Cat. No.: HY-B0789

SU6656

24 Cited Publications

Customer Review

Based on 24 publication(s) in Google Scholar

SU6656 is a Src family kinases inhibitor with IC₅₀s of 280, 20, 130, 170 nM for Src, Yes, Lyn, and Fyn, respectively. SU6656 inhibits FAK phosphorylation at Y576/577, Y925, Y861 sites. SU6656 also inhibits p-AKT.

For research use only. We do not sell to patients.

Purity: 98.71%
CAS No.: 330161-87-0
Formula: C₁₉H₂₁N₃O₃S
Molecular Weight:371.45

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

SU6656 Chemical Structure

CAS No.: 330161-87-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (24)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Src Isoforms :

All Akt Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BaF3	IC₅₀	0.18 μM Compound: SU-6656	Growth inhibition of mouse BA/F3 cells assessed as incorporation of [3H]thymidine incorporation after 72 hrs by liquid scintillation counting Growth inhibition of mouse BA/F3 cells assessed as incorporation of [3H]thymidine incorporation after 72 hrs by liquid scintillation counting	[PMID: 20117004]
Sf9	IC₅₀	0.77 μM Compound: SU-6656	Inhibition of human recombinant His-tagged RET expressed in Sf9 insect cells Inhibition of human recombinant His-tagged RET expressed in Sf9 insect cells	[PMID: 20117004]

In Vitro

SU6656 decreases phosphorylation of Src family kinases (SFKs) in HNSCC cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SU6656 (2-4 mg/kg; i.p.; once) significantly decreases ischemic postconditioning (IPoCo) mediated increase in fall down time^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Swiss albino male mice^[5]
Dosage:

2, 4 mg/kg
Administration:

Intraperitoneal injection; once
Result:

Significantly decreased IPoCo mediated increase in fall down time.

Chemical Information

CAS No. 330161-87-0
Appearance Solid
Molecular Weight 371.45
Formula C₁₉H₂₁N₃O₃S
Color Yellow to orange
SMILES

O=S(C1=CC2=C(NC(/C2=C\C(N3)=CC4=C3CCCC4)=O)C=C1)(N(C)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (24)

All Cell Proliferation/Viability AssayWBPK/PD AnalysisCell Migration/Invasion Assay

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Journal Impact Factor

Cancer Res

Hormonal Regulation of Semaphorin 7a in ER+ Breast Cancer Drives Therapeutic Resistance. [Abstract]

2021 Jan 1;81(1):187-198. PMID: 33122307

SU6656 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2021 Jan 1;81(1):187-198. [Abstract]

Clonogenic growth of MCF7 EV or SEMA7A OE cells treated with 5 µM SU6656 for 72 h. Clonogenic data are normalized to vehicle controls.
Nat Commun

INSIG1/2 succination mediated by the moonlighting function of ADSL promotes lipogenesis and liver tumorigenesis. [Abstract]

2026 Mar 15;17(1):4002. PMID: 41833955

SU6656 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Mar 15;17(1):4002. [Abstract]

Huh7 cells were pretreated with SU66566 (4 μM) for 30 min before high glucose stimulation (2 h).
Nat Commun

Deficiency of the HGF/Met pathway leads to thyroid dysgenesis by impeding late thyroid expansion. [Abstract]

2024 Apr 11;15(1):3165. PMID: 38605010
Theranostics

HSPA12A unstabilizes CD147 to inhibit lactate export and migration in human renal cell carcinoma. [Abstract]

2020 Jul 9;10(19):8573-8590. PMID: 32754264

SU6656 purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Jul 9;10(19):8573-8590. [Abstract]

Inhibition of FAK phosphorylation by SU6656 (2 µM; 1 h) in Caki-1 cells.

SU6656 purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Jul 9;10(19):8573-8590. [Abstract]

The wound was made in Caki-1 cell layers after knockdown of HSPA12A for 48 h in the presence or absence of SU6656 (2 µM; 1 h), and the migration distance was measured at 24 h after wounding.
Sci Adv

FeaSion decodes the regulatory landscape and functional diversity of RNA polymerase II CTD phosphorylation. [Abstract]

2025 Nov 28;11(48):eadz2345. PMID: 41313762
Cell Rep Med

MFGE8 induces anti-PD-1 therapy resistance by promoting extracellular vesicle sorting of PD-L1. [Abstract]

2025 Jan 16:101922. PMID: 39842432
Cell Chem Biol

The cytotoxic effect of prexasertib is a consequence of dual inhibition on both CHK1 and AMPK. [Abstract]

2026 Jun 18;33(6):767-784.e7. PMID: 42061410
Cell Rep

KIF26B promotes bladder cancer progression via activating Wnt/β-catenin signaling in a TRAF2-dependent pathway. [Abstract]

2025 Apr 19;44(5):115595. PMID: 40253697
J Med Chem

Discovery of Potent Antiallergic Agents Based on an o-Aminopyridinyl Alkynyl Scaffold. [Abstract]

2021 Sep 23;64(18):13588-13603. PMID: 34476950

SU6656 purchased from MedChemExpress. Usage Cited in: J Med Chem. 2021 Sep 23;64(18):13588-13603. [Abstract]

Antidegranulation effects of compound 36 and SU6656 (1.6-100 nM; 1 h).
Cell Biosci

FAK-dependent activation of src family kinase member BLK contributed to endometrial fibrosis via endoplasmic reticulum stress. [Abstract]

2025 Oct 31;15(1):150. PMID: 41174819
J Agric Food Chem

Regulation on Citrate Influx and Metabolism through Inhibiting SLC13A5 and ACLY: A Novel Mechanism Mediating the Therapeutic Effects of Curcumin on NAFLD. [Abstract]

2021 Aug 11;69(31):8714-8725. PMID: 34323067
J Ethnopharmacol

Corydalis saxicola Bunting total alkaloids improve NAFLD by suppressing de novo lipogenesis through the AMPK-SREBP1 axis. [Abstract]

2024 Jan 30;319(Pt 1):117162. PMID: 37690477
Drug Dev Res

Kaempferol suppresses glioma progression and synergistically enhances the antitumor activity of gefitinib by inhibiting the EGFR/SRC/STAT3 signaling pathway. [Abstract]

2023 May;84(3):592-610. PMID: 36852868
Biochim Biophys Acta Mol Basis Dis

Sphingosine kinase 2 cooperating with Fyn promotes kidney fibroblast activation and fibrosis via STAT3 and AKT. [Abstract]

2018 Nov;1864(11):3824-3836. PMID: 30251698

SU6656 purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3824-3836. [Abstract]

NRK-49F cells are pretreated with Fyn inhibitor SU6656 (1 μM) for 30 min, followed by TGF-β1 stimulation for 12 h, and then protein levels of fibronectin and α-SMA are determined by Western blotting.
Biomedicines

SMYD3-CDCP1 Axis Drives EMT and CAF Activation in Colorectal Cancer and Is Targetable for Oxaliplatin Sensitization. [Abstract]

2025 Nov 9;13(11):2737. PMID: 41301830
Fitoterapia

The protective effect and potential mechanism of Zanthoxylum bungeanum Maxim. on atherosclerosis. [Abstract]

2025 Jan 16:106394. PMID: 39826616
Biomed Res Int

Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function. [Abstract]

2022 Oct 25:2022:6006981. PMID: 36330453
MedComm-Oncology

2023 Oct 17.
Connect Tissue Res

Collagens I and V differently regulate the proliferation and adhesion of rat islet INS-1 cells through the integrin β1/E-cadherin/β-catenin pathway. [Abstract]

2020 Dec 1;1-13. PMID: 33957832
J Exp Zool A Ecol Integr Physiol

The Inhibition of PP1γ2 by PKA Through SRC Initiates Sperm Motility in Tree Shrews. [Abstract]

2025 Sep 24. PMID: 40990220
bioRxiv

The nucleus forms a dynamic contact with the plasma membrane to maintain the glandular epithelial architecture. [Abstract]

2025 Jun 15:2025.06.13.659552. PMID: 40661617
bioRxiv

A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug Resistance. [Abstract]

2025 Jun 16:2025.06.11.659120. PMID: 40667115
SSRN

2023 Oct 11.
bioRxiv

2019 May.

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (53.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6922 mL

13.4608 mL

26.9215 mL

5 mM

0.5384 mL

2.6922 mL

5.3843 mL

10 mM

0.2692 mL

1.3461 mL

2.6922 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (5.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Blake RA, et al. SU6656, a selective src family kinase inhibitor, used to probe growth factor signaling. Mol Cell Biol. 2000 Dec;20(23):9018-27. [Content Brief]

[2]. Veracini L, et al. Elevated Src family kinase activity stabilizes E-cadherin-based junctions and collective movement of head and neck squamous cell carcinomas. Oncotarget. 2014 Dec 26. [Content Brief]

[3]. Wu ML, et al. Divergent signaling pathways cooperatively regulate TGFβ induction of cysteine-rich protein 2 in vascular smooth muscle cells. Cell Commun Signal. 2014 Mar 28;12:22. [Content Brief]

[4]. Ondrusová L, et al. Inhibition of mTORC1 by SU6656, the selective Src kinase inhibitor, is not accompanied by activation of Akt/PKB signalling in melanoma cells. Folia Biol (Praha). 2013;59(4):162-7. [Content Brief]

[5]. Kumar A, et al. Pharmacological investigations on possible role of Src kinases in neuroprotective mechanism of ischemic postconditioning in mice. Int J Neurosci. 2014 Oct;124(10):777-86. [Content Brief]

[6]. Liu XF, et al. Antitumor effects of immunotoxins are enhanced by lowering HCK or treatment with SRC kinase inhibitors. Mol Cancer Ther. 2014 Jan;13(1):82-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6922 mL	13.4608 mL	26.9215 mL	67.3038 mL
	5 mM	0.5384 mL	2.6922 mL	5.3843 mL	13.4608 mL
	10 mM	0.2692 mL	1.3461 mL	2.6922 mL	6.7304 mL
	15 mM	0.1795 mL	0.8974 mL	1.7948 mL	4.4869 mL
	20 mM	0.1346 mL	0.6730 mL	1.3461 mL	3.3652 mL
	25 mM	0.1077 mL	0.5384 mL	1.0769 mL	2.6922 mL
	30 mM	0.0897 mL	0.4487 mL	0.8974 mL	2.2435 mL
	40 mM	0.0673 mL	0.3365 mL	0.6730 mL	1.6826 mL
	50 mM	0.0538 mL	0.2692 mL	0.5384 mL	1.3461 mL

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SU6656

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Peptidomimetic Library

Anti-Aging Compound Library

Covalent Screening Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Glutamine Metabolism Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) SU6656

All Src Isoforms

All Akt Isoforms

Biological Activity

Chemical Information

Publications (24)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

SU6656 Related Classifications

HY-B0789 Related Classifications