Alborixin
Alborixin is a polycyclic polyether ionophore Antibiotic. Alborixin is isolated from cultures of Streptomyces albus. Alborixin induces Autophagy via PTEN-mediated inhibition of the AKT pathway, thereby clearing Amyloid-β. Alborixin exhibits activity against Gram-positive bacteria and fungi. Alborixin can be used in the research of Alzheimer's disease.
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- CAS No.: 57760-36-8
- Formule: C48H84O14
- Masse moléculaire:885.17
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Activité biologique
Alborixin (30-125 nM; 3-24 h) induces autophagy in mouse microglial N9 cells, with the maximal effect observed after treatment with 125 nM for 24 h; its mechanism of action involves upregulating LC3B-II levels, downregulating SQSTM1 levels, promoting GFP-LC3 puncta formation and increasing the number of autophagosomes[1].
Alborixin (125 nM) enhances soluble and fibrillar Aβ-induced autophagy in mouse microglial N9 cells, as evidenced by increased LC3B-II levels and decreased SQSTM1 levels[1].
Alborixin (125-250 nM; 12-24 h) eliminates soluble Aβ and fibrillary Aβ in mouse microglial N9 cells and human microglial HMC3 cells, and clears soluble Aβ in primary mouse neurons at 250 nM for 24 h. Its mechanism of action relies on the PTEN-AKT-dependent autophagy pathway, which requires functional ATG5 and BECN1[1].
Alborixin exhibits activity against Gram-positive bacteria and fungi[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:mouse microglial N9 cells
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Concentration:30 nM; 125 nM
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Incubation Time:12 h; 3 h, 6 h, 12 h, 24 h
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Result:Induced autophagy in a concentration-dependent manner, with increased LC3B-II levels and decreased SQSTM1 levels detectable at 30 nM for 12 h.
Increased LC3B-II:ACTB ratio in a time-dependent manner at 125 nM for 3 h, 6 h, 12 h, 24 h, while decreasing SQSTM1:ACTB ratio.
Confirmed autophagy flux via further increases in LC3B-II levels when combined with bafilomycin A1.
Increased GFP-LC3 puncta per cell.
Increased average autophagosomes per cell.
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Cell Line:mouse microglial N9 cells
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Concentration:125 nM
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Incubation Time:3 h, 6 h, 9 h, 12 h, 24 h
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Result:Upregulated autophagy-related proteins BECN1, ATG7, ATG5, and ATG12 in a time-dependent manner, with peak expression at 24 h.
Increased PTEN:ACTB ratio in a time-dependent manner.
Reduced the ratios of p-AKT (S473):AKT, p-AKT (T308):AKT, p-MTOR (S2448):MTOR, and RPTOR:ACTB over 24 h.
Chemical Information
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CAS No. 57760-36-8
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Masse moléculaire 885.17
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Formule C48H84O14
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SMILES
O[C@@]1([C@]2(O[C@](CC2)([H])[C@H]([C@@]3(O[C@@H]([C@H](C[C@H]3C)C)C[C@]4(O[C@@](CC[C@@H]4C)([H])[C@@H](C)[C@H](O)C[C@@H]5O[C@]([C@H](C[C@H]5C)C)([H])[C@@H](C)C(O)=O)O)O)O)C)O[C@@](C[C@H]1C)([C@]6([H])O[C@H]([C@](O)(CC6)C)CC)C
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Structure Classification
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Initial Source
streptomyces scabrisporus
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Wani A, et al. Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway. Autophagy. 2019;15(10):1810-1828. [Content Brief]
[2]. Gachon P, et al. Alborixin, a new antibiotic ionophore: isolation, structure, physical and chemical properties. J Antibiot (Tokyo). 1976;29(6):603-610. [Content Brief]
Calculators
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