4'-Methoxychalcone
Based on 1 Customer Validation
4'-Methoxychalcone regulates adipocyte differentiation through PPARγ activation. 4'-Methoxychalcone modulates the expression and secretion of various adipokines in adipose tissue that are involved in insulin sensitivity.
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- Pureté : 99.82%
- CAS No.: 959-23-9
- Formule: C16H14O2
- Masse moléculaire:238.28
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
PPARγ[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | GI50 |
6.8 μM
Compound: 17
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Antiproliferative activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay
Antiproliferative activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay
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[PMID: 34262643] |
| CAL-51 | GI50 |
3.33 μM
Compound: SSE14109
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Antiproliferative activity against human CAL51 cells assessed as growth inhibition after 3 days by SRB assay
Antiproliferative activity against human CAL51 cells assessed as growth inhibition after 3 days by SRB assay
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[PMID: 28743509] |
| DLD-1 | EC50 |
35 μM
Compound: 7
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Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| FHC | CC50 |
62 μM
Compound: 7
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Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HCT-116 | EC50 |
16 μM
Compound: 7
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Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HCT-116 | EC50 |
17 μM
Compound: 7
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Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HCT-116 | GI50 |
3.57 μM
Compound: SSE14109
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Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay
Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay
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[PMID: 28743509] |
| HT-29 | EC50 |
23 μM
Compound: 7
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Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HT-29 | GI50 |
11.7 μM
Compound: 17
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Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay
Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay
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[PMID: 34262643] |
| L929 | CC50 |
50.4 μM
Compound: 13
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Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 31000155] |
| MSTO-211H | GI50 |
11.7 μM
Compound: 17
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Antiproliferative activity against human MSTO-211H cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay
Antiproliferative activity against human MSTO-211H cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay
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[PMID: 34262643] |
In Vitro
4'-Methoxychalcone (5 μM; 8 days) markedly increases the mRNA expression of adipogenic genes during adipocyte differentiation, PPARγ, aP2, FAS, adiponectin and GluT 4[1]. 4'-Methoxychalcone (5 μM; 8 days) reduces the upregulated mRNA expression of IL-6, PAI-1, and MCP-1 by TNF-α in preadipocyte 3T3-L1 cells[1]. 4'-Methoxychalcone (5 μM; 0-8 days) causes an increase in PPARγ expression during differentiation, while C/EBPβ protein expression is relatively unaffected[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Preadipocyte 3T3-L1 cells
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Concentration:5 μM
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Incubation Time:0 day; 2 days; 4 days; 6 days; 8 days
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Result:Increased the mRNA expression of all adipogenic genes, except GluT1 in adipocyte.
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Cell Line:Preadipocyte 3T3-L1 cells
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Concentration:5 μM
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Incubation Time:8 days
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Result:Increased PPARγ expression in adipocyte.
Chemical Information
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CAS No. 959-23-9
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Appearance Solid
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Masse moléculaire 238.28
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Formule C16H14O2
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Color White to light yellow
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SMILES
O=C(C1=CC=C(OC)C=C1)/C=C/C2=CC=CC=C2
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 50 mg/mL (209.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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3T3-L1 preadipocyte-to-adipocyte differentiation
3T3-L1 preadipocytes are induced to differentiate after growth arrest using adipogenic media containing insulin, dexamethasone, and IBMX; differentiation is assessed by lipid-droplet accumulation, triglyceride increase, Oil Red O staining, and adipocyte-marker induction such as PPARγ and C/EBPα.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Large-size fat particle sorting
Large-size fat particle sorting is widely used to isolate cells up to 200 μm in diameter. Single-cell flow sorting will allow greater insight into adipocyte heterogeneity by identifying gene expression, protein composition, and metabolic signatures at the single-cell level.
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Research Protocol for Endocrine Diseases
Endocrine diseases often arise from disrupted hormone production, hormone signaling, or target-tissue responsiveness; for diabetes-focused endocrine disease models, insulin signaling regulates glucose uptake, hepatic glucose output, lipid metabolism, and β-cell compensation. Type 2 diabetes develops through interacting defects in insulin resistance, β-cell dysfunction, adipose inflammation, hepatic glucose overproduction, altered incretin signaling, and ectopic lipid metabolism. A major unresolved question is whether endocrine dysfunction is driven primarily by target-tissue insulin resistance, intrinsic β-cell failure, immune/inflammatory stress, or combined multi-organ failure that differs by disease stage.
Pureté et documentation
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Fiche technique (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1967 mL | 20.9837 mL | 41.9674 mL | 104.9186 mL |
| 5 mM | 0.8393 mL | 4.1967 mL | 8.3935 mL | 20.9837 mL | |
| 10 mM | 0.4197 mL | 2.0984 mL | 4.1967 mL | 10.4919 mL | |
| 15 mM | 0.2798 mL | 1.3989 mL | 2.7978 mL | 6.9946 mL | |
| 20 mM | 0.2098 mL | 1.0492 mL | 2.0984 mL | 5.2459 mL | |
| 25 mM | 0.1679 mL | 0.8393 mL | 1.6787 mL | 4.1967 mL | |
| 30 mM | 0.1399 mL | 0.6995 mL | 1.3989 mL | 3.4973 mL | |
| 40 mM | 0.1049 mL | 0.5246 mL | 1.0492 mL | 2.6230 mL | |
| 50 mM | 0.0839 mL | 0.4197 mL | 0.8393 mL | 2.0984 mL | |
| 60 mM | 0.0699 mL | 0.3497 mL | 0.6995 mL | 1.7486 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5246 mL | 1.3115 mL | |
| 100 mM | 0.0420 mL | 0.2098 mL | 0.4197 mL | 1.0492 mL |
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