Art. -Nr.: HY-171770

dAurAB5

Name: dAurAB5
Brand: MedChemExpress
SKU: HY-171770
Price: 570.00 USD
Availability: InStock
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dAurAB5 is a dual Aurora-A (DC₅₀ = 8.8 nM) and Aurora-B (DC₅₀ = 6.1 nM) PROTAC degrader. dAurAB5 induces degradation of Aurora-A and Aurora-B, reduces N-Myc levels, and decreases viability in IMR32 neuroblastoma cells. dAurAB5 downregulates the levels of AAK1, PTK2, GAK, and TTK. dAurAB5 can be used to study cancers such as neuroblastoma. (Pink: TTK ligand 2: HY-168542, Blue: Thalidomide-O-COOH: HY-103597, Black: 6-Aminocaproic acid: HY-B0236).
(Pink: Aurora A and Aurora B ligand (HY-168542); Blue: Cereblon ligand (HY-103597); Black: linker (HY-B0236)).

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.03%
CAS. Nr.: 3115765-45-9
Formel: C₄₂H₄₉N₁₁O₇
Molecular Weight:819.91

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

dAurAB5 Chemical Structure

CAS. Nr.: 3115765-45-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-171767

SK2188

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

dAurAB5 is a dual Aurora-A (DC₅₀ = 8.8 nM) and Aurora-B (DC₅₀ = 6.1 nM) PROTAC degrader. dAurAB5 induces degradation of Aurora-A and Aurora-B, reduces N-Myc levels, and decreases viability in IMR32 neuroblastoma cells. dAurAB5 downregulates the levels of AAK1, PTK2, GAK, and TTK. dAurAB5 can be used to study cancers such as neuroblastoma. (Pink: TTK ligand 2: HY-168542, Blue: Thalidomide-O-COOH: HY-103597, Black: 6-Aminocaproic acid: HY-B0236)^[1]. (Pink: Aurora A and Aurora B ligand (HY-168542); Blue: Cereblon ligand (HY-103597); Black: linker (HY-B0236)).

IC₅₀ & Target^[1]

Cereblon

Aurora-A

8.8 nM (DC₅₀)

Aurora-B

6.1 nM (DC₅₀)

Alle PROTACs Isoform-spezifische Produkte anzeigen :

Alle Aurora Kinase Isoform-spezifische Produkte anzeigen :

In Vitro

dAurAB5 (500 nM, 24 h) induces significant degradation of Aurora-A and Aurora-B, with degradation rates of 84% and 82%^[1].
dAurAB5 (500 nM, 24 h) reduces N-Myc levels by 45% in IMR32 cells^[1].
dAurAB5 (100 nM, 4 h) degrades 83% of Aurora-A and 95% of Aurora-B in IMR32 cells^[1].
dAurAB5 (200 nM, 6 h) decreases the abundance Aurora-A and the levels of AAK1, PTK2, GAK, TTK, but does not downregulated Aurora-B abundance in the MYCN-amplified Kelly neuroblastoma cells^[1].
dAurAB5 (0-1000 nM, 24 h) reduces cell viability in IMR32 cells, but has minimal cytotoxicity in HEK293 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

IMR32 cells
Concentration:

500 nM
Incubation Time:

24 h
Result:

Reduced N-Myc levels by 45%.

Western Blot Analysis^[1]

Cell Line:

IMR32 cells
Concentration:

100 nM
Incubation Time:

4 h
Result:

Degraded 83% of Aurora-A and 95% of Aurora-B.

Cell Viability Assay^[1]

Cell Line:

IMR32 cells
Concentration:

0, 10, 100, 1000 nM
Incubation Time:

24 h
Result:

Significant reduced cell viability, with 45% reduction in cell viability at 100 nM and 55% reduction in cell viability at 1 μM.

Chemical Information

CAS. Nr. 3115765-45-9
Appearance Solid
Molecular Weight 819.91
Formel C₄₂H₄₉N₁₁O₇
Color Yellow to orange
SMILES

O=C(NCCCCCC(N1CCN(C2=CC=C(NC3=NC(NC4CCCCC4)=C5N=CNC5=N3)C=C2)CC1)=O)COC6=CC=CC(C(N7C(CC8)C(NC8=O)=O)=O)=C6C7=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (121.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2196 mL

6.0982 mL

12.1965 mL

5 mM

0.2439 mL

1.2196 mL

2.4393 mL

10 mM

0.1220 mL

0.6098 mL

1.2196 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (6.10 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Nelson SE, et al. Development of Dual Aurora-A and Aurora-B Degrading PROTACs for MCYN-Amplified Neuroblastoma. ChemMedChem. 2025 Mar 3;20(5):e202400703. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2196 mL	6.0982 mL	12.1965 mL	30.4912 mL
	5 mM	0.2439 mL	1.2196 mL	2.4393 mL	6.0982 mL
	10 mM	0.1220 mL	0.6098 mL	1.2196 mL	3.0491 mL
	15 mM	0.0813 mL	0.4065 mL	0.8131 mL	2.0327 mL
	20 mM	0.0610 mL	0.3049 mL	0.6098 mL	1.5246 mL
	25 mM	0.0488 mL	0.2439 mL	0.4879 mL	1.2196 mL
	30 mM	0.0407 mL	0.2033 mL	0.4065 mL	1.0164 mL
	40 mM	0.0305 mL	0.1525 mL	0.3049 mL	0.7623 mL
	50 mM	0.0244 mL	0.1220 mL	0.2439 mL	0.6098 mL
	60 mM	0.0203 mL	0.1016 mL	0.2033 mL	0.5082 mL
	80 mM	0.0152 mL	0.0762 mL	0.1525 mL	0.3811 mL
	100 mM	0.0122 mL	0.0610 mL	0.1220 mL	0.3049 mL

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dAurAB5

SK2188

Alle PROTACs Isoform-spezifische Produkte anzeigen

Alle Aurora Kinase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

dAurAB5 Related Classifications

HY-171770 Related Classifications

Bioz