mM | 0.7110 mL | 3.5548 mL | 7.1096 mL | 17.7740 mL | |
| 10 mM | 0.3555 mL | 1.7774 mL | 3.5548 mL | 8.8870 mL | |
| 15 mM | 0.2370 mL | 1.1849 mL | 2.3699 mL | 5.9247 mL | |
| 20 mM | 0.1777 mL | 0.8887 mL | 1.7774 mL | 4.4435 mL | |
| 25 mM | 0.1422 mL | 0.7110 mL | 1.4219 mL | 3.5548 mL | |
| 30 mM | 0.1185 mL | 0.5925 mL | 1.1849 mL | 2.9623 mL | |
| 40 mM | 0.0889 mL | 0.4443 mL | 0.8887 mL | 2.2217 mL | |
| 50 mM | 0.0711 mL | 0.3555 mL | 0.7110 mL | 1.7774 mL | |
| 60 mM | 0.0592 mL | 0.2962 mL | 0.5925 mL | 1.4812 mL | |
| 80 mM | 0.0444 mL | 0.2222 mL | 0.4443 mL | 1.1109 mL | |
| 100 mM | 0.0355 mL | 0.1777 mL | 0.3555 mL | 0.8887 mL |
Indoprofen Related Classifications
HY-B1104 Related Classifications
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